Microfluidics: The future of microdissection TESE?

Samuel, Reichel; Badamjav, Odgerel; Murphy, Kristin; Patel, Darshan P.; Son, Jiyoung; Gale, Bruce K.; Carrell, Douglas T.; Hotaling, James M.

doi:10.3109/19396368.2016.1159748

Cited by 36 publications

(26 citation statements)

References 70 publications

(79 reference statements)

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“…Additionally, sperm cryopreservation is a critical part of assisted reproductive technology (ART) (Araki et al, 2015; Tomita et al, 2016; Zou et al, 2013), while most of the applications of microfluidics in sperm processing were focused on sorting or isolation of sperm from semen sample; few studies were found on CPA addition and removal (Cho et al, 2003; Huang et al, 2014; Li et al, 2016; Samuel et al, 2016; Sano et al, 2010; Schuster et al, 2003). Fortunately, both the designs for the staggered herringbone microfluidic mixer (Park et al, 2012) and the passive, planar micromixer based on logarithmic spirals (Scherr et al, 2015; Scherr et al, 2012) (Figure 7E), which are initially developed for sperm activation, can be potentially adopted for CPA processing, since where controllable extracellular mixing is supplied.…”

Section: Controllable Addition and Removal Of Cpasmentioning

confidence: 99%

“…Significant progress has been made in recent years for application of microfluidics in ART, e.g., sperm activation, sorting and isolation, oocyte processing, fertilization, and embryo culture (Cho et al, 2003; de Wagenaar et al, 2016; Huang et al, 2014; Li et al, 2016; Ohta et al, 2010; Samuel et al, 2016; Sano et al, 2010; Scherr et al, 2015; Schuster et al, 2003; Suh et al, 2003; Tsai et al, 2010; Zhang et al, 2011b). As an important and indispensable part of fertility preservation, cryopreservation on-chip has also achieved considerable progress, e.g., on-chip CPA-free cryopreservation of small amounts of human spermatozoa was successfully accomplished (Zou et al, 2013).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

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Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano scales, which are presently difficult or impossible with conventional bulk approaches. This review describes applications of microfluidic chips in cell manipulation, cryoprotective agent exposure, programmed freezing/thawing, vitrification, and in situ assessment in cryopreservation, and discusses achievements and challenges, providing perspectives for future development.

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Section: Controllable Addition and Removal Of Cpasmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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“…Important advantages have been shown compared to traditional selection techniques, such as the potential to work with small sperm sample volumes, short processing times, and the ability to manipulate single cells in a non-invasive manner. In addition, the potential to be a versatile tool for selection applications or fundamental studies on sperm has also been shown [24,32]. Furthermore, the yield of the selected sperm by microfluidic technique was estimated at 41%, what is comparable to the recovery rate of currently used conventional methods [33].…”

Section: Microfluidicsmentioning

confidence: 99%

“…The fact that the epigenetically aberrant sperm populations were preferentially found in men with severe sperm abnormalities and that non-imprinted genes are also affected, suggests that there might be a link between phenotype and epigenotype [4]. However, sometimes normal morphology is not necessarily associated with DNA integrity [24]. Moreover, epigenetic markers had significant variation between samples from different men, as well as significant variation within the same semen sample.…”

Section: Microfluidicsmentioning

confidence: 99%

“…Both methods select morphologically normal and motile sperm, and, although these spermatozoa are expected to be epigenetically stable, few studies have been carried out in this direction [22]. Furthermore, there is evidence to support the fact that sperm morphology does not always reflect DNA status [23,24]. It is therefore crucial to find alternative sperm selection methods which allow us to isolate sperm based on novel parameters to better monitor sperm function.…”

Section: Introductionmentioning

confidence: 99%

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Non-Invasive Approaches to Epigenetic-Based Sperm Selection

et al. 2017

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Since sperm size and form do not necessarily provide information on internal sperm structures, novel sperm markers need to be found in order to conduct assisted reproductive therapies (ART) successfully. Currently, the priority of andrologists is not only to select those sperm able to fertilize the oocyte, but also a high quality of sperm that will guarantee a healthy embryo. Evidence of this shows us the importance of studying sperm intensively on genetic and epigenetic levels, because these could probably be the cause of a percentage of infertility diagnosed as idiopathic. Thus, more attention is being paid to posttranslational modifications as the key for better understanding of the fertilization process and its impact on embryo and offspring. Advances in the discovery of new sperm markers should go hand in hand with finding appropriate techniques for selecting the healthiest sperm, guaranteeing its non-invasiveness. To date, most sperm selection techniques can be harmful to sperm due to centrifugation or staining procedures. Some methods, such as microfluidic techniques, sperm nanopurifications, and Raman spectroscopy, have the potential to make selection gentle to sperm, tracking small abnormalities undetected by methods currently used. The fact that live cells could be analyzed without harmful effects creates the expectation of using them routinely in ART. In this review, we focus on the combination of sperm epigenetic status (modifications) as quality markers, with non-invasive sperm selection methods as novel approaches to improve ART outcomes.

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Advances in Microfluidics‐Based Assisted Reproductive Technology: From Sperm Sorter to Reproductive System‐on‐a‐Chip

2018

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The fields of assisted reproductive technology (ART) and in vitro fertilization (IVF) have progressed rapidly, yet still need further improvements. Microfluidic technology can incorporate various ART procedures such as embryo/gamete (sperm/oocyte) analysis, sorting, manipulation, culture, and monitoring. The introduction of paper‐based and droplet‐based microfluidics further improves the commercialization potential of this technology. The progress in 3D printing technology allows for the integration of microfluidics with tissue engineering that may revolutionize current practices in biology and medicine. This review categorizes ART methods according to continuous‐flow microfluidics, paper‐based microfluidics, droplet‐based microfluidics, and organ‐on‐a‐chip. The advances are summarized and potential opportunities in infertility diagnosis, sperm selection, sperm guidance, oocyte selection, insemination, embryo culture, embryo monitoring, and cryopreservation are identified. While some advances of continuous‐flow microfluidics for ART have already been reviewed, other microfluidic techniques are still in their early stages. It is envisioned that advances in droplet‐based microfluidics, especially digital microfluidics, will allow for more progress in human IVF, particularly single embryo transfer. Droplet‐based microfluidics may also lead to fully integrated and high‐throughput platforms for animal IVF. Recent advances in organ‐on‐a‐chip including ovary/uterus/oviduct‐on‐chip platforms hold promise for the integration of the whole human reproductive system‐on‐a‐chip for clinical applications.

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Microfluidics: The future of microdissection TESE?

Cited by 36 publications

References 70 publications

Microfluidics for cryopreservation

Microfluidics for cryopreservation

Non-Invasive Approaches to Epigenetic-Based Sperm Selection

Advances in Microfluidics‐Based Assisted Reproductive Technology: From Sperm Sorter to Reproductive System‐on‐a‐Chip

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