Microfluidic devices for continuous production of pDNA/cationic liposome complexes for gene delivery and vaccine therapy

Balbino, Tiago Albertini; Azzoni, Adriano Rodrigues; Torre, Lucimara Gaziola de la

doi:10.1016/j.colsurfb.2013.04.003

Cited by 64 publications

(48 citation statements)

References 53 publications

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“…Nevertheless, applied flow rate ratios between 10 to 60 will lead to a dilution of the final liposome formulation and might necessitate a concentration step post formation. Flow rates reported are as low as 200 mm/sec [116,117].…”

Section: Manufacture Of Liposomal Adjuvants Using Microfluidicsmentioning

confidence: 89%

Designing liposomal adjuvants for the next generation of vaccines

Perrie

Crofts

Devitt

et al. 2016

Advanced Drug Delivery Reviews

114

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Liposomes not only offer the ability to enhance drug delivery, but can effectively act as vaccine delivery systems and adjuvants. Their flexibility in size, charge, bilayer rigidity and composition allow for targeted antigen delivery via a range of administration routes. In the development of liposomal adjuvants, the type of immune response promoted has been linked to their physico-chemical characteristics, with the size and charge of the liposomal particles impacting on liposome biodistribution, exposure in the lymph nodes and recruitment of the innate immune system. The addition of immunostimulatory agents can further potentiate their immunogenic properties. Here, we outline the attributes that should be considered in the design and manufacture of liposomal adjuvants for the delivery of sub-unit and nucleic acid based vaccines.

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Section: Manufacture Of Liposomal Adjuvants Using Microfluidicsmentioning

confidence: 89%

Designing liposomal adjuvants for the next generation of vaccines

Perrie

Crofts

Devitt

et al. 2016

Advanced Drug Delivery Reviews

114

View full text Add to dashboard Cite

show abstract

“…Adjusting W f or R provides a direct means for determining the mixing time and therefore tuning particle size. Up to now, a great number of self‐assemblies with well‐controlled size and narrow polydispersity have been synthesized in similar 2D HFF devices: polyplexes, liposomes, polymeric micelles, and polymer/lipid hybrid nanoparticles (NPs) . Generally, these findings show that increasing the R value increases the mixing time and results an increase in particle size and distribution.…”

Section: Microfluidics For Fabrication Of Sddss With Well‐controlled mentioning

confidence: 99%

Microfluidics for Cancer Nanomedicine: From Fabrication to Evaluation

Zhang

Zhu

Shen

2018

Small

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Self-assembled drug delivery systems (sDDSs), made from nanocarriers and drugs, are one of the major types of nanomedicines, many of which are in clinical use, under preclinical investigation, or in clinical trials. One of the hurdles of this type of nanomedicine in real applications is the inherent complexity of their fabrication processes, which generally lack precise control over the sDDS structures and the batch-to-batch reproducibility. Furthermore, the classic 2D in vitro cell model, monolayer cell culture, has been used to evaluate sDDSs. However, 2D cell culture cannot adequately replicate in vivo tissue-level structures and their highly complex dynamic 3D environments, nor can it simulate their functions. Thus, evaluations using 2D cell culture often cannot correctly correlate with sDDS behaviors and effects in humans. Microfluidic technology offers novel solutions to overcome these problems and facilitates studying the structure-performance relationships for sDDS developments. In this Review, recent advances in microfluidics for 1) fabrication of sDDSs with well-defined physicochemical properties, such as size, shape, rigidity, and drug-loading efficiency, and 2) fabrication of 3D-cell cultures as "tissue/organ-on-a-chip" platforms for evaluations of sDDS biological performance are in focus.

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“…The polydimethylsiloxane 98 (PDMS)/glass microfluidic devices were then produced following the procedure 99 described in (Balbino et al, 2013b), which is based on the conventional UV 100 photolithographic and soft-lithography methods. The cross-section of the microchannels 101 was rectangular in all cases, with a depth of 50 µm.…”

Section: Production Of the Microfluidic Mixing Devices (Micromixers) 96mentioning

confidence: 99%