Microfluidic Methods in Janus Particle Synthesis

Saqib, Muhammad; Tran, Phong A.; Ercan, Batur; Erdem, E. Yegan

doi:10.2147/ijn.s371579

Cited by 8 publications

(6 citation statements)

References 86 publications

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“…Microfluidic devices are suitable for fabricating numerous uniform droplets. − Microchannels that generate Janus-shaped droplets have been designed, − of which are flow-focusing devices. − In this study, we investigated whether a flow-focusing device provides a scalable, one-step, shape-controllable approach for preparing collagen-enriched HFGs (Figure ). The prepared HFGs were transplanted into nude mice, and their hair regeneration efficiencies were evaluated.…”

Section: Introductionmentioning

confidence: 99%

Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

Sugiyama,

Nanmo,

Nie

et al. 2024

ACS Biomater. Sci. Eng.

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Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flowfocusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen microbeads with distinct Janus structures. During the 3 days of culture, the collagen beads contracted owing to cellular traction forces, resulting in collagen-and cell-dense HFGs. The transplantation of HFGs into nude mice resulted in highly efficient de novo hair follicle regeneration. This method provides a scalable and robust tissue graft preparation approach for hair regeneration.

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Section: Introductionmentioning

confidence: 99%

Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

Sugiyama,

Nanmo,

Nie

et al. 2024

ACS Biomater. Sci. Eng.

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“…Several methods have been reported for the synthesis of Janus particles [20,21]. Categorized broadly, the synthesis techniques are of two types: batch wise synthesis techniques and micro uidic based techniques.…”

Section: Introductionmentioning

confidence: 99%

“…Although these batch wise synthesis techniques are promising, they have inherent disadvantages that limit their applicability, such as polydispersity, limited control over reactions conditions, and wasteful consumption of reagents. In contrast micro uidic-based techniques provide superior control over reaction conditions, narrow size distribution of synthesized particles and minimal consumption of reagents [21]. Moreover, it allows precise control over the reaction conditions and e cient transfer of heat into and out of the system due to the small size of the microreactor.…”

Section: Introductionmentioning

confidence: 99%

“…Droplets in a micro uidic system act as individual microreactors that can be used to perform reactions with higher e ciency as compared to non-droplet based (continuous) micro uidic systems [25][26][27]. For these reasons micro uidic droplet-based synthesis techniques have gained popularity not only for isotropic particles but also for Janus particle synthesis [21]. There are several micro uidic-based techniques for the synthesis of Janus particles reported in the literature that can be broadly classi ed into four categories namely co-owing streams [28][29][30][31], phase separation [32][33][34][35][36][37], and double-emulsion [38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

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Biocompatible Janus Microparticle Synthesis in a Microfluidic Device

Saqib,

Tufan,

Orsel

et al. 2024

Preprint

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Janus particles are popular in recent years due to their anisotropic physical and chemical properties. Even though there are several established synthesis methods for Janus particles, microfluidics-based methods are convenient and reliable due to low reagent consumption, monodispersity of the resultant particles and efficient control over reaction conditions. In this work a simple droplet-based microfluidic technique is utilized to synthesize magnetically anisotropic TiO2-Fe2O3 Janus microparticles. Two droplets containing reagents for Janus particle were merged by using an asymmetric device such that the resulting droplet contained the constituents within its two hemispheres distinct from each other. The synthesized Janus particles were observed under the optical microscope and the scanning electron microscope. Moreover, a detailed in vitro characterization of these particles was completed, and it was shown that these particles have a potential use for biomedical applications.

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“…These distinctive properties of microfluidic reactors make them an attractive method for conducting controlled chemical reactions. Previously, the production of hybrid micro/nano particles within microreactors were reported in literature [ 21 , 33 , 36 , 39 – 42 ]. On the other hand, the synthesis of POSS particles is a relatively recent aspect in this field, and nearly all reported research on their synthesis has employed batch techniques.…”

Section: Introductionmentioning

confidence: 99%

Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor

Kibar,

Şahinoğlu,

Kılınçlı

et al. 2024

Microchim Acta

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This study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 $$\upmu $$ μ M. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation.

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Microfluidic Methods in Janus Particle Synthesis

Cited by 8 publications

References 86 publications

Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device

Biocompatible Janus Microparticle Synthesis in a Microfluidic Device

Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor

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