Microfluidic fluorescence immunosensor using ZnONFs for invasive aspergillosis determination

Piguillem, Sofía V.; Regiart, Matías; Bertotti, Mauro; Raba, Julio; Messina, Germán A.; Fernández‐Baldo, Martín A.

doi:10.1016/j.microc.2020.105371

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…Besides, nanomaterials-based miniaturized biosensors possessed outstanding advantages in portable monitoring and high-throughput analytical manner [62][63][64]. Therefore, Piguillem et al prepared the ZnO nanoflowers (ZnONFs), conjugated the nanomaterials to microfluidic channel, and employed the nanocomplex for antibody modification [61]. Correspondingly, a novel immunosensor was established for fluorescent detection of GM via the generation of fluorescent substance resorufin (Figure 5C).…”

Section: Galactomannan-related Biomarkermentioning

confidence: 99%

Nanomaterials-Based Biosensors against Aspergillus and Aspergillosis: Control and Diagnostic Perspectives

Guo,

Zhang,

Wang

et al. 2023

Infectious Diseases

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Aspergillosis is the name given to the spectrum of diseases caused by the genus Aspergillus. Research on aspergillosis has shown a progressive expansion over the past decades, largely due to the rise in the number of immunocompromised individuals who are at risk for the infection. Nanotechnology provides innovative tools in the medicine, diagnosis, and treatment. The unique properties of nanomaterials like small size in the nanoscale have attracted researchers to explore their potential, especially in medical diagnostics. Aptamers, considered as chemical antibody, are short, single-stranded oligonucleotide molecules with high affinity and specificity to interact with target molecules even superior to antibody. Accordingly, development of nanomaterials-based biosensors technology such as immunosensors and aptasensors against Aspergillus and Aspergillosis is of great significance and urgency. In this book chapter, we comprehensively introduce and analyze the recent progress of nanomaterials-based biosensors against Aspergillus and Aspergillosis. In addition, we reveal the challenges and provide our opinion in future opportunities for such sensing platform development. Ultimately, conclusion and future prospects are highlighted and summarized.

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Section: Galactomannan-related Biomarkermentioning

confidence: 99%

Nanomaterials-Based Biosensors against Aspergillus and Aspergillosis: Control and Diagnostic Perspectives

Guo,

Zhang,

Wang

et al. 2023

Infectious Diseases

View full text Add to dashboard Cite

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“…To improve the sensitivity of fluorescent microfluidic immunoassay, integration of nanostructures with microchannels is a promising approach. At present, ZnO has been broadly used in fluorescence immunoassays because of its fluorescence amplification ability towards biomolecular (Hu et al, 2015;Liu et al, 2016;Piguillem et al, 2020). Guo et al grew ZnO nanowires in microfluidic channels for enhanced fluorescent detection of cancer biomarkers.…”

Section: Fluorescent Microfluidic Immunoassaymentioning

confidence: 99%

Recent progress of microfluidic chips in immunoassay

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Microfluidic chip technology is a technology platform that integrates basic operation units such as processing, separation, reaction and detection into microchannel chip to realize low consumption, fast and efficient analysis of samples. It has the characteristics of small volume need of samples and reagents, fast analysis, low cost, automation, portability, high throughout, and good compatibility with other techniques. In this review, the concept, preparation materials and fabrication technology of microfluidic chip are described. The applications of microfluidic chip in immunoassay, including fluorescent, chemiluminescent, surface-enhanced Raman spectroscopy (SERS), and electrochemical immunoassay are reviewed. Look into the future, the development of microfluidic chips lies in point-of-care testing and high throughput equipment, and there are still some challenges in the design and the integration of microfluidic chips, as well as the analysis of actual sample by microfluidic chips.

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“…Optical immunosensors are based on the generation of an optical signal (color [38][39][40] or fluorescence [41][42][43]) or upon changes in the local optical properties (absorption or emission of light, refractive index, etc.) after the Ab/Ag complex formation.…”

Section: From Biosensors To Immunosensors: Definitions and Type Of Tr...mentioning

confidence: 99%

Addressing the Theoretical and Experimental Aspects of Low-Dimensional-Materials-Based FET Immunosensors: A Review

et al. 2021

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Electrochemical immunosensors (EI) have been widely investigated in the last several years. Among them, immunosensors based on low-dimensional materials (LDM) stand out, as they could provide a substantial gain in fabricating point-of-care devices, paving the way for fast, precise, and sensitive diagnosis of numerous severe illnesses. The high surface area available in LDMs makes it possible to immobilize a high density of bioreceptors, improving the sensitivity in biorecognition events between antibodies and antigens. If on the one hand, many works present promising results in using LDMs as a sensing material in EIs, on the other hand, very few of them discuss the fundamental interactions involved at the interfaces. Understanding the fundamental Chemistry and Physics of the interactions between the surface of LDMs and the bioreceptors, and how the operating conditions and biorecognition events affect those interactions, is vital when proposing new devices. Here, we present a review of recent works on EIs, focusing on devices that use LDMs (1D and 2D) as the sensing substrate. To do so, we highlight both experimental and theoretical aspects, bringing to light the fundamental aspects of the main interactions occurring at the interfaces and the operating mechanisms in which the detections are based.

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Microfluidic fluorescence immunosensor using ZnONFs for invasive aspergillosis determination

Cited by 7 publications

References 41 publications

Nanomaterials-Based Biosensors against Aspergillus and Aspergillosis: Control and Diagnostic Perspectives

Nanomaterials-Based Biosensors against Aspergillus and Aspergillosis: Control and Diagnostic Perspectives

Recent progress of microfluidic chips in immunoassay

Addressing the Theoretical and Experimental Aspects of Low-Dimensional-Materials-Based FET Immunosensors: A Review

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