Paleontological inferences, molecular clocks, and biomarker fossils indicate sponges evolved in the Cryogenian, but Precambrian sponge fossils are rare, poorly substantiated, and controversial. Spicule-like microstructures (SLMs) hosted in phosphatized fossils from the Ediacaran Doushantuo Formation (~635-551 Ma) at Weng'an of South China have been interpreted as cylindrical siliceous monaxons, and their hosting fossils as the oldest demosponges in the fossil record. In order to assess their veracity as the oldest spiculate demosponges, we utilize a suite of in situ nanoscale analytical techniques-including scanning electron microscopy, synchrotron Xray fluorescence mapping, X-ray absorption near edge structure (XANES) spectroscopy, focused ion beam electron microscopy, and transmission electron microscopy-to evaluate the ultrastructures and elemental, chemical, and mineralogical compositions of the SLMs. Our data decisively shows that the SLMs are carbonaceous in composition and rectangular in transverse sections, and therefore, are not cylindrical siliceous spicules. Instead, the SLMs may be microbial strands, axial filaments of early hexactinellids, or acicular crystals molded by organic