Microfluidic Multiplexing in Bioanalyses

Araz, M.K.; Tentori, Augusto M.; Herr, Amy E.

doi:10.1177/2211068213491408

Cited by 31 publications

(24 citation statements)

References 120 publications

(173 reference statements)

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“…Multiplexing is mainly realized through three different approaches: (i) spatial separation of detection sites, by means of different spots or wells, (ii) regional separation using discrete regions of a channel network or electrode arrays, or (iii) the use of various labels (e.g., enzymes, redox molecules, beads, and dyes). In particular, optical and electrochemical detection techniques are employed for the signal readout 6 , 7 .…”

Section: Current Multiplexing Technologiesmentioning

confidence: 99%

“…On the other hand, the regional separation of different detection sites (e.g., diversely functionalized electrodes or channel areas) has been increasingly employed in recent xPOCT applications. Their main drawbacks are their limited multiplexing levels and possible cross-sensitivity between single detection sites through diffusion 6 , 9 , 10 .…”

Section: Current Multiplexing Technologiesmentioning

confidence: 99%

See 1 more Smart Citation

Multiplexed Point-of-Care Testing – xPOCT

Dincer

Bruch

Kling

et al. 2017

Trends in Biotechnology

427

283

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Multiplexed point-of-care testing (xPOCT), which is simultaneous on-site detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resource-limited settings (such as in the developing world, in doctors’ offices, or directly at home). Nevertheless, only single-analyte approaches are typically considered as the major paradigm in many reviews of point-of-care testing. Here, we comprehensively review the present diagnostic systems and techniques for xPOCT applications. Different multiplexing technologies (e.g., bead- or array-based systems) are considered along with their detection methods (e.g., electrochemical or optical). We also address the unmet needs and challenges of xPOCT. Finally, we critically summarize the in-field applicability and the future perspectives of the presented approaches.

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Section: Current Multiplexing Technologiesmentioning

confidence: 99%

Section: Current Multiplexing Technologiesmentioning

confidence: 99%

Multiplexed Point-of-Care Testing – xPOCT

Dincer

Bruch

Kling

et al. 2017

Trends in Biotechnology

427

283

View full text Add to dashboard Cite

show abstract

“…Multiplexing is mainly achieved using spatial separation of targets, regional separation by targeting specific sites, or label-based techniques [53]. Though spatial and regional separation methods are highly employed for on-site multiplexed detection of various targets, they still suffer from expensive apparatus and complicated procedures [54,55]. An alternative method is target-labeling approach, which uses different molecular recognition elements (dyes, enzymes, DNA probes, beads, aptamers, etc.)…”

Section: Multiplex Sda Reactionmentioning

confidence: 99%

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

Zeng¹,

Mukama²,

Lu³

et al. 2019

Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies

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The identification of various targets such as bacteria, viruses, and other cells remains a prerequisite for point-of-care diagnostics and biotechnological applications. Nucleic acids, as encoding information for all forms of life, are excellent biomarkers for detecting pathogens, hereditary diseases, and cancers. To date, many techniques have been developed to detect nucleic acids. However, most of them are based on polymerase chain reaction (PCR) technology. These methods are sensitive and robust, but they require expensive instruments and trained personnel. DNA strand displacement amplification is carried out under isothermal conditions and therefore does not need expensive instruments. It is simple, fast, sensitive, specific, and inexpensive. In this chapter, we introduce the principles, methods, and updated applications of DNA strand displacement technology in the detection of infectious diseases. We also discuss how robust, sensitive, and specific nucleic acid detection could be obtained when combined with the novel CRISPR/Cas system.

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“…Therefore, they usually involve small sample volumes (µl, nl, pl, fl), a small size (submillimetre channels or capillaries), reduced energy consumption and a controlled microenvironment. 15 Current applications cover several scientific and commercial areas, including screening conditions for protein crystallisation, 16 high-throughput screening in drug development, 17 bioanalysis, 18 single cell analysis 19 and chemical synthesis, 20,21 to name a few.…”

Section: Advantages and Limitations Of Current Microfluidics Technologymentioning

confidence: 99%

A critical insight into the development pipeline of microfluidic immunoassay devices for the sensitive quantitation of protein biomarkers at the point of care

Barbosa

Reis

2017

Analyst

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The latest clinical procedures for the timely and cost-effective diagnosis of chronic and acute clinical conditions, such as cardiovascular diseases, cancer, chronic respiratory diseases, diabetes or sepsis (i.e. the biggest causes of death worldwide), involve the quantitation of specific protein biomarkers released into the blood stream or other physiological fluids (e.g. urine or saliva). The clinical thresholds are usually in the femtomolar to picolomar range, and consequently the measurement of these protein biomarkers heavily relies on highly sophisticated, bulky and automated equipment in centralised pathology laboratories. The first microfluidic devices capable of measuring protein biomarkers in miniaturised immunoassays were presented nearly two decades ago and promised to revolutionise point-of-care (POC) testing by offering unmatched sensitivity and automation in a compact POC format; however, the development and adoption of microfluidic protein biomarker tests has fallen behind expectations. This review presents a detailed critical overview into the pipeline of microfluidic devices developed in the period 2005-2016 capable of measuring protein biomarkers from the pM to fM range in formats compatible with POC testing, with a particular focus on the use of affordable microfluidic materials and compact low-cost signal interrogation. The integration of these two important features (essential unique selling points for the successful microfluidic diagnostic products) has been missed in previous review articles and explain the poor adoption of microfluidic technologies in this field. Most current miniaturised devices compromise either on the affordability, compactness and/or performance of the test, making current tests unsuitable for the POC measurement of protein biomarkers. Seven core technical areas, including (i) the selected strategy for antibody immobilisation, (ii) the surface area and surface-area-to-volume ratio, (iii) surface passivation, (iv) the biological matrix interference, (v) fluid control, (vi) the signal detection modes and (vii) the affordability of the manufacturing process and detection system, were identified as the key to the effective development of a sensitive and affordable microfluidic protein biomarker POC test.

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Microfluidic Multiplexing in Bioanalyses

Cited by 31 publications

References 120 publications

Multiplexed Point-of-Care Testing – xPOCT

Multiplexed Point-of-Care Testing – xPOCT

Strand Displacement Amplification for Multiplex Detection of Nucleic Acids

A critical insight into the development pipeline of microfluidic immunoassay devices for the sensitive quantitation of protein biomarkers at the point of care

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