Microfluidic colorimetric biosensor for rapid and sensitive detection of Shewanella oneidensis MR‑1

Jiang, Mingxia; Lin, Yuyin; Xu, Liyuan; Chen, Zhihang; Wu, Junxian; Yuan, Yong; Zhou, Lihua

doi:10.1016/j.snb.2024.136872

Sensors and Actuators B: Chemical

2025

DOI: 10.1016/j.snb.2024.136872

|View full text |Cite

Microfluidic colorimetric biosensor for rapid and sensitive detection of Shewanella oneidensis MR‑1

Mingxia Jiang,

Yuyin Lin,

Liyuan Xu

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

A Microfluidic Biosensor for Quantitative Detection of Salmonella in Traditional Chinese Medicine

Wu,

Liu,

et al. 2024

Biosensors

View full text Add to dashboard Cite

Microbial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and sensitive detection of Salmonella in traditional Chinese medicine on a microfluidic chip. Immune gold@platinum nanocatalysts (Au@PtNCs) were utilized for specific bacterial labeling, while magnetic nano-beads (MNBs) with a novel high-gradient magnetic field were employed for the specific capture of bacteria. The immune MNBs, immune Au@PtNCs, and bacterial samples were introduced into a novel passive microfluidic micromixer for full mixing, resulting in the formation of a double-antibody sandwich structure due to antigen–antibody immune reactions. Subsequently, the mixture flowed into the reaction cell, where the MNBs-Salmonella-Au@PtNCs complex was captured by the magnetic field. After washing, hydrogen peroxide-tetramethylbenzidine substrate (H2O2-TMB) was added, reacting with the Au@PtNCs peroxidase to produce a blue reaction product. This entire process was automated using a portable device, and Salmonella concentration was analyzed via a phone application. This simple biosensor has good specificity with a detection range of 9 × 101–9 × 105 CFU/mL and can detect Salmonella concentrations as low as 90 CFU/mL within 74 min. The average recoveries of the spiked samples ranged from 76.8% to 109.5%

show abstract

A Microfluidic Biosensor for Quantitative Detection of Salmonella in Traditional Chinese Medicine

Wu,

Liu,

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Microfluidic colorimetric biosensor for rapid and sensitive detection of Shewanella oneidensis MR‑1

Cited by 1 publication

References 37 publications

A Microfluidic Biosensor for Quantitative Detection of Salmonella in Traditional Chinese Medicine

A Microfluidic Biosensor for Quantitative Detection of Salmonella in Traditional Chinese Medicine

Contact Info

Product

Resources

About