Microfluidics: IFlowPlate—A Customized 384‐Well Plate for the Culture of Perfusable Vascularized Colon Organoids (Adv. Mater. 46/2020)

Rajasekar, Shravanthi; Lin, Dawn; Abdul, Lyan; Liu, Amy; Sotra, Alexander; Zhang, Feng; Zhang, Boyang

doi:10.1002/adma.202070345

Cited by 12 publications

(14 citation statements)

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“…Moreover, MVNs are often engineered to study interactions with other physiological systems, such as neural or muscular tissues, or used as a platform to host various developing organoids. [32][33][34][35][36] In those multiculture systems, the choice of hydrogel scaffold and composition of culture medium must be carefully designed to support the development and growth of all the cellular com ponents. With our methodology, MVN development in multi culture systems could be substantially improved by employing IF to boost vasculogenesis, significantly broadening the applica tion of selforganized MVNs.…”

Section: Discussionmentioning

confidence: 99%

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase‐2

Zhang

Wan

Pavlou

et al. 2022

Adv Funct Materials

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Self-organized microvascular networks (MVNs) have become key to the development of many microphysiological models. However, the self-organizing nature of this process combined with variations between types or batches of endothelial cells (ECs) often lead to inconsistency or failure to form functional MVNs. Because interstitial flow (IF) has been reported to play a beneficial role in angiogenesis, vasculogenesis, and 3D capillary morphogenesis, the role IF plays during neovessel formation in a customized single-channel microfluidic chip for which IF has been fully characterized is systematically investigated. Compared to static conditions, MVNs formed under IF have higher vessel density and diameters and greater network perfusability. Through a series of inhibitory experiments, this study demonstrates that IF treatment improves vasculogenesis by ECs through upregulation of matrix metalloproteinase-2 (MMP-2). This study then successfully implements a novel strategy involving the interplay between IF and MMP-2 inhibitor to regulate morphological parameters of the self-organized MVNs, with vascular permeability and perfusability well maintained. The revealed mechanism and proposed methodology are further validated with a brain MVN model. The findings and methods have the potential to be widely utilized to boost the development of various organotypic MVNs and can be incorporated into related bioengineering applications where perfusable vasculature is desired.

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Section: Discussionmentioning

confidence: 99%

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase‐2

Zhang

Wan

Pavlou

et al. 2022

Adv Funct Materials

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“…[68][69][70] Moreover, stromal cells will secrete various extracellular matrix (ECM) components to remodel the niche to assist vascular development. [71] Therefore, various stromal cells or mural cells, such as fibroblasts [10,18,19,22,29,63,72] and mesenchymal stem cells (MSCs) [11][12][13][14][15][16][17]23,33] have been introduced into organoid systems along with ECs for the vascularization. Among these cells, fibroblasts are reported to hold great potential to mold the round and elongated shape of blood vessels, inhibit vascular EC apoptosis, and exhibit adjacent fibroblast-cardiomyocyte interactions to improve cardiac development.…”

Section: Stromal Cellsmentioning

confidence: 99%

Vascularization of engineered organoids

Liu

Zhang

Liu

et al. 2023

BMEMat

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Organoids are biomimetic tissue analogs that are generated via the self‐organization of stem cells or adult cells relying on developmental biology principles. They present tissue‐specific structures and functional complexity, bridging the gap between planar cell culture and animal models to accelerate the applications of drug testing, tissue engineering and regenerative medicine. However, the current organoids are still limited in long‐term culture, maturation, complexities and functionalities due largely to the lack of vascularization in the analogs. Herein, we give an overview of the latest development of vascularization in the organoid field. We first provide two typical strategies of self‐assembly and engineering to perform and realize the vascularization in engineering organoids, as well as the crucial factors affecting vasculogenesis. Then, we describe the representative applications using vascularized organoids in biomedical fields. Finally, we discuss the challenges and future perspectives in the development of advanced biomaterials and modified approaches toward building vascularized organoids with higher fidelity and functionality.

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“…[152] Moreover, an increased level of throughput can be achieved by engineering biomimetic models in parallel, for example, recent studies have adapted multi-well plates to develop high-throughput organ-on-chip cultures. [153][154][155] Automating cell and tissue seeding further simplifies the process and improves the level of throughput in these multi-well organ-on-a-chip platforms.…”

Section: Challenges and Future Prospects Of Engineered Microphysiolog...mentioning

confidence: 99%

Next‐generation preclinical models of lung development, physiology and disease

et al. 2022

Self Cite

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The incidence of respiratory diseases such as chronic obstructive pulmonary disease and pulmonary cancer is growing significantly around the world, making pulmonary disease one of the leading causes of mortality. However, the development of effective therapeutics for pulmonary diseases has been hindered by the lack of human-mimetic physiological models that reliably emulate patient responses. Recent advances in technology and cell culture have led to the development of organoids and organ-on-a-chip models that allow us to recapitulate the structure, cellular organization, and organ-level responses of the target tissue in vitro. Here, we review the advances and milestones of lung organoid and lung-on-a-chip models in the past decade and highlight their applications in mimicking pulmonary system development, physiology, disease, and regeneration. In addition, we discuss the ongoing challenges and the future prospects of integrating lung organoids and lung-on-a-chip models to overcome current limitations and to enhance their physiological relevance. These human-centric models are likely to provide important insights into pulmonary physiology and pathophysiology for drug discovery that complement and potentially replace traditional animal models.

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Microfluidics: IFlowPlate—A Customized 384‐Well Plate for the Culture of Perfusable Vascularized Colon Organoids (Adv. Mater. 46/2020)

Cited by 12 publications

References 0 publications

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase‐2

Interstitial Flow Promotes the Formation of Functional Microvascular Networks In Vitro through Upregulation of Matrix Metalloproteinase‐2

Vascularization of engineered organoids

Next‐generation preclinical models of lung development, physiology and disease

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