Microfluidic Single-Cell Analytics

Microbial lifelines in bioprocesses: From concept to application

“…Detection of the resulting intracellular lifelines needs to be non-(or minimally) -invasive to ensure the individual microbe's vitality for further tracking in time. Microscopy, especially live-cell imaging, is the most essential technique for microfluidic live-cell imaging, which can yield data on cell count, morphometrics like cell length and area, as well as cell mass (Dusny, 2020). With quantitative phase microscopy and the exploitation of the cell's refractive index, even the biomass of a single cell can be estimated (Popescu et al, 2008).…”

“…In this way, the intracellular concentration of products (Binder et al, 2012) and metabolites acting as indicator for the flux through a pathway (Monteiro et al, 2019) can be monitored. A drawback of the application of translation dependent biosensors is, that the lag-time between sensing and expression needs to be considered as well as protein degradation dynamics to also decrease the output signal in an appropriate timeframe (Dusny, 2020). Alternatively, fluorescent protein biosensors that change their intensity (intensiometric) or the excitation or emission spectrum (ratiometric) based on the concentration of a target can react to changes in real-time (Bolbat and Schultz, 2017).…”

Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum

“…Microfluidic single-cell cultivation is a technique to analyze single cells in highly controlled environments, enabling the observation of population heterogeneity and single cell reactions to stressors. 2 The protocol below describes the wet-lab procedure to design, fabricate and apply the dynamic microfluidic single-cell cultivation (dMSCC) system for Corynebacterium glutamicum in rapidly changing environments. The bacteria grow in monolayer growth chambers (MGCs), which are connected to supply channels.…”

Growth and eGFP Production of CHO-K1 Suspension Cells Cultivated From Single Cell to Laboratory Scale

“…Prospectively, the integration of novel analytical procedures like single-cell mass spectrometry will make kinetics of biomass and product formation as well as substrate uptake and consumption are determinable (Dusny, 2020).…”