Microfluidics at the interface of bacteria and fresh produce

Ranjbaran, Mohsen; Verma, Mohit S.

doi:10.1016/j.tifs.2022.07.014

Cited by 14 publications

(7 citation statements)

References 79 publications

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“…Additionally, as visual inspection is the only gold standard test currently available for field assessments, we intend to offer fresh produce stakeholders an alternative by providing a quantitative understanding of the potential of fecal contamination in their fields. In the future, it is also possible to conduct these risk assessments in the field by using assays such as loop-mediated isothermal amplification (LAMP) (Davidson et al, 2021;Mohan et al, 2021;Pascual-Garrigos et al, 2021;Ranjbaran and Verma, 2022;Wang et al, 2023Wang et al, , 2022Wang et al, , 2021 as opposed to lab-based qPCR.…”

Section: Bacteroidales Concentration As a Fecal Contamination Biomark...mentioning

confidence: 99%

Bacteroidalesas a Fecal Contamination Indicator in Fresh Produce Industry: A Baseline Measurement

Wang

Ranjbaran

Verma

2023

Preprint

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Foodborne outbreaks caused by fecal contamination of fresh produce represent a serious concern to public health and the economy. As the consumption of fresh produce increases, public health officials and organizations have pushed for improvements in food safety procedures and environmental assessments to reduce the risk of contamination. Visual inspections and the establishment of "buffer zones" between animal feeding operations and producing fields are the current best practices for environmental assessments. However, a generalized distance guideline and visual inspections may not be enough to account for all environmental risk variables. Here, we report a baseline measurement surveying the background Bacteroidales concentration, as a quantitative fecal contamination indicator, in California's Salinas Valley. We collected a total of 1632 samples from two romaine lettuce commercial fields at the time of harvesting through two seasons in a year. The Bacteroidales concentration was very low (0 - 2.00 copies/cm^2). Furthermore, we established a practical methodology for evaluating the risk of fecal contamination in a real-world setting, complementing the current environmental assessment practices. This method can identify site-specific risks and offer fresh produce stakeholders a more comprehensive understanding of their fields. We anticipate this work can encourage the use of Bacteroidales in the fresh produce industry to monitor fecal contamination and prevent future foodborne outbreaks.

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Section: Bacteroidales Concentration As a Fecal Contamination Biomark...mentioning

confidence: 99%

Bacteroidalesas a Fecal Contamination Indicator in Fresh Produce Industry: A Baseline Measurement

Wang

Ranjbaran

Verma

2023

Preprint

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“…Very recently, Yin et al discussed the current state-of-art and future perspectives of detection methods for foodborne viruses. 317 Other recent review articles that include microfluidic devices have been worked out, for instance by Gao et al , who discussed advances in microfluidic devices for foodborne pathogen detection, 318 by Shang et al with focus on advances in nanomaterial-based microfluidic platforms, 319 by Ranjbaran et al on microfluidics at the interface of bacteria and fresh produce, 320 or by Quintela et al on advances and limitations of portable and rapid detection technologies for foodborne pathogens. 321 Mi et al summarized microfluidic biosensor tools for foodborne pathogenic bacteria and Su et al investigated microfluidic nucleic acid tests of foodborne viruses.…”

Section: Gastrointestinal Tract Infectionsmentioning

confidence: 99%

Microfluidic systems for infectious disease diagnostics

Lehnert,

Gijs

2024

Lab Chip

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“…Microfluidics, the science and technology of manipulating tiny volumes of fluid within devices with tailored microscale channels, has emerged as another promising class of biopatterning technologies for efficiently immobilizing bacteria into customized patterns. , Typically, microfluidic devices are made of elastomeric PDMS using multilayer soft lithography. , Common designs for microfluidic cultivating devices include microwells, microdroplets, and microchambers . Microfluidics creates controlled microenvironments for bacterial cells to grow and interact within microcavities of different shapes and sizes .…”

Section: Bacterial Patterning Strategiesmentioning

confidence: 99%

Bacterial Patterning: A Promising Biofabrication Technique

Xiao,

Lv,

Zhu

2024

ACS Appl. Bio Mater.

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Bacterial patterning has emerged as a pivotal biofabrication technique in the biomedical field. In the past 2 decades, a diverse array of bacterial patterning approaches have been developed to enable the precise manipulation of the spatial distribution of bacterial patterns for various applications. Despite the significance of these advancements, there is a deficiency of review articles providing an overview of bacterial patterning technologies. In this mini-review, we systematically summarize the progress of bacterial patterning over the past 2 decades. This review commences with an elucidation of the definition and fundamental principles of bacterial patterning. Subsequently, we introduce the established bacterial patterning strategies, accompanied by discussions about the advantages and limitations of each approach. Furthermore, we showcase the biomedical applications of these strategies, highlighting their efficacy in spatial control of biofilms, biosensing, and biointervention. Finally, this mini-review is concluded with a summary and an outlook on future challenges and opportunities. It is anticipated that this mini-review can serve as a concise guide for those who are interested in this exciting and rapidly evolving research area.

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Microfluidics at the interface of bacteria and fresh produce

Cited by 14 publications

References 79 publications

Bacteroidalesas a Fecal Contamination Indicator in Fresh Produce Industry: A Baseline Measurement

Bacteroidalesas a Fecal Contamination Indicator in Fresh Produce Industry: A Baseline Measurement

Microfluidic systems for infectious disease diagnostics

Bacterial Patterning: A Promising Biofabrication Technique

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