Microfluidic device for sheathless particle focusing and separation using a viscoelastic fluid

Nam, Jeonghun; Namgung, Bumseok; Lim, Chwee Teck; Bae, Jung-Eun; Leo, Hwa Liang; Cho, Kwang Soo; Kim, Sangho

doi:10.1016/j.chroma.2015.06.029

Cited by 67 publications

(66 citation statements)

References 45 publications

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“…A similar idea was later demonstrated by Yuan et al [125] to extract plasma from diluted blood samples in PEO solutions via the 3D elasto-inertial focusing of blood cells in a straight rectangular microchannel with an array of triangular side-wells along one sidewall. In another study Nam et al [126] proposed a two-stage microfluidic device for sheath-free particle separation in a viscoelastic fluid flow (Fig. 10b).…”

Section: Sheath-free Separation By Sizementioning

confidence: 99%

“…Particles are elasto-inertially focused to the centerline of a circular microchannel at the 1st stage and subsequently deflected by the elastic lift to size-dependent flow paths in two symmetrically arranged rectangular microchannels at the 2nd stage. The device was first tested with the separation of 5 mm and 10 mm diameter PS particles in a highly elastic 8% PVP solution at very low flow rates with Re < 0:01 [126]. It was later improved for the separation of tumor cells from leukocytes in a weakly elastic 0.1% HA solution at a throughput of 4 Â 10 5 cells/min (200 ml/min) [127].…”

Section: Sheath-free Separation By Sizementioning

confidence: 99%

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Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

247

192

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a b s t r a c tMicrofluidic devices have been widely used since 1990s for diverse manipulations of particles (a general term of beads, cells, vesicles, drops, etc.) in a variety of applications. Compared to the active manipulation via an externally imposed force field, the passive manipulation of particles exploits the flow-induced intrinsic lift and/or drag to control particle motion with several advantages. Along this direction, inertial microfluidics has received tremendous interest in the past decade due to its capability to handle a large volume of samples at a high throughput. This inertial lift-based approach in Newtonian fluids, however, becomes ineffective and even fails for small particles and/or at low flow rates. Recent studies have demonstrated the potential of elastic lift in non-Newtonian fluids for manipulating particles with a much smaller size and over a much wider range of flow rates. The aim of this article is to provide an overview of the various passive manipulations, including focusing, separation, washing and stretching, of particles that have thus far been demonstrated in non-Newtonian microfluidics.

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Section: Sheath-free Separation By Sizementioning

confidence: 99%

Section: Sheath-free Separation By Sizementioning

confidence: 99%

Particle manipulations in non-Newtonian microfluidics: A review

Liu

et al. 2017

Journal of Colloid and Interface Science

247

192

View full text Add to dashboard Cite

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“…However, sheath ow is not benecial for channel parallelization design and high-throughput processing. Instead of using sheath ow, Nam et al 36,37 used specic channel geometry to align particles before separation. He used a circular channel followed by a symmetric bifurcation channel and a sudden expansion region to realize the initialization of the particle position and continuous particle separation in elasticity dominant polyvinyl pyrrolidone (PVP) uid.…”

Section: 18mentioning

confidence: 99%

Sheathless Dean-flow-coupled elasto-inertial particle focusing and separation in viscoelastic fluid

et al. 2017

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In this paper, a novel microfluidic device for sheathless particle focusing and separation in viscoelastic fluid is proposed. The device consists of two stages: a straight channel section with asymmetrical expansioncontraction cavity arrays (ECCA section) for sheathless Dean-flow-coupled elasto-inertial particle focusing (1st stage), and a straight channel section for viscoelastic particle separation (2nd stage). In stage 1, particles with diameters of 5 mm and 13 mm were both focused at the opposite sides of the cavities. Then, the particles were subsequently separated at the 2nd stage based on the differential focusing dependency on size. The effects of flow rates and channel length on particle separation were investigated. Particle separation in both viscoelastic fluid and Newtonian fluid was also compared to elucidate the differences. In addition, particle separation in the straight channel and integrated ECCA straight channel was also studied. The proposed device was used to separate human Jurkat cells (an immortalized T cell line) and yeast cells. Experimental results show that this technique offers an efficient, continuous, and sheathless particle separation in viscoelastic fluid.

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“…Focusing randomly distributed particles into one or several positions can be employed to enrich 1 or filtrate 2 bioparticles. Separation of target bioparticles from a massive background of bioparticles according to the unique biophysical properties is a routine process in medical laboratories for down-stream biochemical analysis, disease diagnosis and therapeutics 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the elastic property of non-Newtonian fluid has been extensively studied to manipulate micro-particles in microfluidics 27,28 . The proposed viscoelastic mediums for particle viscoelastic focusing include solutions of poly(ethylene oxide) (PEO) [29][30][31][32] , polyvinylpyrrolidone (PVP) 3,33 , DNA 34 , polyacrylamide (PAM) 35 and hyaluronic acid (HA) 36 . The elastic force induced by the normal stress difference in these viscoelastic fluids will focus particles into the zero shear rate regions (channel centerline or corners in a square channel) 34,37 .…”

Section: Introductionmentioning

confidence: 99%

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

et al. 2016

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Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes the particle sample. In this work, we propose and develop a novel viscoelastic ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of nonmagnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices. Disciplines Engineering | Science and Technology Studies Abstract:Separation of microparticles has found broad applications in biomedicine, industry and clinical diagnosis. In a conventional aqueous ferrofluid, separation of microparticles usually employs a sheath flow or two offset magnets to confine particle streams for downstream particle sorting. This complicates the fluid control, device fabrication, and dilutes particle sample. In this work, we propose and develop a novel viscoelastic-based ferrofluid by replacing the Newtonian base medium of the conventional ferrofluid with non-Newtonian poly(ethylene oxide) (PEO) aqueous solution. The properties of both viscoelastic 3D focusing and negative magnetophoresis of the viscoelastic-based ferrofluid were verified and investigated. By employing the both properties in a serial manner, continuous and sheathless separation of non-magnetic particles based on particle size has been demonstrated. This novel viscoelastic ferrofluid is expected to bring more flexibility and versatility to the design and functionality in microfluidic devices.

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Microfluidic device for sheathless particle focusing and separation using a viscoelastic fluid

Cited by 67 publications

References 45 publications

Particle manipulations in non-Newtonian microfluidics: A review

Particle manipulations in non-Newtonian microfluidics: A review

Sheathless Dean-flow-coupled elasto-inertial particle focusing and separation in viscoelastic fluid

A novel viscoelastic-based ferrofluid for continuous sheathless microfluidic separation of nonmagnetic microparticles

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