Microfluidic Impedance Flow Cytometry Enabling  High-Throughput Single-Cell Electrical  Property Characterization

Chen, Jian; Chen, Xue; Zhao, Yang; Chen, Deyong; Wu, Min‐Hsien; Wang, Junbo

doi:10.3390/ijms16059804

Cited by 135 publications

(109 citation statements)

References 129 publications

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“…Furthermore, this tool was used for quantifying cellular electrical properties since the deformed cells effectively seal constriction channel walls, and block electric lines, enabling single-cell electrical property characterization [53][54][55][56]. Meanwhile, the constriction channel design was also used as a tool to study chemical synthesis of red blood cells [57] or deliver vector-free gene vectors [42,58].…”

Section: Discussionmentioning

confidence: 99%

Constriction Channel Based Single-Cell Mechanical Property Characterization

et al. 2015

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This mini-review presents recent progresses in the development of microfluidic constriction channels enabling high-throughput mechanical property characterization of single cells. We first summarized the applications of the constriction channel design in quantifying mechanical properties of various types of cells including red blood cells, white blood cells, and tumor cells. Then we highlighted the efforts in modeling the cellular entry process into the constriction channel, enabling the translation of raw mechanical data (e.g., cellular entry time into the constriction channel) into intrinsic cellular mechanical properties such as cortical tension or Young's modulus. In the end, current limitations and future research opportunities of the microfluidic constriction channels were discussed.

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Section: Discussionmentioning

confidence: 99%

Constriction Channel Based Single-Cell Mechanical Property Characterization

et al. 2015

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“…Microfluidic Impedance Cytometry (MIC) is a widely used labelfree technique for high throughput single-cell electrical analysis and discrimination [2][3][4] . The impedance of single particles is measured by applying an AC voltage to two pairs of electrodes and measuring the differential current as a cell transits through the system.…”

Section: Introductionmentioning

confidence: 99%

High accuracy particle analysis using sheathless microfluidic impedance cytometry

et al. 2016

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This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device using two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 µm particles are collected and compared with simulations. The method gives excellent Coefficient of Variation in (electrical) radius of particles of 1% for a sheath less configuration.

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“…(1) Cell Encapsulation and Differentiation: Heterogeneous cells are isolated, encapsulated inside droplets, and differentiated according to their identity (type); e.g., their shape, size, cell-cycle stage, or lineage [3]. (2) Droplet Indexing (Barcoding): Each droplet is manipulated through a sequence of biochemical procedures such as cell lysis and mRNA analysis.…”

Section: Introductionmentioning

confidence: 99%

CoSyn: Efficient single-cell analysis using a hybrid microfluidic platform

Ibrahim

Chakrabarty

Schlichtmann

2017

Design, Automation &Amp; Test in Europe Conference &Amp; Exhibition (DATE), 2017

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Abstract-Single-cell genomics is used to advance our understanding of diseases such as cancer. Microfluidic solutions have recently been developed to classify cell types or perform singlecell biochemical analysis on pre-isolated types of cells. However, new techniques are needed to efficiently classify cells and conduct biochemical experiments on multiple cell types concurrently. System integration and design automation are major challenges in this context. To overcome these challenges, we present a hybrid microfluidic platform that enables complete single-cell analysis on a heterogeneous pool of cells. We combine this architecture with an associated design-automation and optimization framework, referred to as Co-Synthesis (CoSyn). The proposed framework employs real-time resource allocation to coordinate the progression of concurrent cell analysis. Simulation results show that CoSyn efficiently utilizes platform resources and outperforms baseline techniques.

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Microfluidic Impedance Flow Cytometry Enabling High-Throughput Single-Cell Electrical Property Characterization

Cited by 135 publications

References 129 publications

Constriction Channel Based Single-Cell Mechanical Property Characterization

Constriction Channel Based Single-Cell Mechanical Property Characterization

High accuracy particle analysis using sheathless microfluidic impedance cytometry

CoSyn: Efficient single-cell analysis using a hybrid microfluidic platform

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