Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Fraser, Lewis A.; Cheung, Yiu-ming; Kinghorn, Andrew B.; Guo, Wenping; Shiu, Simon Chi‐Chin; Jinata, Chandra; Liu, Mengping; Bhuyan, Soubhagya Kumar; Nan, Lang; Shum, Ho Cheung; Tanner, Julian A.

doi:10.1002/adbi.201900012

Cited by 28 publications

(22 citation statements)

References 172 publications

(293 reference statements)

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“…RNAcleaving fluorogenic DNAzyme (RFD) is a wellestablished technology for detecting bacteria, and the ability to immobilize RFD probes on material surfaces such as the interiors of microflu idic devices is highly demanded because it could enable substantial improvements in the efficiency and speed of detection (51)(52)(53). Our genetically engineered CsgA fusion coatings represent a potentially alternative approach.…”

Section: Functional Csga Dbd Coating-enabled Microfluidic Bacterial Sensorsmentioning

confidence: 99%

Conformable self-assembling amyloid protein coatings with genetically programmable functionality

Wang

et al. 2020

Sci. Adv.

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Functional coating materials have found broad technological applications in diverse fields. Despite recent advances, few coating materials simultaneously achieve robustness and substrate independence while still retaining the capacity for genetically encodable functionalities. Here, we report Escherichia coli biofilm-inspired protein nanofiber coatings that simultaneously exhibit substrate independence, resistance to organic solvents, and programmable functionalities. The intrinsic surface adherence of CsgA amyloid proteins, along with a benign solution-based fabrication approach, facilitates forming nanofiber coatings on virtually any surface with varied compositions, sizes, shapes, and structures. In addition, the typical amyloid structures endow the nanofiber coatings with outstanding robustness. On the basis of their genetically engineerable functionality, our nanofiber coatings can also seamlessly participate in functionalization processes, including gold enhancement, diverse protein conjugations, and DNA binding, thus enabling a variety of proof-of-concept applications, including electronic devices, enzyme immobilization, and microfluidic bacterial sensors. We envision that our coatings can drive advances in electronics, biocatalysis, particle engineering, and biomedicine.

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Section: Functional Csga Dbd Coating-enabled Microfluidic Bacterial Sensorsmentioning

confidence: 99%

Conformable self-assembling amyloid protein coatings with genetically programmable functionality

Wang

et al. 2020

Sci. Adv.

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“…Furthermore, they are easily synthesized and separated with high sensitivity and specificity through a systematic evolution of ligands by exponential enrichment (SELEX) process which is the key feature of aptamers used for CTCs' isolation . Hence, the aptamer‐functionalized nanostructures were introduced to increase topographic interactions between targeted CTCs and surface coated with specific antibodies, giving rise to recovered CTCs' isolation with satisfactory capture efficiency …”

Section: D Microfluidic Platforms For Ctcs' Detection and Isolationmentioning

confidence: 99%

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

Lin

Luo

et al. 2019

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Cancer remains the leading cause of death worldwide despite the enormous efforts that are made in the development of cancer biology and anticancer therapeutic treatment. Furthermore, recent studies in oncology have focused on the complex cancer metastatic process as metastatic disease contributes to more than 90% of tumor‐related death. In the metastatic process, isolation and analysis of circulating tumor cells (CTCs) play a vital role in diagnosis and prognosis of cancer patients at an early stage. To obtain relevant information on cancer metastasis and progression from CTCs, reliable approaches are required for CTC detection and isolation. Additionally, experimental platforms mimicking the tumor microenvironment in vitro give a better understanding of the metastatic microenvironment and antimetastatic drugs' screening. With the advancement of microfabrication and rapid prototyping, microfluidic techniques are now increasingly being exploited to study cancer metastasis as they allow precise control of fluids in small volume and rapid sample processing at relatively low cost and with high sensitivity. Recent advancements in microfluidic platforms utilized in various methods for CTCs' isolation and tumor models recapitulating the metastatic microenvironment (tumor‐on‐a‐chip) are comprehensively reviewed. Future perspectives on microfluidics for cancer metastasis are proposed.

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“…Various electric fields, magnetic fields, acoustophoresis effects, and hydrodynamic forces have been employed to enhance the efficiency of incubation and partition. Although there are some nice reviews discussing the different types of microfluidic SELEX schemes and applications of aptamer-based microfluidic biosensors (Dembowski and Bowser, 2018;Fraser et al, 2019), there still lacks a review to systematically summarize the microfluidic incubation and partition methodologies and explain their respective pros and cons in different types of SELEX applications. Current review is prepared for this demand, aiming to provide a useful summary for microfluidic experts to introduce the opportunities in aptamer selection, and in the meantime, an understandable guide for SELEX performers to consider microfluidic technologies to upgrade their methodologies.…”

Section: Introductionmentioning

confidence: 99%

The Application of Microfluidic Technologies in Aptamer Selection

Liu¹,

Wang²,

Chan³

et al. 2021

Front. Cell Dev. Biol.

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Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.

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Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Cited by 28 publications

References 172 publications

Conformable self-assembling amyloid protein coatings with genetically programmable functionality

Conformable self-assembling amyloid protein coatings with genetically programmable functionality

Recent Advances in Microfluidic Platforms Applied in Cancer Metastasis: Circulating Tumor Cells' (CTCs) Isolation and Tumor‐On‐A‐Chip

The Application of Microfluidic Technologies in Aptamer Selection

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