Microfluidics for chiral separation of biomolecules

This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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“…TA B L E 3 Surfactants and modifier used in MEKC and/or MEEKC with example literature [18,31,48,50,68,70,[72][73][74].…”

Section: Intestinal Bacteriamentioning

confidence: 99%

“…Non-ionic Polyoxyethylene dodecyl ether (Brij 35), polyoxyethylene sorbitan monolaurate (Tween-20), cocamide monoethanolamine [18] Polyoxyethylene dodecanol [73] Others (Hydrophobic) Ionic liquids [48] Polymeric surfactants [18] Chiral Review [74] Abbreviation: SDS, sodium dodecyl sulfate.…”

Section: Cationicmentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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“…Enantiomers with identical physical and chemical characteristics tend to behavior-inconsistent biological activities, metabolism, and toxicity in organisms. , This feature renders enantiomeric resolution critical, , and the exploration of innovative techniques or advanced materials is imperative to obtain pure enantiomers. As an emerging class of porous materials, chiral covalent organic frameworks (CCOFs) have been constructed principally by generating the chiral environment in the open channels of covalent organic frameworks (COFs) .…”

Section: Introductionmentioning

confidence: 99%

Chirality-Controlled Mercapto-β-cyclodextrin Covalent Organic Frameworks for Selective Adsorption and Chromatographic Enantioseparation

Cao

et al. 2023

ACS Appl. Mater. Interfaces

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Chiral covalent organic frameworks (CCOFs) benefit from superior stability, abundant chiral environment, and homogeneous pore configuration. In its constructive tactics, only the post-modification method allows for the integration of supramolecular chiral selectors into achiral COFs. Here, the finding utilizes 6-deoxy-6-mercapto-β-cyclodextrin (SH-β-CD) as chiral subunits and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde (DVA) as the platform molecule to synthesize chiral functional monomers through thiol-ene click reactions and directly establish ternary “pendant-type” SH-β-CD COFs. The chiral site density on SH-β-CD COFs was regulated by changing the proportion of chiral monomers to obtain an optimal construction strategy and remarkably improve the ability of chiral separation. SH-β-CD COFs were coated on the inner wall of the capillary in a covalently bound manner. The prepared open tubular capillary was achieved for the separation of six chiral drugs. By combining the outcomes of selective adsorption and chromatographic separation, we observed the higher density of chiral sites in the CCOFs, and poorer results were achieved. From the perspective of spatial conformational distribution, we interpret the variation in the performance of these chirality-controlled CCOFs for selective adsorption and chiral separation.

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“…Microfluidics, also known as lab-on-a chip (LOC), is a device that integrates biomedical analysis experiments, including sample preparation, separation, reaction, detection, etc. [ 1 , 2 , 3 ]. Microfluidic chips can achieve rapid results with a small amount of samples through the precise control of fluids.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to device preparation methods and structural innovations, changing the structure of materials can also play a role in improving the sensing performance, stability and sensitivity of microfluidic devices. Porous materials, with their high specific surface area, good permeability, low relative density, and high specific strength, are widely used in microfluidic chips for applications such as cell separation, cell culture, and real-time biomedical detection [ 2 , 21 , 22 , 23 , 24 ]. Due to its high specific surface area, the porous structure provides a basis for achieving a higher capture rate and provides another idea for improving detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

et al. 2023

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Microfluidics has recently received more and more attention in applications such as biomedical, chemical and medicine. With the development of microelectronics technology as well as material science in recent years, microfluidic devices have made great progress. Porous structures as a discontinuous medium in which the special flow phenomena of fluids lead to their potential and special applications in microfluidics offer a unique way to develop completely new microfluidic chips. In this article, we firstly introduce the fabrication methods for porous structures of different materials. Then, the physical effects of microfluid flow in porous media and their related physical models are discussed. Finally, the state-of-the-art porous microfluidic chips and their applications in biomedicine are summarized, and we present the current problems and future directions in this field.

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Microfluidics for chiral separation of biomolecules

Cited by 8 publications

References 127 publications

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

Chirality-Controlled Mercapto-β-cyclodextrin Covalent Organic Frameworks for Selective Adsorption and Chromatographic Enantioseparation

Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review

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