Microfluidic Preparation of Liposomes to Determine Particle Size Influence on Cellular Uptake Mechanisms

Andar, Abhay; Hood, Renee R.; Vreeland, Wyatt N.; DeVoe, Don L.; Swaan, Peter W.

doi:10.1007/s11095-013-1171-8

Cited by 136 publications

(97 citation statements)

References 39 publications

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“…9) and to visualize the morphology changes of zwitterionic vesicles upon their complexations with DNA. Size distributions of the formed complexes were verified by these three methods; however, the observed trends have also functional implications, since the effect of size and surface charges is considered determining factors in cell transfections (Moghaddam et al 2011;Andar et al 2014). In this respect, it would be interesting to explore in future studies the role of lipoplex size on cellular uptake mechanisms emphasizing the importance of increased target cell type specificity, increased transfection efficacy and reduced nanoparticle cytotoxicity of the delivery vectors (Lewinski et al 2008).…”

Section: Fluorescence and Uv/vis Spectroscopymentioning

confidence: 86%

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

2015

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The basic interfacial characteristics of DNA-lipid recognitions have been studied. The complex structures of individual unbound DNA molecules and their binary and ternary complexes with zwitterionic lipids and divalent cations were followed by employing lipid monolayers at the air-liquid interfaces, as well as by performing various microscopic, spectroscopic, and thermodynamic measurements with multilayered vesicles. The pressure-area isotherms depicted that Mg 2? -ions increase the surface pressure of lipid films and thus give rise to electrostatic and hydrophobic lipid-DNA interactions in terms of DNA adsorption, adhesion, and compaction. These features were further approached by using multilamellar vesicles with a mean diameter of 850 nm, where a metal ion-directed nucleic acid compaction and condensation effects were shown. The data obtained show the effectiveness of Langmuir monolayers and lipid multilayers in studying nucleic acid-lipid recognitions. The data provide with further details and support previous reports on mainly structural features of these recognitions. Biomolecular surface recognition events were presented in direct link with spectral and thermodynamic features of lipid vesicle-polynucleotide complex formations. The results serve to build a theoretical model considering the use of neutral lipids in lipoplex designs as a polyelectrolyte alternatives to the currently employed cytotoxic cationic liposomes. The supramolecular structures formed and their possible roles in interfacial electrostatic and hydrophobic mechanisms of endosomal escape in relevant cell transfection assays are particularly emphasized.

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Section: Fluorescence and Uv/vis Spectroscopymentioning

confidence: 86%

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

2015

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“…In fact it is well known that the size and charge of nanoparticles has a profound effect on their (in vivo/ ex-vivo) intestinal transport (Araujo et al, 2014;Jani et al, 1990Jani et al, , 1989. Furthermore, the potential of microfluidics to formulate liposomes with very narrow size distributions was recently used to prepare liposomes with sizes from 40 nm up to 276 nm, which were then found to have different uptake by Caco-2 cells; as liposome size increased their cell-uptake decreased, while differences in the mechanisms of uptake of the liposome with different sizes were additionally found (Andar et al, 2014). In fact, it would be interesting in the future to investigate if the currently demonstrated effects of liposome size on their interaction with the hCMEC/D3 cellular model of the BBB are also related to different mechanisms of uptake/translocation.…”

Section: Discussionmentioning

confidence: 99%

How do the physicochemical properties of nanoliposomes affect their interactions with the hCMEC/D3 cellular model of the BBB?

Papadia

Markoutsa

Antimisiaris

2016

International Journal of Pharmaceutics

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“…29 The liposomal characters are shown in Table 1. The particle sizes of the five liposomes were less …”

Section: Pharmaceutical Characters Of Liposomesmentioning

confidence: 99%

Hepatocellular carcinoma-targeted effect of configurations and groups of glycyrrhetinic acid by evaluation of its derivative-modified liposomes

Sun

Dai

Yin

et al. 2018

IJN

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Background There are abundant glycyrrhetinic acid (GA) receptors on the cellular membrane of hepatocytes and hepatocellular carcinoma (HCC) cells. The receptor binding effect might be related to the structure of the guiding molecule. GA exists in two stereoisomers with C3-hydroxyl and C11-carbonyl active groups. Purpose The objective of this study was to investigate the relationship between the HCC-targeted effect and the configurations and groups of GA. Methods and results Different GA derivatives (18β-GA, 18α-GA, 3-acetyl-18β-GA [3-Ace-GA] and 11-deoxy-18β-GA [11-Deo-GA]) were used to investigate the targeting effect of GA’s configurations and groups on HCC cells. The EC 50 values of competition to binding sites and the ratio of specific binding in HepG2 cells showed that 18β-GA and 3-Ace-GA demonstrated significant competitive effect with fluorescein isothiocyanate (FITC)-labeled GA. Then, the GA derivatives were distearoyl-phosphatidylethanolamine (DSPE)-PEGylated. 18β-GA-, 18α-GA-, 3-Ace-GA-and 11-Deo-GA-modified liposomes were prepared and characterized by size, zeta potential, encapsulation efficiency, loading capacity, leakage and membrane stability. Evaluation on the cellular location in vitro and tumor targeting in vivo was carried out. Compared to common long-circulation liposome (PEG-Lip), more 18β-GA- and 3-Ace-GA-modified liposomes aggregated around HepG2 cells in vitro in short time and transferred into HCC tumors in vivo for a longer time. Conclusion The β-configuration hydrogen atom on C18 position of GA played the most important role on the targeting effect. C11-carbonyl and C3-hydroxy groups of GA have certain and little influence on targeting action to HCC, respectively. In general, GA might be a promising targeting molecule for the research on liver diseases and hepatoma therapy.

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Microfluidic Preparation of Liposomes to Determine Particle Size Influence on Cellular Uptake Mechanisms

Cited by 136 publications

References 39 publications

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

Supramolecular Langmuir monolayers and multilayered vesicles of self-assembling DNA–lipid surface structures and their further implications in polyelectrolyte-based cell transfections

How do the physicochemical properties of nanoliposomes affect their interactions with the hCMEC/D3 cellular model of the BBB?

Hepatocellular carcinoma-targeted effect of configurations and groups of glycyrrhetinic acid by evaluation of its derivative-modified liposomes

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