Microfluidic nanodevices for drug sensing and screening applications

Pal, Arnab; Kaswan, Kuldeep; Barman, Snigdha Roy; Lin, Yu-Zih; Chung, Jun-Hsuan; Sharma, Manish Kumar; Liu, Kuei-Lin; Chen, Bo-Huan; Wu, Chih‐Cheng; Lee, Sang‐Min; Choi, Dongwhi; Lin, Zong‐Hong

doi:10.1016/j.bios.2022.114783

Cited by 26 publications

(8 citation statements)

References 201 publications

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“…A custom-made ECD cell was used to load and measure the nSLAM products (Supporting Information, Figure S16), which enabled the deposition of a CSNP-amplicon network on the surface of the detection substrate for precise measurements. ECD parameters, including CSNP load, voltammetric method, and applied voltage range, were optimized for high-sensitivity detection of samples to obtain maximum reproducible signals with minimal noise (Supporting Information, Figure S17 and Note S5). − …”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes

Park,

Soh,

Choi

et al. 2024

ACS Nano

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Circulating tumor DNA (ctDNA) detection has been acknowledged as a promising liquid biopsy approach for cancer diagnosis, with various ctDNA assays used for early detection and treatment monitoring. Dispersible magnetic nanoparticle-based electrochemical detection methods have been proposed as promising candidates for ctDNA detection based on the detection performance and features of the platform material. This study proposes a nanoparticle surface-localized genetic amplification approach by integrating Fe3O4–Au core–shell nanoparticles into polymerase chain reactions (PCR). These highly dispersible and magnetically responsive superparamagnetic nanoparticles act as nano-electrodes that amplify and accumulate target ctDNA in situ on the nanoparticle surface upon PCR amplification. These nanoparticles are subsequently captured and subjected to repetitive electrochemical measurements to induce reconfiguration-mediated signal amplification for ultrasensitive (∼3 aM) and rapid (∼7 min) metastatic breast cancer ctDNA detection in vitro. The detection platform can also detect metastatic biomarkers from in vivo samples, highlighting the potential for clinical applications and further expansion to rapid and ultrasensitive multiplex detection of various cancers.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes

Park,

Soh,

Choi

et al. 2024

ACS Nano

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“…In the last decade, there has been significant progress in multidisciplinary research on manufacturing, microelectronics, and their application in the life sciences (Zhou et al, 2023). These have had a remarkable impact on OoC development since the design flexibility of microfluidic chips can now be further complemented with analytical methods capable of probing small sample volumes or even single-cell information (Pal et al, 2023). The integration of a multitude of detection devices, such as electrodes and optics, in microfluidic chips allows time-resolved analysis of environmental factors for accurately monitoring cell culture state.…”

Section: Resolving High-content Data From Multisensors and Imaging Mo...mentioning

confidence: 99%

Guiding organs-on-chips towards applications: a balancing act between integration of advanced technologies and standardization

Meneses,

Conceição,

van der Meer

et al. 2024

Front. Lab. Chip. Technol.

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Organs-on-chips (OoC) are in vitro models that emulate key functionalities of tissues or organs in a miniaturized and highly controlled manner. Due to their high versatility, OoC have evolved as promising alternatives to animal testing for a more effective drug development pipeline. Additionally, OoC are revealing increased predictive power for toxicity screening applications as well as (patho-) physiology research models. It is anticipated that enabling technologies such as biofabrication, multimodality imaging, and artificial intelligence will play a critical role in the development of the next generation of OoC. These domains are expected to increase the mimicry of the human micro-physiology and functionality, enhance screening of cellular events, and generate high-content data for improved prediction. Although exponentially growing, the OoC field will strongly benefit from standardized tools to upgrade its implementational power. The complexity derived from the integration of multiple technologies and the current absence of concrete guidelines for establishing standards may be the reason for the slower adoption of OoC by industry, despite the fast progress of the field. Therefore, we argue that it is essential to consider standardization early on when using new enabling technologies, and we provide examples to illustrate how to maintain a focus on technology standards as these new technologies are used to build innovative OoC applications. Moreover, we stress the importance of informed design, use, and analysis decisions. Finally, we argue that this early focus on standards in innovation for OoC will facilitate their implementation.

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“…It is essential to establish human-relevant systems in vitro to develop effective antiviral drugs and vaccines, as many candidate antiviral drugs work in animals but not humans due to interspecies divergence [ [129] , [130] , [131] ]. Therefore, microfluidic-based models provide a new way to accurately predict therapeutic drug effects at the human organ level utilizing humanized living cells to model in-vivo-like organ development [ 132 ]. Lung chips and body-on-chips can provide bioengineering means to expedite the discovery and preclinical trials of therapeutic agents for COVID-19 and vaccines for SARS-CoV2, overcoming traditional animal models and 2D monolayer cultures [ 133 ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Microfluidic strategies for biomimetic lung chip establishment and SARS-CoV2 study

Wang,

Wen,

Zhu

et al. 2024

Materials Today Bio

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Microfluidic nanodevices for drug sensing and screening applications

Cited by 26 publications

References 201 publications

Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes

Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes

Guiding organs-on-chips towards applications: a balancing act between integration of advanced technologies and standardization

Microfluidic strategies for biomimetic lung chip establishment and SARS-CoV2 study

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