Microfluidic Surface Plasmon Resonance Sensors: From Principles to Point-of-Care Applications

Abstract

Surface plasmon resonance (SPR) is a label-free, highly-sensitive, and real-time sensing technique. Conventional SPR sensors, which involve a planar thin gold film, have been widely exploited in biosensing; various miniaturized formats have been devised for portability purposes. Another type of SPR sensor which utilizes localized SPR (LSPR), is based on metal nanostructures with surface plasmon modes at the structural interface. The resonance condition is sensitive to the refractive index change of the local medium. The principles of these two types of SPR sensors are reviewed and their integration with microfluidic platforms is described. Further applications of microfluidic SPR sensors to point-of-care (POC) diagnostics are discussed.

New advances in microfluidic flow cytometry

“…There are many other optical‐based detection techniques used in microfluidic devices including chemiluminescence , TL detection , surface plasma resonance detection , and surface‐enhanced Raman scattering (SERS) detection . Although these techniques are sensitive, they are not suitable to be used in the microfluidic flow cytometry due to their detection mechanism.…”

A microfluidic in-line ELISA for measuring secreted protein under perfusion

“…This is in part attributable to the technical challenge of integrating hardware with microfluidics and the lack of sensitivity of the assays, especially, for the small volumes associated with microfluidics. Several analytical techniques including mass spectrometry, ion mobility spectrometry, electrochemical detection, capillary electrophoresis, and surface plasmon resonance have been used to detect cellular secretions (Chen, 2012, Wang, 2016, Wang, 2016). For example, cellular proteins have been measured using microfluidic capillary electrophoresis and mass spectrometry (Lomasney, 2013, Dugan, 2014, Wang, 2016) however, there are several general limitations.…”

“…Several analytical techniques including mass spectrometry, ion mobility spectrometry, electrochemical detection, capillary electrophoresis, and surface plasmon resonance have been used to detect cellular secretions (Chen, 2012, Wang, 2016, Wang, 2016). For example, cellular proteins have been measured using microfluidic capillary electrophoresis and mass spectrometry (Lomasney, 2013, Dugan, 2014, Wang, 2016) however, there are several general limitations. Although these methods are quite sensitive, sample introduction, integration and analysis are complex (Schultz, 1993, Tao, 1998, Lin, 2018) and except for the microfluidic electrophoresis, these analytical techniques lack the desired sensitivity for continuous analysis of cellular secretions in microfluidics.…”

Fabricación de un Biosensor de Celda Microfluídica para la Estandarización de Muestras de ADN Metilado en Individuos con Obesidad y Diabetes

“…Recientemente se han integrado dispositivos de microfluídica con plataformas de detección de plasmones superficiales (SPR). Las ventajas de esta integración van desde el empleo de volúmenes mínimos y procesamiento rápido, hasta el aumento de la eficiencia en la detección con diseños específicos y adecuados; como el aquí presentado [5,6].La técnica de resonancia de SPR aprovecha una propiedad óptica menos conocida de los metales, y es que bajo ciertas condiciones la luz puede viajar por las superficies metálicas sin alejarse de ellas. Esta es una propiedad muy peculiar, ya que en condiciones normales la luz viaja por las tres dimensiones del espacio y no se le confina con facilidad.…”

“…Recientemente se han integrado dispositivos de microfluídica con plataformas de detección de plasmones superficiales (SPR). Las ventajas de esta integración van desde el empleo de volúmenes mínimos y procesamiento rápido, hasta el aumento de la eficiencia en la detección con diseños específicos y adecuados; como el aquí presentado [5,6].…”