Microfluidic Platform for Analyzing the Thermotaxis of C. elegans in a Linear Temperature Gradient

Yoon, Sunhee; Piao, Hailing; Jeon, Tae‐Joon; Kim, Sun Min

doi:10.2116/analsci.33.1435

Cited by 4 publications

(4 citation statements)

References 33 publications

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“…The thermotaxis of C. elegans depends mainly on the culture temperature gradient. The microfluidic system developed by Yoon et al [ 107 ], which consists of a linear microchannel and Peltier modules, can precisely control temperature gradients (Fig. 4 E).…”

Section: Microfluidic Sorting Of C Elegans and Dev...mentioning

confidence: 99%

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Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Yuan

Duan

et al. 2023

Cyborg Bionic Syst

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Caenorhabditis elegans ( C. elegans ) has been a popular model organism for several decades since its first discovery of the huge research potential for modeling human diseases and genetics. Sorting is an important means of providing stage- or age-synchronized worm populations for many worm-based bioassays. However, conventional manual techniques for C. elegans sorting are tedious and inefficient, and commercial complex object parametric analyzer and sorter is too expensive and bulky for most laboratories. Recently, the development of lab-on-a-chip (microfluidics) technology has greatly facilitated C. elegans studies where large numbers of synchronized worm populations are required and advances of new designs, mechanisms, and automation algorithms. Most previous reviews have focused on the development of microfluidic devices but lacked the summaries and discussion of the biological research demands of C. elegans , and are hard to read for worm researchers. We aim to comprehensively review the up-to-date microfluidic-assisted C. elegans sorting developments from several angles to suit different background researchers, i.e., biologists and engineers. First, we highlighted the microfluidic C. elegans sorting devices' advantages and limitations compared to the conventional commercialized worm sorting tools. Second, to benefit the engineers, we reviewed the current devices from the perspectives of active or passive sorting, sorting strategies, target populations, and sorting criteria. Third, to benefit the biologists, we reviewed the contributions of sorting to biological research. We expect, by providing this comprehensive review, that each researcher from this multidisciplinary community can effectively find the needed information and, in turn, facilitate future research.

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Section: Microfluidic Sorting Of C Elegans and Dev...mentioning

confidence: 99%

Section: Microfluidic Sorting Of C Elegans and Dev...mentioning

confidence: 99%

Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Yuan

Duan

et al. 2023

Cyborg Bionic Syst

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“…Furthermore, advanced active droplet manipulation tools [46][47][48][49] are inadequate as mechanical, thermal, and electrical-induced forces may create undesired stimulation. [50][51][52] Addressing these limitations is critical to fully exploit the potential of multiphase microfluidics as smallanimal screening technology.Here we describe a multiphase microfluidic platform to screen dynamic phenotypes in small animals. We demonstrate the use of this platform with the nematode C. elegans, Screening functional phenotypes in small animals is important for genetics and drug discovery.…”

mentioning

confidence: 99%

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confidence: 99%

Automated and Dynamic Control of Chemical Content in Droplets for Scalable Screens of Small Animals

Aubry

Milisavljevic

2022

Small

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the limited ability to change the environment and 3D animal motion make it difficult to assess dynamic phenotypes other than rough motion analysis. In contrast, microfluidics, with relevant length scales down to the animal's size, offers improved means to handle small animals and control their environment. [10][11][12][13][14] Animals can be confined in 2D or even immobilized, and liquids can be exchanged rapidly. These approaches enable advanced functional assays such as monitoring brain activity [15,16] or performing high-resolution behavior. [17] However, most of the highthroughput screening techniques are end-point assays based on fluorescence images [18,19] and do not translate well for phenotyping based on functional imaging. Single-phase microfluidic systems, although powerful, have limitations in the number of animals they can handle [17,[20][21][22][23] and the ability to modulate the experimental conditions at will. [16,[23][24][25] Multiphase microfluidics has unique attributes that make it well suited to tackle these challenges. These include the ability to partition, create independent containers of various sizes, generate precise chemical environments, perform serial processing, and consume ultra-low amounts of reagents. Multiphase microfluidics has led to advanced screening technologies impacting a broad range of disciplines, from particle synthesis [26,27] and synthetic biology, [28,29] to drug discovery, [30,31] and research in fundamental biology. [32,33] It has been applied successfully for numerous single-cell screens [34] and is gaining popularity for multicellular organisms such as spheroids and organoids. [35][36][37][38] In contrast, the development of multiphase microfluidics techniques for small animals [39][40][41][42][43] has seen a slow progress because of several technical bottlenecks. For instance, passive encapsulation techniques [44] are not adaptable to motile, multi-cellular organisms, mining the throughput. Second, techniques for controlling the droplet composition post-production rely mainly on merging, [45] which limits the ability to dynamically alter the droplet content. Furthermore, advanced active droplet manipulation tools [46][47][48][49] are inadequate as mechanical, thermal, and electrical-induced forces may create undesired stimulation. [50][51][52] Addressing these limitations is critical to fully exploit the potential of multiphase microfluidics as smallanimal screening technology.Here we describe a multiphase microfluidic platform to screen dynamic phenotypes in small animals. We demonstrate the use of this platform with the nematode C. elegans, Screening functional phenotypes in small animals is important for genetics and drug discovery. Multiphase microfluidics has great potential for enhancing throughput but has been hampered by inefficient animal encapsulation and limited control over the animal's environment in droplets. Here, a highly efficient single-animal encapsulation unit, a liquid exchanger system for controlling the droplet chemical environment dynami...

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Micro systems for the study of behavioral responses of C. elegans to various physical and chemical stimuli

Yoon

Fuwad

Jeon

et al. 2021

Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms

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Microfluidic Platform for Analyzing the Thermotaxis of C. elegans in a Linear Temperature Gradient

Cited by 4 publications

References 33 publications

Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Microfluidic-Assisted Caenorhabditis elegans Sorting: Current Status and Future Prospects

Automated and Dynamic Control of Chemical Content in Droplets for Scalable Screens of Small Animals

Micro systems for the study of behavioral responses of C. elegans to various physical and chemical stimuli

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