Microfluidic devices for cellomics: a review

Andersson, Helene; Berg, Albert van den

doi:10.1016/s0925-4005(03)00266-1

Cited by 604 publications

(404 citation statements)

References 92 publications

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“…8,9 Currently available approaches to on-the-chip sample preparation are based on filtration4 , 8 ,33 or dielectrophoresis (DEP) 34 or require preliminary cell modification steps such as fluorescent labeling35 , 36 or tagging with magnetic beads.37 ,38 The current state of the art of these and other sample preparation methods is the focus of recent reviews. 3,6,7,10 The new leukocyte enrichment method described here has many important advantages over these technologies. It is simple, has no active (moving or otherwise) elements, requires only a small hydrostatic pressure gradient to function, and can be easily manufactured using conventional microfabrication.…”

Section: Resultsmentioning

confidence: 99%

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Biomimetic Autoseparation of Leukocytes from Whole Blood in a Microfluidic Device

et al. 2004

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Leukocytes comprise less than 1% of all blood cells. Enrichment of their number, starting from a sample of whole blood, is the required first step of many clinical and basic research assays. We created a microfluidic device that takes advantage of the intrinsic features of blood flow in the microcirculation, such as plasma skimming and leukocyte margination, to separate leukocytes directly from whole blood. It consists of a simple network of rectangular microchannels designed to enhance lateral migration of leukocytes and their subsequent extraction from the erythrocytedepleted region near the sidewalls. A single pass through the device produces a 34-fold enrichment of the leukocyte-to-erythrocyte ratio. It operates on microliter samples of whole blood, provides positive, continuous flow selection of leukocytes, and requires neither preliminary labeling of cells nor input of energy (except for a small pressure gradient to support the flow of blood). This effortless, efficient, and inexpensive technology can be used as a lab-on-a-chip component for initial whole blood sample preparation. Its integration into microanalytical devices that require leukocyte enrichment will enable accelerated transition of these devices into the field for point-of-care clinical testing.The rapidly expanding field of lab-on-a-chip microanalytical devices promises inexpensive, portable, miniaturized tools that could potentially revolutionize clinical and basic research analyses by dramatically reducing sample size and handling.1 -3 An important application of this technology is the analysis of leukocytes (white blood cells) or their contents, for which these cells must first be isolated from the whole blood sample. [4][5][6][7] Blood is a 45% suspension of cells (erythrocytes, platelets, leukocytes) in plasma. Erythrocytes (red blood cells) constitute the vast majority of all blood cells and are normally ∼1000 times more abundant than leukocytes. Traditionally, several milliliters of whole blood are drawn and then centrifuged in order to separate blood cells of different density. Plasma, platelets, and white and red cells can be separated by this procedure, but it is a labor-, energy-, and time-intensive process that relies on special equipment and requires trained personnel. 8, 9 Many current microanalytical devices have no integrated sample

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Section: Resultsmentioning

confidence: 99%

“…[4][5][6][7] Blood is a 45% suspension of cells (erythrocytes, platelets, leukocytes) in plasma. Erythrocytes (red blood cells) constitute the vast majority of all blood cells and are normally ∼1000 times more abundant than leukocytes.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Autoseparation of Leukocytes from Whole Blood in a Microfluidic Device

et al. 2004

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“…In particular, they provide very tightly controlled and tailored environments for the culture of cells [1,2], tissues [3], and small organisms [4]. In principle, NMR spectroscopy would complement such systems very well, enabling non-destructive and non-invasive observation of metabolic processes over a period of time.…”

Section: Introductionmentioning

confidence: 99%

An optimised detector for in-situ high-resolution NMR in microfluidic devices

Finch

Yılmaz

Utz

2016

Journal of Magnetic Resonance

109

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Integration of high-resolution nuclear magnetic resonance (NMR) spectroscopy with microfluidic lab-on-a-chip devices is challenging due to limited sensitivity and line broadening caused by magnetic susceptibility inhomogeneities. We present a novel double-stripline NMR probe head that accommodates planar microfluidic devices, and obtains the NMR spectrum from a rectangular sample chamber on the chip with a volume of 2 l. Finite element analysis is used to jointly optimise the detector and sample volume geometry for sensitivity and RF homogeneity. A prototype of the optimised design has been built, and its properties have been characterised experimentally. The performance in terms of sensitivity and RF homogeneity closely agrees with the numerical predictions. The system reaches a mass limit of detection of 1.57 nMol p s, comparing very favourably with other micro-NMR systems. The spectral resolution of this chipGprobe system is better than 1.75 Hz at a magnetic field of 7 T, with excellent line shape.

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“…1 These microdevices are advantageous in that they use very small volumes of reagents and can be potentially scaled up for high-throughput analysis. Although great progress has been made in the fabrication of cell-based biosensors and high-throughput screening approaches, more research in interfacing cells with such microdevices is of benefit.…”

Section: Introductionmentioning

confidence: 99%

Molded polyethylene glycol microstructures for capturing cells within microfluidic channels

et al. 2004

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The ability to control the deposition and location of adherent and non-adherent cells within microfluidic devices is beneficial for the development of micro-scale bioanalytical tools and high-throughput screening systems. Here, we introduce a simple technique to fabricate poly(ethylene glycol) (PEG) microstructures within microfluidic channels that can be used to dock cells within pre-defined locations. Microstructures of various shapes were used to capture and shear-protect cells despite medium flow in the channel. Using this approach, PEG microwells were fabricated either with exposed or non-exposed substrates. Proteins and cells adhered within microwells with exposed substrates, while non-exposed substrates prevented protein and cell adhesion (although the cells were captured inside the features). Furthermore, immobilized cells remained viable and were stained for cell surface receptors by sequential flow of antibodies and secondary fluorescent probes. With its unique strengths in utility and control, this approach is potentially beneficial for the development of cell-based analytical devices and microreactors that enable the capture and real-time analysis of cells within microchannels, irrespective of cell anchorage properties.

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Microfluidic devices for cellomics: a review

Cited by 604 publications

References 92 publications

Biomimetic Autoseparation of Leukocytes from Whole Blood in a Microfluidic Device

Biomimetic Autoseparation of Leukocytes from Whole Blood in a Microfluidic Device

An optimised detector for in-situ high-resolution NMR in microfluidic devices

Molded polyethylene glycol microstructures for capturing cells within microfluidic channels

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