Microfluidics-Assisted Diffusion Self-Assembly: Toward the Control of the Shape and Size of Pectin Hydrogel Microparticles

Marquis, Mélanie; Davy, Joëlle; Fang, Aiping; Renard, Denis

doi:10.1021/bm401596m

Cited by 35 publications

(19 citation statements)

References 41 publications

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“…To preserve its bioactivity, TGF-β1 was gently encapsulated inside two EPS-based matrices structured at micro-scale using a capillary microfluidic approach. Over other conventional emulsification techniques used for gelled microparticle generation, microfluidics offers the possibility to produce monodisperse microparticles with tailored sizes and morphologies, without using high energy and temperature conditions, which may be destructive for the bioactive molecule to be encapsulated [29,30]. Another important advantage of microfluidic is the ability to produce microstructures and, in the same time, to encapsulate the totality of the bioactive molecule, in a one-step process [31,32].…”

Section: Introductionmentioning

confidence: 99%

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

et al. 2019

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Articular cartilage is an avascular, non-innervated connective tissue with limited ability to regenerate. Articular degenerative processes arising from trauma, inflammation or due to aging are thus irreversible and may induce the loss of the joint function. To repair cartilaginous defects, tissue engineering approaches are under intense development. Association of cells and signalling proteins, such as growth factors, with biocompatible hydrogel matrix may lead to the regeneration of the healthy tissue. One current strategy to enhance both growth factor bioactivity and bioavailability is based on the delivery of these signalling proteins in microcarriers. In this context, the aim of the present study was to develop microcarriers by encapsulating Transforming Growth Factor-β1 (TGF-β1) into microparticles based on marine exopolysaccharide (EPS), namely GY785 EPS, for further applications in cartilage engineering. Using a capillary microfluidic approach, two microcarriers were prepared. The growth factor was either encapsulated directly within the microparticles based on slightly sulphated derivative or complexed firstly with the highly sulphated derivative before being incorporated within the microparticles. TGF-β1 release, studied under in vitro model conditions, revealed that the majority of the growth factor was retained inside the microparticles. Bioactivity of released TGF-β1 was particularly enhanced in the presence of highly sulphated derivative. It comes out from this study that GY785 EPS based microcarriers may constitute TGF-β1 reservoirs spatially retaining the growth factor for a variety of tissue engineering applications and in particular cartilage regeneration, where the growth factor needs to remain in the target location long enough to induce robust regenerative responses.

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Section: Introductionmentioning

confidence: 99%

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

et al. 2019

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“…9 Several techniques exist to fabricate alginate microparticles such as inkjet printing, 10 spray drying, 11 membrane emulsication 12 or synthesis by a microuidic chip. [13][14][15][16][17][18][19][20][21][22] Overall, synthesis of the alginate microparticles on a custom design microuidic chip appears like the most feasible technique to synthesize monodisperse alginate microparticles. By appropriate design of the microuidic chip, it is possible to prepare a wide range of different alginate microparticles, although it turns out that the size of the microparticles is a limiting factor if blood cell-sized particles are desired.…”

Section: Introductionmentioning

confidence: 99%

“…19 The principle of extraction coupled with both internal and external gelation was also applied to pectin microparticles and the possibility to control not only size but also morphology was demonstrated. 17 The objective of the present work was to achieve a reduction in particle size by the principle of extractive gelation, while exploring the possibility to incorporate functional components (magnetic nanoparticles and liposomes) into the hydrogel matrix. Previously, such functional components have never been incorporated into hydrogel microparticles produced by microuidics.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic fabrication of composite hydrogel microparticles in the size range of blood cells

et al. 2016

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The fabrication of alginate hydrogel microparticles with embedded liposomes and magnetic nanoparticles for radiofrequency controlled release of encapsulated chemical cargo was considered. An extractive gelation process was implemented in a microfluidic device, which enabled the production of uniform composite microparticles of dimensions comparable to those of blood cells (between 5 and 10 μm). The critical parameters that control the extractive gelation process were systematically explored and feasible values that provide microgel particles of a defined size and morphology were identified. First, the initial water-in-oil droplet is formed in a flow-focusing junction whose size is controlled by the flow-rate of the oil phase. Then, the train of droplets is sandwiched between two streams of oil containing calcium ions. In that way, a flux of water molecules from the droplets towards the continuous phase as well as a transport of calcium ions towards the disperse phase are initiated. The final microparticle properties were thus found to be sensitive to three elementary sub-processes: (i) the initial droplet size; (ii) the extraction of water into the oil phase, which was controlled by the volume of the oil phase and its initial moisture content; and (iii) the kinetics of ionic cross-linking of the alginate matrix, which was controlled by the varying calcium concentration. The size and morphology of the final composite microgels were fully characterized

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“…Taking into account all these parameters, microfluidic technology, manipulating multiphase laminar flows to produce homogeneous structures, appeared as a versatile method to generate micrometer-sized droplets with controllable size and functionality. [8][9][10] In order to induce the gelation of the droplets formed in the microfluidic device, two gelation mechanisms are mainly employed, namely external and internal gelation. In the case of external gelation, the cross-linking agent dissolved in the apolar continuous phase diffuses to the droplet phase and initiates its gelation from its surface.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Encapsulation of Pickering Oil Microdroplets into Alginate Microgels for Lipophilic Compound Delivery

Marquis

Alix

Capron

et al. 2016

ACS Biomater. Sci. Eng.

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Alginate microgels are widely used as delivery systems in food, cosmetics, and pharmaceutical industries for encapsulation and sustained release of hydrophilic compounds and cells. However, the encapsulation of lipophilic molecules inside these microgels remains a great challenge because of the complex oil-core matrix required. The present study describes an original two-step approach allowing the easy encapsulation of several oil microdroplets within alginate microgels. In the first step, stable oil microdroplets were formed by preparing an oil-in-water (O/W) Pickering emulsion. To stabilize this emulsion, we used two solid particles, namely the cotton cellulose nanocrystals (CNC) and calcium carbonate (CaCO3). It was observed that the surface of the oil microdroplets formed was totally covered by a CNC layer, whereas CaCO3 particles were adsorbed onto the cellulose layer. This solid CNC shell efficiently stabilized the oil microdroplets, preventing them from undesired coalescence. In the second step, oil microdroplets resulting from the Pickering emulsion were encapsulated within alginate microgels using microfluidics. Precisely, the outermost layer of oil microdroplets composed of CaCO3 particles was used to initiate alginate gelation inside the microfluidic device, following the internal gelation mode. The released Ca2+ ions induced the gel formation through physical cross-linking with alginate molecules. This innovative and easy to carry out two-step approach was successfully developed to fabricate monodisperse alginate microgels of 85 pm in diameter containing around 12 oil microdroplets of 15 mu m in diameter. These new oil-core alginate microgels represent an attractive system for encapsulation of lipophilic compounds such as vitamins, aroma compounds or anticancer drugs that could be applied in various domains including food, cosmetics, and medical applications. Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.

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Microfluidics-Assisted Diffusion Self-Assembly: Toward the Control of the Shape and Size of Pectin Hydrogel Microparticles

Cited by 35 publications

References 41 publications

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

Microcarriers Based on Glycosaminoglycan-Like Marine Exopolysaccharide for TGF-β1 Long-Term Protection

Microfluidic fabrication of composite hydrogel microparticles in the size range of blood cells

Microfluidic Encapsulation of Pickering Oil Microdroplets into Alginate Microgels for Lipophilic Compound Delivery

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