Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

Jimenez, Mélanie; Bridle, Helen

doi:10.1016/j.mimet.2016.04.008

Cited by 12 publications

(7 citation statements)

References 18 publications

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“…Although conventional PCR and BAX V R System PCR assays allow for Salmonella detection in hours, steps preceding PCR may add days. 13,14 Multiple reports showed that foods could be filtered through membranes in quantities useful for conventional detection procedures (such as plating), [18][19][20] although crossflow microfiltration or ultrafiltration of cells and proteins resulted in fouling of the membrane and limited the volume of sample that could be processed. [21][22][23][24][25][26] We showed that endopeptidase treatment followed by hollow fiber microfiltration was effective in overcoming fouling of the hollow fiber membrane, while avoiding loss of viability of the Salmonella cells.…”

Section: Accelerated Sample Preparationmentioning

confidence: 99%

Protein particulate retention and microorganism recovery for rapid detection of Salmonella

Kreke

Ximenes

et al. 2017

Biotechnology Progress

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The rapid detection of Salmonella in ground meat requires that living microorganisms be brought to levels detectable by PCR, immunoassays, or similar techniques within 8 h. Previously, we employed microfiltration using hollow fiber membranes to rapidly process and concentrate viable bacteria in food extracts through a combination of enzyme treatment and prefiltration in order to prevent blockage or fouling of the hollow fiber membranes. However, scanning electron microscopy and particle size analysis of enzyme hydrolysates showed that enzyme treatment followed by filtration caused submicron particles to form and be trapped within the prefiltration media, which in turn, retained about 80% of the bacteria. Filtering prior to enzyme treatment resulted in formation of a filter cake consisting of protein particles retained on the surface of the filter, while facilitating passage of the much smaller microorganisms through the filter, separating them from particulates. Subsequent enzyme treatment of the filtrate resulted in an extract that was microfiltered in less than an hour, while concentrating viable microorganisms in the extract by 500×. An inoculum of Salmonella enterica cells into turkey burger containing of 1-20 CFU/mL, consisting of spiked cells plus cells already present in the turkey burger sample, was rapidly brought to levels detectable by conventional PCR and BAX PCR assays. The entire procedure from sample processing to detection of Salmonella enterica was achieved in less than 8 h. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:687-695, 2017.

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Section: Accelerated Sample Preparationmentioning

confidence: 99%

Protein particulate retention and microorganism recovery for rapid detection of Salmonella

Kreke

Ximenes

et al. 2017

Biotechnology Progress

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“…18,19 Most of the effort in this field is focused on the control of solid particles with diameters smaller than the size of the channel; typically, by one order of magnitude. [20][21][22][23][24][25][26][27][28][29][30] Although there are similarities between the behavior of rigid particles and bubbles, deformation of the bubbles [31][32][33][34] complicates the understanding of the physical phenomena evidenced in gas/liquid flows. Understanding the active forces affecting bubble motion is necessary in order to explain its behavior.…”

Section: Introductionmentioning

confidence: 99%

Inertial manipulation of bubbles in rectangular microfluidic channels

et al. 2018

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Inertial microfluidics is an active field of research that deals with crossflow positioning of the suspended entities in microflows. Until now, the majority of the studies have focused on the behavior of rigid particles in order to provide guidelines for microfluidic applications such as sorting and filtering. Deformable entities such as bubbles and droplets are considered in fewer studies despite their importance in multiphase microflows. In this paper, we show that the trajectory of bubbles flowing in rectangular and square microchannels can be controlled by tuning the balance of forces acting on them. A T-junction geometry is employed to introduce bubbles into a microchannel and analyze their lateral equilibrium position in a range of Reynolds (1 < Re < 40) and capillary numbers (0.1 < Ca < 1). We find that the Reynolds number (Re), the capillary number (Ca), the diameter of the bubble (D[combining macron]), and the aspect ratio of the channel are the influential parameters in this phenomenon. For instance, at high Re, the flow pushes the bubble towards the wall while large Ca or D[combining macron] moves the bubble towards the center. Moreover, in the shallow channels, having aspect ratios higher than one, the bubble moves towards the narrower sidewalls. One important outcome of this study is that the equilibrium position of bubbles in rectangular channels is different from that of solid particles. The experimental observations are in good agreement with the performed numerical simulations and provide insights into the dynamics of bubbles in laminar flows which can be utilized in the design of flow based multiphase flow reactors.

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“…The source of this fragmentation is most likely high shear forces within the pump and it is recommended to use a progressing cavity pump to mitigate this issue. It can be noted that for smaller inertial focusing channels with similar profile heights (30 and 50 μm high), 100% recoveries have been reported in previous works where syringe pumps have been used293037. However, such pumps cannot be used for recirculating continuously the sample.…”

Section: Resultsmentioning

confidence: 71%

“…For example in environmental monitoring for pathogens such as Cryptosporidium , 1000 L is filtered282930. Other examples include algal dewatering for biofuel production3132, to clean circulating oil in vehicles and heavy rotating machinery33 and bioprocessing/biotechnology34.…”

mentioning

confidence: 99%

Cascading and Parallelising Curvilinear Inertial Focusing Systems for High Volume, Wide Size Distribution, Separation and Concentration of Particles

Miller

Jimenez

Bridle

2016

Sci Rep

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Inertial focusing is a microfluidic based separation and concentration technology that has expanded rapidly in the last few years. Throughput is high compared to other microfluidic approaches although sample volumes have typically remained in the millilitre range. Here we present a strategy for achieving rapid high volume processing with stacked and cascaded inertial focusing systems, allowing for separation and concentration of particles with a large size range, demonstrated here from 30 μm–300 μm. The system is based on curved channels, in a novel toroidal configuration and a stack of 20 devices has been shown to operate at 1 L/min. Recirculation allows for efficient removal of large particles whereas a cascading strategy enables sequential removal of particles down to a final stage where the target particle size can be concentrated. The demonstration of curved stacked channels operating in a cascaded manner allows for high throughput applications, potentially replacing filtration in applications such as environmental monitoring, industrial cleaning processes, biomedical and bioprocessing and many more.

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Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

Cited by 12 publications

References 18 publications

Protein particulate retention and microorganism recovery for rapid detection of Salmonella

Protein particulate retention and microorganism recovery for rapid detection of Salmonella

Inertial manipulation of bubbles in rectangular microfluidic channels

Cascading and Parallelising Curvilinear Inertial Focusing Systems for High Volume, Wide Size Distribution, Separation and Concentration of Particles

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