Microfluidic Diffusion-Based Separation and Detection

Weigl, Bernhard H.; Yager, Paul

doi:10.1126/science.283.5400.346

Cited by 503 publications

(362 citation statements)

References 5 publications

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“…Micro-mixers are also used as a tool for dispersing immiscible liquids and forming micro-droplets [25], or as a separator for particles based on their different diffusion coefficients [26]. Micro-mixers are classified into two types, active and passive mixers.…”

Section: Micro-mixers Evaluationmentioning

confidence: 99%

Fast Fabrication Process of Microfluidic Devices Based on Cyclic Olefin Copolymer

Azouz

Murphy

Karazi

et al. 2014

Materials and Manufacturing Processes

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A new low-cost process for fast fabrication of multilayer microfluidic devices using cyclic olefin copolymer film materials is presented. This novel process consists of the fabrication of microfluidic features by xurography, followed by multilayer lamination via cyclohexane vapor exposure. Exposure time to this solvent and compression time were optimized for bond tensile strength. A three-layer microfluidic chip capable of withstanding back pressures up to 23 MPa was fabricated in less than an hour. The suitability of this fast prototyping method for fabrication of functional UV-transparent microfluidic devices was demonstrated by development and testing of a microfluidic mixer and preparation of a polymer monolithic column within the microfluidic channel.

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Section: Micro-mixers Evaluationmentioning

confidence: 99%

Fast Fabrication Process of Microfluidic Devices Based on Cyclic Olefin Copolymer

Azouz

Murphy

Karazi

et al. 2014

Materials and Manufacturing Processes

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“…These relatively sharp fluid -fluid interfaces in a steady flow system allow magnetic beads to be transferred between different proximal chemical solutions without switching flows. The use of parallel flows in microreactors is now finding increasing acceptance for a wider range of applications (Weigl and Yager, 1999).…”

Section: Selection Transfer Modulementioning

confidence: 99%

Optically programming DNA computing in microflow reactors

McCaskill¹

2001

Biosystems

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The programmability and the integration of biochemical processing protocols are addressed for DNA computing using photochemical and microsystem techniques. A magnetically switchable selective transfer module (STM) is presented which implements the basic sequence-specific DNA filtering operation under constant flow. Secondly, a single steady flow system of STMs is presented which solves an arbitrary instance of the maximal clique problem of given maximum size N. Values of N up to about 100 should be achievable with current lithographic techniques. The specific problem is encoded in an initial labeling pattern of each module with one of 2N DNA oligonucleotides, identical for all instances of maximal clique. Thirdly, a method for optically programming the DNA labeling process via photochemical lithography is proposed, allowing different problem instances to be specified. No hydrodynamic switching of flows is required during operation -the STMs are synchronously clocked by an external magnet. An experimental implementation of this architecture is under construction and will be reported elsewhere.

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“…Eqn (1) Eqn (2) where c Ab is the antibody concentration in the bulk of the microchannel, c s is the surface concentration of bound antibody, θ is the surface concentration of antibody binding sites, θ 0 is the initial surface concentration of antibody binding sites, and k ads and k des are the adsorption and desorption kinetic parameters.…”

Section: Introductionmentioning

confidence: 99%

Concentration Gradient Immunoassay. 2. Computational Modeling for Analysis and Optimization

et al. 2007

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A novel microfluidic surface-based competition immunoassay, termed the concentration gradient immunoassay (CGIA, described in detail in a companion paper 1 ) uses surface plasmon resonance (SPR) imaging to rapidly measure the concentration of small molecules. To conduct this assay, antibody and analyte are introduced into the two inlets of a T-sensor. 2, 3 Several millimeters downstream, antibody molecules with open binding sites can bind to a surface functionalized with immobilized antigen. This space-and time-dependent binding can be sensitively observed using SPR imaging. In this paper, we describe a complex three-dimensional finite element model developed to better understand the dynamic processes occurring with this assay. The model shows strong qualitative agreement with experimental results for small molecule detection. The model confirms the experimental finding that the position within the microchannel at which the antibody binds to the immobilized analyte may be used to quantify the concentration of analyte. In addition, the model was used to explore the sensitivity of assay performance to parameters such as antibody and analyte concentrations, thereby giving insight into ways to optimize analysis speed and accuracy. Given the experimental verification of the computational results, this model serves as an efficient method to explore the influence of the flow rate, microchannel dimensions, and antibody concentration on the sensitivity of the assay.

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Microfluidic Diffusion-Based Separation and Detection

Cited by 503 publications

References 5 publications

Fast Fabrication Process of Microfluidic Devices Based on Cyclic Olefin Copolymer

Fast Fabrication Process of Microfluidic Devices Based on Cyclic Olefin Copolymer

Optically programming DNA computing in microflow reactors

Concentration Gradient Immunoassay. 2. Computational Modeling for Analysis and Optimization

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