Microfluidic cold-finger device for the investigation of antifreeze proteins

Haleva, Lotem; Celik, Yeliz; Bar-Dolev, Maya; Kaner, Avigail; Pertaya, Natalya; Davies, Peter L.; Braslavsky, Ido

doi:10.1016/j.cryobiol.2016.09.160

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We named this apparatus the microfluidic cold finger (MCF) device. A detailed description of the system is given elsewhere [29]. The control over the temperature in the MCF is both from the stage below the microfluidic chip and from the centre of a chamber located in the middle of the microfluidic channel.…”

Section: Adhesion To Ice In Microfluidic Systemsmentioning

confidence: 99%

Putting life on ice: bacteria that bind to frozen water

Dolev

Bernheim

Guo

et al. 2016

J. R. Soc. Interface.

View full text Add to dashboard Cite

Ice-binding proteins (IBPs) are typically small, soluble proteins produced by cold-adapted organisms to help them avoid ice damage by either resisting or tolerating freezing. By contrast, the IBP of the Antarctic bacterium Marinomonas primoryensis is an extremely long, 1.5 MDa protein consisting of five different regions. The fourth region, a 34 kDa domain, is the only part that confers ice binding. Bioinformatic studies suggest that this IBP serves as an adhesin that attaches the bacteria to ice to keep it near the top of the water column, where oxygen and nutrients are available. Using temperature-controlled cells and a microfluidic apparatus, we show that M. primoryensis adheres to ice and is only released when melting occurs. Binding is dependent on the mobility of the bacterium and the functionality of the IBP domain. A polyclonal antibody raised against the IBP region blocks bacterial ice adhesion. This concept may be the basis for blocking biofilm formation in other bacteria, including pathogens. Currently, this IBP is the only known example of an adhesin that has evolved to bind ice.

show abstract

Section: Adhesion To Ice In Microfluidic Systemsmentioning

confidence: 99%