Microfluidic sorting of arbitrary cells with dynamic optical tweezers

Landenberger, Benjamin; Höfemann, Henning; Wadle, Simon; Rohrbach, Alexander

doi:10.1039/c2lc21099a

Cited by 106 publications

(48 citation statements)

References 25 publications

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“…6354 cells/min [72] and 1320 cells/min [67] has been for deflection-based sorters. Grabbing-based sorters are almost an order of magnitude slower with reported values of 300 cells/min [73] and 84 cells/min [74] (the throughput of several OP application can be seen in Fig. 6).…”

Section: Flow-rate and Throughputmentioning

confidence: 99%

“…Optical forces can also be utilized for sorting in a microfluidic environment by optically deflecting fluorescently labeled cells with a throughput of ∼20-100 cells/s [72]. Optical tweezer based microfluidic sorters have been able to isolate infected mouse macrophages from a population to show its effective use in diagnostics [67], and cells with different sizes using adaptive image processing methods [74,73]. OP methods have been worked extensively, and following the research phase, commercialization of microfluidic based optical trapping technology is underway [149].…”

Section: Applicationmentioning

confidence: 99%

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Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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Section: Flow-rate and Throughputmentioning

confidence: 99%

Section: Applicationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

View full text Add to dashboard Cite

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“…6(a)). 83 Cells not grabbed by the optical tweezers stream into a different reservoir than those actively displaced to a parallel streamline. The system is preferably suitable for small populations of a few hundred to thousand cells.…”

Section: Optical Tweezersmentioning

confidence: 99%

“…When a laser beam is focused on a particle or cell, it forms a three dimensional optical potential well that can induce optical pressure to capture particle. The captured particles or cells can be transported for sorting [81][82][83][84] or patterning 85,86 by controlling the track of optical tweezers. The sorting methods based on optical tweezers can be classified into passive and active modes.…”

Section: Optical Tweezersmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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“…[6][7][8][9][10] First, the particles injected by the flow into a microchip are focused or concentrated positionally in the focusing segment, and then separated based on their size or properties by a force against or across the flow induced by multiple streams, such as designed structures of the microchannel and applied fields in the separation segment. Finally, they are individually collected from two or more outlets at the fractionation segment.…”

Section: Introductionmentioning

confidence: 99%

Staggered-electromagnetophoresis with a Split-flow System for the Separation of Microparticles by a Hollow Fiber-embedded PDMS Microchip

et al. 2016

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Recently, we have developed a new microchip separation method for microparticles utilizing electromagnetophoresis (EMP) under the concept of the free-flow technique. 27 EMP, which was the migration phenomenon based on the Lorentz force proposed for the first time by Kolin in 1953, 28 is a force induced on a particle placed in a magnetic and electric field 2016 © The Japan Society for Analytical Chemistry † To whom correspondence should be addressed. A novel microchip separation system for microparticles based on electromagnetophoresis (EMP) was developed. In this system, focusing and separation of flowing microparticles in a microchannel could be performed by staggered-EMP by controlling the electric current applied to the channel locally combined with the split-flow system for fractionation of eluates. To apply the electric current through the flushing medium in the microchannel, a hollow fiber-embedded microchip with multiple electrodes was fabricated. The hollow fiber was made by a semi-permeable membrane and could separate small molecules. This microchip allowed us to apply the electric current to a part of the microchannel without any pressure control device because a main channel contacted with the subchannels that had electrodes through the semi-permeable membrane. Moreover, the separation using this microchip was combined with the split-flow system at two outlets to improve separation efficiency. Using this system, with the split-flow ratio of 10:1, 87% of 3 μm polystyrene (PS) latex particles were isolated from a mixture of 3 and 10 μm particles. Even the separation of 6 and 10 μm PS particles was achieved with about 77% recovery and 100% purity. In addition, by controlling the applied current, size fractionation of polypropylene (PP) particles was demonstrated. Moreover, biological particles such as pollens could be separated with high separation efficiency by this technique.

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Microfluidic sorting of arbitrary cells with dynamic optical tweezers

Cited by 106 publications

References 25 publications

Microfluidic bio-particle manipulation for biotechnology

Microfluidic bio-particle manipulation for biotechnology

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Staggered-electromagnetophoresis with a Split-flow System for the Separation of Microparticles by a Hollow Fiber-embedded PDMS Microchip

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