Microfluidic surface-enhanced infrared spectroscopy with semiconductor plasmonics for the fingerprint region

Bomers, M.; Charlot, B.; Barho, Franziska; Chanuel, Antoine; Mezy, Aude; Cerutti, L.; Гонзалез-Посада, Ф.; Taliercio, Thierry

doi:10.1039/c9re00350a

Cited by 13 publications

(14 citation statements)

References 51 publications

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“…The emergence of metamaterials, which are artificial materials composed of metallodielectric nanostructures, has allowed a new degree of freedom to engineer hot-spots in order to improve the enhancement factor in SEIRA. Many micro/nanofluidic devices integrated with metamaterials were demonstrated for in-situ probing of biomolecule interactions through SEIRA [ 57 , 58 , 59 ]. SEIRA provides spectroscopic information complementary to SERS, and its signals are usually stronger than those in SERS; however, the difficulty in fabricating IR-compatible device limits its application in micro/nanofluidics.…”

Section: Optical Detectionmentioning

confidence: 99%

Advances in Label-Free Detections for Nanofluidic Analytical Devices

Shimizu

Morikawa

2020

Micromachines

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Nanofluidics, a discipline of science and engineering of fluids confined to structures at the 1–1000 nm scale, has experienced significant growth over the past decade. Nanofluidics have offered fascinating platforms for chemical and biological analyses by exploiting the unique characteristics of liquids and molecules confined in nanospaces; however, the difficulty to detect molecules in extremely small spaces hampers the practical applications of nanofluidic devices. Laser-induced fluorescence microscopy with single-molecule sensitivity has been so far a major detection method in nanofluidics, but issues arising from labeling and photobleaching limit its application. Recently, numerous label-free detection methods have been developed to identify and determine the number of molecules, as well as provide chemical, conformational, and kinetic information of molecules. This review focuses on label-free detection techniques designed for nanofluidics; these techniques are divided into two groups: optical and electrical/electrochemical detection methods. In this review, we discuss on the developed nanofluidic device architectures, elucidate the mechanisms by which the utilization of nanofluidics in manipulating molecules and controlling light–matter interactions enhances the capabilities of biological and chemical analyses, and highlight new research directions in the field of detections in nanofluidics.

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Section: Optical Detectionmentioning

confidence: 99%

Advances in Label-Free Detections for Nanofluidic Analytical Devices

Shimizu

Morikawa

2020

Micromachines

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“…Flow reaction products are most often analysed after the reaction mixture exits the reactor (for example, by HPLC, UV‐Vis, MS) but if narrow glass channels are used in the flow reactor this offers the possibility of intra‐chip analysis as the reaction proceeds. Infra‐red and nuclear magnetic resonance spectroscopies are methods that have been successfully applied to intra‐chip reaction monitoring [21,22] . To date, the use of fluorescence to monitor organic reactions as they proceed in flow remains relatively unexplored.…”

Section: Introductionmentioning

confidence: 99%

“…Infra-red and nuclear magnetic resonance spectroscopies are methods that have been successfully applied to intra-chip reaction monitoring. [21,22] To date, the use of fluorescence to monitor organic reactions as they proceed in flow remains relatively unexplored. While inflow fluorescence sensor quenching and lifetime reaction monitoring have been developed, to the best of our knowledge Chemistry-A European Journal intra-chip fluorescence intensity monitoring has not been applied to continuous flow organic reactions.…”

Section: Introductionmentioning

confidence: 99%

Continuous Flow Bioconjugations of NIR‐AZA Fluorophores via Strained Alkyne Cycloadditions with Intra‐Chip Fluorogenic Monitoring**

O’Shea

Fitzgerald

2022

Chemistry A European J

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The importance of bioconjugation reactions continues to grow for cell specific targeting and dual therapeutic plus diagnostic medical applications. This necessitates the development of new bioconjugation chemistries, in‐flow synthetic and analytical methods. With this goal, continuous flow bioconjugations were readily achieved with short residence times for strained alkyne substituted carbohydrate and therapeutic peptide biomolecules in reaction with azide and tetrazine substituted fluorophores. The strained alkyne substrates included substituted 2‐amino‐2‐deoxy‐α‐D‐glucopyranose, and the linear and cyclic peptide sequences QIRQQPRDPPTETLELEVSPDPAS‐OH and c(RGDfK) respectively. The catalyst and reagent‐free inverse electron demand tetrazine cycloadditions proved more favourable than the azide 1,3‐dipolar cycloadditions. Reaction completion was achieved with residence times of 5 min at 40 °C for tetrazine versus 10 min at 80 °C for azide cycloadditions. The use of a fluorogenic tetrazine fluorophore, in a glass channelled reactor chip, allowed for intra‐chip reaction monitoring by recording fluorescence intensities at various positions throughout the chip. As the Diels‐Alder reactions proceeded through the chip, the fluorescence intensity increased accordingly in real‐time. The application of continuous flow fluorogenic bioconjugations could offer an efficient translational access to theranostic agents.

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“…Infra-red and nuclear magnetic resonance spectroscopies are methods that have been successfully applied to intra-chip reaction monitoring. 21,22 To date, the use of fluorescence to monitor organic reactions as they proceed in flow remains unexplored. 23 Bioconjugation chemistries are near universally performed as relatively small-scale batch reactions under mild temperature conditions due to the sensitive nature of bio-molecules.…”

Section: Introductionmentioning

confidence: 99%

Continuous Flow Bioconjugations of NIR-AZA Fluorophores via Strained Alkyne Cycloadditions with Intra-chip Fluorogenic Monitoring

O’Shea

Fitzgerald

2021

Preprint

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The importance of bioconjugation reactions continues to grow as the need for cell specific targeting and dual therapeutic plus diagnostic medical applications increase. This necessitates new bioconjugation chemistries, synthetic and analytical methods. With this goal, continuous flow bioconjugations were readily achieved with short residence times for strained alkyne substituted carbohydrate and peptide biomolecules in reaction with azide and tetrazine substituted fluorophores. The catalyst and reagent-free inverse electron demand tetrazine cycloadditions proved more favourable than the azide 1,3-dipolar cycloadditions. The use of a fluorogenic tetrazine fluorophore in a glass channelled reactor chip allowed for intra-chip reaction monitoring by recording fluorescence intensities at various positions throughout the chip. As the Diels-Alder reactions proceeded through the chip, the fluorescence intensity increased accordingly in real-time. This novel approach to continuous flow bioconjugation reaction with monitoring may offer advantages over post-chip analysis.

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Microfluidic surface-enhanced infrared spectroscopy with semiconductor plasmonics for the fingerprint region

Abstract: III–V semiconductor plasmonics enables to perform microfluidic surface-enhanced mid-IR spectroscopy and to access the so-called molecular fingerprint region from 6.7 μm to 20 μm (1500–500 cm−1).

Cited by 13 publications

References 51 publications

Advances in Label-Free Detections for Nanofluidic Analytical Devices

Advances in Label-Free Detections for Nanofluidic Analytical Devices

Continuous Flow Bioconjugations of NIR‐AZA Fluorophores via Strained Alkyne Cycloadditions with Intra‐Chip Fluorogenic Monitoring**

Continuous Flow Bioconjugations of NIR-AZA Fluorophores via Strained Alkyne Cycloadditions with Intra-chip Fluorogenic Monitoring

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