Microfluidic oxygen sensor system as a tool to monitor the metabolism of mammalian cells

Bunge, Frank; Driesche, Sander van den; Waespy, Mario; Radtke, Arlo; Belge, Gazanfer; Kelm, Sørge; Waite, Anya M.; Mirastschijski, Ursula; Vellekoop, Michael J.

doi:10.1016/j.snb.2019.03.041

Cited by 15 publications

(16 citation statements)

References 33 publications

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“…Since our gut–liver chip did not integrate with the dissolved oxygen sensor, we assumed that the oxygen level of the cell culture medium in the microchannel of the chip was approximately 20%, based on the gas‐permeable character of PDMS. The assumption was reasonable when we compared the assumption with that of other related studies 73,74 . Various concentrations of PA induced oxidative stress for HepG2 cells, as exhibited by fluorescent signals by DCF‐DA staining (Figure 6a,b).…”

Section: Resultssupporting

confidence: 55%

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In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip

Jeon

Lee

Kim

et al. 2021

Biotechnology Progress

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Hepatic steatosis, also known as fatty liver disease, occurs due to abnormal lipid accumulation in the liver. It has been known that gut absorption also plays an important role in the mechanism underlying hepatic steatosis. Conventional in vitro cell culture models have limitations in recapitulating the mechanisms of hepatic steatosis because it does not include the gut absorption process. Previously, we reported development of a microfluidic gut-liver chip that can recapitulate the gut absorption of fatty acids and subsequent lipid accumulation in liver cells. In this study, we performed a series of experiments to verify that our gut-liver chip reproduces various aspects of hepatic steatosis. The absorption of fatty acids was evaluated under various culture conditions. The anti-steatotic effect of turofexorate isopropyl (XL-335) and metformin was tested, and both drugs showed different action mechanisms. In addition, the oxidative stress induced by lipid absorption was evaluated. Our results demonstrate the potential of the gut-liver chip for use as a novel, physiologically realistic in vitro model to study fatty liver disease. K E Y W O R D Sfatty liver disease, gut-liver-on-a-chip, hepatic steatosis, multi-organ-on-a-chip, organ-ona-chip | INTRODUCTIONHepatic steatosis involves excessive accumulation of lipids in the liver due to alcohol abuse or obesity and is also called fatty liver disease. 1 Chronic hepatic steatosis can develop into steatohepatitis, which is characterized by hepatic necrosis and inflammation, and eventually progress into hepatic fibrosis and liver cancer. 2 As the incidence of hepatic steatosis has increased, the importance of treatment has likewise increased. 3 In terms of the pathogenesis of fatty liver disease, oxidative stress and metabolic diseases, such as insulin resistance, type 2 diabetes, and obesity, were found to be major factors. 2,4 However, more in-depth studies are required to fully understand the mechanism of hepatic steatosis and to develop therapeutic drugs, as various factors affect the progression of hepatic steatosis. 5 In previous studies, tissue biopsies and animal models have been used for studying hepatic steatosis. 6,7 However, tissue biopsies require surgical procedures to obtain samples from patients, and partial biopsies cause significant errors. 7 In addition, animal models are not suitable for the study of hepatic steatosis, as their histological changes differ from those observed in humans. 6 in vitro models of hepatic steatosis is also available, where human primary hepatocytes or cell lines exposed to fatty acids to induce lipid accumulation. [8][9][10] Although conventional in vitro models are easily accessible, they possess limitations in reproducing the in vivo environments, such as dynamic supplement of nutrients and oxygen, three-dimensional (3D) microenvironment, and multi-organ interactions, and thereby render it challenging to understand the underlying mechanisms of the disease. 11,12 Recently, organ-on-a-chip technology has gained attention as n...

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Section: Resultssupporting

confidence: 55%

“…The assumption was reasonable when we compared the assumption with that of other related studies. 73,74 cells in a static, in vitro environment ranges from 1000 to 2000 μm. 43,44,46 However, in animals and humans, the UWL thickness is less than 100 μm due to fluidic shear resulting from the intestinal peristalsis.…”

Section: Induction Of Oxidative Stress By Pamentioning

confidence: 99%

In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip

Jeon

Lee

Kim

et al. 2021

Biotechnology Progress

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“…On the other hand, optical microsystems require more complex manufacturing techniques [6]. The main issue is optical transparency-therefore silicon is commonly bonded with glass [7] and ceramics with PDMS (polydimethylsiloxane, an elastic silicon-based polymer [8]. Also, glass-PDMS systems are very popular due to reasonable manufacturing cost [9].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Microfluidic Sensor for Fluorescence Measurements

Nawrot

Gzubicka

Malecha

2020

Period. Polytech. Elec. Eng. Comp. Sci.

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In this work a fluorescent microfluidic sensor is developed, manufactured and characterized through additive manufacturing technique, using transparent photopolymer. A technology widely known as 3D printing is very suitable for tailor-made microfluidic chips, as it allows for rapid manufacturing. Optical structures can be developed using transparent materials, and this work describes a technique for increasing light transmission through such structure. A complete, inexpensive electronic device is described, containing microfluidic chip, light source, photodetector, microcontroller, communication interface and a multifunctional package with embedded connectors. Performed analyses show that performed surface modification significantly improves light transmission through structure, the material does not show autofluorescence, and whole device is suitable for fluorescence measurements.

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“…The use of integrated sensors is an engaging tool for diagnosis due to the fact of their high sensitivity, short analysis time, cost-effectiveness, and potential of miniaturization [34,35,36,37]. In particular, oxygen sensors have been used to monitor the respirometry or uptake of oxygen by mammalian cells in biolabs, as a direct indicator for cell metabolism [38].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Buenge et al developed a microfluidic chip to determine the oxygen consumption rate of cells by measuring the concentration of dissolved oxygen. The microfluidics chamber was composed of a 200 μm × 2.5 mm × 12.3 mm chamber, and the chip contained an electric heater and a temperature sensor on the bottom [38].…”

Section: Introductionmentioning

confidence: 99%

Portable and Low-Cost Respirometric Microsystem for the Static and Dynamic Respirometry Monitoring of Compost

Bermúdez

Saldarriaga

Osma

2019

Sensors

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Composting is considered an option for the disposal of organic waste; however, the development of portable and low-cost systems for its monitoring is of high interest. Therefore, in this study, respirometric microsystems were designed and tested including two integrated oxygen sensors for the measurement of compost samples under static and dynamic conditions with high portability and ease of use. The cost of each sensor was calculated as 2 USD, while the cost of the whole respirometric microsystem was calculated as 6 USD. The electronic system for real-time monitoring was also designed and implemented. The designed systems were tested for over 6 weeks for the determination of compost quality using real samples. The respirometric microsystem was compared to a commercial respirometry system and a standard laboratory test using hierarchical analysis which included costs, portability accuracy, analysis time, and integration of new technologies. The analysis showed a global score of 6.87 for the respirometric microsystem compared to 6.70 for the standard laboratory test and 3.26 for the commercial system.

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Microfluidic oxygen sensor system as a tool to monitor the metabolism of mammalian cells

Cited by 15 publications

References 33 publications

In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip

In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip

3D-Printed Microfluidic Sensor for Fluorescence Measurements

Portable and Low-Cost Respirometric Microsystem for the Static and Dynamic Respirometry Monitoring of Compost

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