Microfluidic Systems for Assisted Reproductive Technologies: Advantages and Potential Applications

Sequeira, Russel C.; Criswell, Tracy; Atala, Anthony; Yoo, James J.

doi:10.1007/s13770-020-00311-2

Cited by 22 publications

(11 citation statements)

References 72 publications

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“…48 Microfluidic chips designed for use in ARTs are plentiful and have been subjected to a number of reviews in the past 5 years. [49][50][51][52][53][54][55][56][57][58][59][60][61] Due to the high compatibility of sperm for its manipulation within microstructures, a plethora of such chips solely for the collection, handling and analysis of semen have been proposed and discussed. [62][63][64][65][66][67][68][69][70][71] Examples of microfluidic chips developed for the in vitro processing of oocytes, 72,73 sperm cells 74,75 and the culture of embryos 76 are presented in Fig.…”

Section: Microfluidicsmentioning

confidence: 99%

Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs

et al. 2022

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Section: Microfluidicsmentioning

confidence: 99%

Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs

et al. 2022

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“…During human reproduction, even the preimplantation embryo, including the morula and, later on, the early blastocyst, is subject to fluidic flow forces generated by peristalsis of the fallopian tube [ 87 ]. The use of microfluidic devices in the culture, maintenance and study of ovarian follicle development for experimental and therapeutic applications, along with microfluidic platforms for oocyte and sperm selection and maintenance, facilitation of fertilization with in vitro fertilization or intracytoplasmic sperm injection, and monitoring, selection and maintenance of resulting embryos, are explained in [ 88 ].…”

Section: Microfluidics In Gametes Fertilizationmentioning

confidence: 99%

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

2021

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Infertility is a state of the male or female reproductive system that is defined as the failure to achieve pregnancy even after 12 or more months of regular unprotected sexual intercourse. Assisted reproductive technology (ART) plays a crucial role in addressing infertility. Various ART are now available for infertile couples. Fertilization in vitro (IVF), intracytoplasmic sperm injection (ICSI) and intrauterine insemination (IUI) are the most common techniques in this regard. Various microfluidic technologies can incorporate various ART procedures such as embryo and gamete (sperm and oocyte) analysis, sorting, manipulation, culture and monitoring. Hence, this review intends to summarize the current knowledge about the application of this approach towards cell biology to enhance ART.

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“…The development of high efficiency, automated cell transfection strategies has been a crucial requirement for applications ranging from assisted reproductive technology (e.g. IVF) [1] and the generation of transgenic organisms (e.g. C. elegans, drosophila, zebrafish) [2][3][4][5][6][7][8] to the optimization of genetic engineering technologies (e.g.…”

Section: Introductionmentioning

confidence: 99%

Micromechanical valve-operated needle-on-a-chip microinjection module for microfluidic large-scale integration

Gray-Scherr

Gasvoda²,

Hadsell³

et al. 2022

J. Micromech. Microeng.

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Microinjection is an essential process in genetic engineering that is used to deliver genetic materials into various biological specimens. Considering the high-throughput requirement for microinjection applications ranging from gene editing to cell therapies, there is a need for an automated, highly parallelized, reproducible, and easy-to-use microinjection strategy. Here we report an on-chip, microfluidic microinjection module designed for compatibility with microfluidic large-scale integration (mLSI) technology and that can be fabricated via standard, multilayer soft lithography techniques. The needle-on-chip (NOC) module consists of a two-layer PDMS-based microfluidic module whose puncture and injection operations are reliant solely on Quake valve actuation. We designed a NOC module to conduct the microinjection of a common genetics model organism, Caenorhabditis elegans (C. elegans). The NOC design was analyzed using finite element method simulations for a large range of practically viable geometrical parameters. The computational results suggested that a slight lateral offset (>10 μm) of the control channel is sufficient for a successful NOC operation with a large fabrication tolerance (50 μm, 50% channel width). To demonstrate proof-of-concept, the microinjection platform was fabricated and utilized to perform a successful injection of a tracer dye into C. elegans.

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Microfluidic Systems for Assisted Reproductive Technologies: Advantages and Potential Applications

Cited by 22 publications

References 72 publications

Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs

Electrically-driven handling of gametes and embryos: taking a step towards the future of ARTs

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

Micromechanical valve-operated needle-on-a-chip microinjection module for microfluidic large-scale integration

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