Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

Lai, Yu-Ting; Tsai, Chin-Ho; Hsu, Ju‐Chun; Lu, Yen‐Wen

doi:10.3390/mi10120837

Cited by 15 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, implementing these valves in wearable sensors made of paper would require adding electrical actuators and circuitry to the design, which would increase the complexity of the fabrication process. Chemically actuated valves that open upon the addition of organic solvents or surfactants have also been reported. However, organic solvents need to be dispensed manually.…”

Section: Introductionmentioning

confidence: 99%

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

2022

View full text Add to dashboard Cite

Paper sensors with colorimetric signal transduction mechanisms are promising for developing single-use wearable patches that only require a smartphone to quantify signals. However, measuring biomarker fluctuations with colorimetric wearable sensors requires implementing a chrono-sampling method for performing sequential measurements. In this article, we report on a chrono-sampling method that enables the fabrication of wearable devices made entirely of filter paper. It consists of using dried polymers as closed valves that deflect the flow of liquids to different transducers of a multisensor. As time passes by, the polymer dissolves and the valve opens. The sequential opening of the valves results in a succession of measurements that reveals fluctuations in the concentration of the target analyte. This concept was demonstrated with a paper multisensor capable of performing nine consecutive pH measurements. The device was also adapted for developing a urea biosensor that detects pH measurements generated by the hydrolysis of the analyte catalyzed by urease. The proposed analytical platform could monitor the pH of sweat with an accuracy and precision comparable to a laboratory-based method when worn during an exercise routine. The results shown here pave the way for developing colorimetric wearable biosensors that measure variations in the concentration of biomarkers such as glucose, lactate, creatinine, or uric acid over time.

show abstract

Section: Introductionmentioning

confidence: 99%

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

2022

View full text Add to dashboard Cite

show abstract

“…Dissolvable materials have been utilized to delay flow on μPADs, such as pullulan film, surfactant, wax, and sugar . Yager et al reported on the use of dissolvable sucrose on paper to create programmable flow delays to program automated multistep fluidic protocols .…”

Section: Introductionmentioning

confidence: 99%

“…21 Among these methods, colorimetry has been the most commonly used because it is simple, easy to operate, and meets the basic needs for on-site detection without complex equipment. 22 Although many colorimetric ELISA μPADs provide ease of use, rapidity, cost-effectiveness, and portability, only a few have been specifically designed for detecting small targets, 23,24 particularly in relation to monitoring AFB 1 content using competitive ELISA. 25 Our group previously developed the first automated sandwich ELISA for an inkjet-printed μPAD.…”

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Automated Paper-Based Femtogram Sensing Device for Competitive Enzyme-Linked Immunosorbent Assay of Aflatoxin B₁ Using Submicroliter Samples

et al. 2022

View full text Add to dashboard Cite

Microfluidic paper-based analytical devices (μPADs) are promising biosensors that may be used in a variety of bioanalytical applications. A μPAD for automating the competitive enzyme-linked immunosorbent assay (ELISA) of small-sized target detection at the femtogram level using submicroliter samples is reported in this study. The proposed μPAD was integrated with a sucrose valve to automate the sequential delivery of reagents, providing simple control of reagent delivery time and simple operation. The use of a sample solution dropping location at the zones on the device that had been prepared with an antibody-conjugated enzyme before immersion in a running buffer allowed minimization of sample volume to 0.6 μL, while eliminating the possible loss of a target molecule by adsorption on the membrane, thus improving detection sensitivity. Furthermore, the proposed device was successfully applied to the automation of competitive ELISA for the detection of aflatoxin B 1 (AFB 1 ), a potent carcinogen that causes substantial health risks to humans worldwide, with a detection limit of 60 femtograms or 0.1 ng/mL. The method developed in this study provides high sensitivity, small sample volume, on-site and equipment-free measurements, low-cost operation, and user-friendliness. This approach could be used to analyze small-sized molecules in the fields of food safety and quality control, environmental monitoring, and clinical diagnostics.

show abstract

“…Color formed in the detection zone vs. nitrate or nitrite concentration. References [ 46 , 47 , 49 , 59 , 60 ].…”

mentioning

confidence: 99%

A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

Charbaji

Heidari-Bafroui

Anagnostopoulos

et al. 2020

Sensors

View full text Add to dashboard Cite

In this paper, we report a simple and inexpensive paper-based microfluidic device for detecting nitrate in water. This device incorporates two recent developments in paper-based technology suitable for nitrate detection and has an optimized microfluidic design. The first technical advancement employed is an innovative fibrous composite material made up of cotton fibers and zinc microparticles that can be incorporated in paper-based devices and results in better nitrate reduction. The second is a detection zone with an immobilized reagent that allows the passage of a larger sample volume. Different acids were tested—citric and phosphoric acids gave better results than hydrochloric acid since this acid evaporates completely without leaving any residue behind on paper. Different microfluidic designs that utilize various fluid control technologies were investigated and a design with a folding detection zone was chosen and optimized to improve the uniformity of the signal produced. The optimized design allowed the device to achieve a limit of detection and quantification of 0.53 ppm and 1.18 ppm, respectively, for nitrate in water. This accounted for more than a 40% improvement on what has been previously realized for the detection of nitrate in water using paper-based technology.

show abstract

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

Cited by 15 publications

References 35 publications

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

Automated Paper-Based Femtogram Sensing Device for Competitive Enzyme-Linked Immunosorbent Assay of Aflatoxin B₁ Using Submicroliter Samples

A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

Contact Info

Product

Resources

About

Microfluidic Time-Delay Valve Mechanism on Paper-Based Devices for Automated Competitive ELISA

Cited by 15 publications

References 35 publications

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

Dissolvable Polymer Valves for Sweat Chrono-Sampling in Wearable Paper-Based Analytical Devices

Automated Paper-Based Femtogram Sensing Device for Competitive Enzyme-Linked Immunosorbent Assay of Aflatoxin B1 Using Submicroliter Samples

A New Paper-Based Microfluidic Device for Improved Detection of Nitrate in Water

Contact Info

Product

Resources

About

Automated Paper-Based Femtogram Sensing Device for Competitive Enzyme-Linked Immunosorbent Assay of Aflatoxin B₁ Using Submicroliter Samples