Microgel dynamics within the 3D porous structure of transparent PEG hydrogels

Bassu, Gavino; Laurati, Marco; Fratini, Emiliano

doi:10.1016/j.colsurfb.2022.112938

Cited by 7 publications

(2 citation statements)

References 62 publications

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“…The large pore structure of hydrogels gives hydrogels good biocompatibility and biosafety in biological applications. 31 As determined by UV spectrophotometry and compared with the concentration standard curve of POM ( Supplement Figure 2 ), the release curve of the GelMA/POM composite hybrid hydrogel was plotted. According to the release curve of the GelMA/POM hydrogel ( Figure 1G ), the release of POM was mainly concentrated in the first 3 days, leading to a rapid increase in POM concentration.…”

Section: Resultsmentioning

confidence: 99%

Combined Molybdenum Gelatine Methacrylate Injectable Nano-Hydrogel Effective Against Diabetic Bone Regeneration

Liao,

Shen,

et al. 2023

IJN

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Introduction Bone defects in diabetes mellitus (DM) remain a major challenge for clinical treatment. Fluctuating glucose levels in DM patients lead to excessive production of reactive oxygen species (ROS), which disrupt bone repair homeostasis. Bone filler materials have been widely used in the clinical treatment of DM-related bone defects, but overall they lack efficacy in improving the bone microenvironment and inducing osteogenesis. We utilized a gelatine methacrylate (GelMA) hydrogel with excellent biological properties in combination with molybdenum (Mo)-based polyoxometalate nanoclusters (POM) to scavenge ROS and promote osteoblast proliferation and osteogenic differentiation through the slow-release effect of POM, providing a feasible strategy for the application of biologically useful bone fillers in bone regeneration. Methods We synthesized an injectable hydrogel by gelatine methacrylate (GelMA) and POM. The antioxidant capacity and biological properties of the synthesized GelMA/POM hydrogel were tested. Results In vitro, studies showed that hydrogels can inhibit excessive reactive oxygen species (ROS) and reduce oxidative stress in cells through the beneficial effects of pH-sensitive POM. Osteogenic differentiation assays showed that GelMA/POM had good osteogenic properties with upregulated expression of osteogenic genes (BMP2, RUNX2, Osterix, ALP). Furthermore, RNA-sequencing revealed that activation of the PI3K/Akt signalling pathway in MC3T3-E1 cells with GelMA/POM may be a potential mechanism to promote osteogenesis. In an in vivo study, radiological and histological analyses showed enhanced bone regeneration in diabetic mice, after the application of GelMA/POM. Conclusion In summary, GelMA/POM hydrogels can enhance bone regeneration by directly scavenging ROS and activating the PI3K/Akt signalling pathway.

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Section: Resultsmentioning

confidence: 99%

Combined Molybdenum Gelatine Methacrylate Injectable Nano-Hydrogel Effective Against Diabetic Bone Regeneration

Liao,

Shen,

et al. 2023

IJN

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“…Image stacks obtained from confocal microscopy were processed and analyzed using MorphoLibJ, an ImageJ plugin that allows the characterization of the 3D structures of pores in the hydrogels [58]. Once the pores were determined in MorphoLibJ, their volume was calculated and used to obtain the pore volume fraction φ = V pores /V tot of the hydrogel, where V pores is the cumulative volume occupied by pores over the total volume of the hydrogel, V tot .…”

Section: Image Analysis To Characterize the Porous Structurementioning

confidence: 99%

Effect of Composition and Freeze-Thaw on the Network Structure, Porosity and Mechanical Properties of Polyvinyl-Alcohol/Chitosan Hydrogels

Soto-Bustamante

Bassu

Fratini

et al. 2023

Gels

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We report the synthesis and characterization of poly (vinyl alcohol) (PVA)/Chitosan (CT) cryogels for applications involving the uptake and entrapment of particulate and bacterial colonies. In particular, we systematically investigated the network and pore structures of the gels as a function of CT content and for different freeze–thaw times, combining Small Angle X-Ray Scattering (SAXS), Scanning Electron Microscopy (SEM), and confocal microscopy. The nanoscale analysis obtained from SAXS shows that while the characteristic correlation length of the network is poorly affected by composition and freeze–thaw time, the characteristic size of heterogeneities associated with PVA crystallites decreases with CT content. SEM investigation evidences a transition to a more homogeneous network structure induced by the incorporation of CT that progressively builds a secondary network around the one formed by PVA. A detailed analysis of confocal microscopy image stacks allows to characterize the 3D porosity of the samples, revealing a significantly asymmetric shape of the pores. While the average volume of single pores increases with increasing CT content, the overall porosity remains almost unchanged as a result of the suppression of smaller pores in the PVA network with the progressive incorporation of the more homogeneous CT network. Increasing the freezing time in the FT cycles also results in a decrease of porosity, which can be associated with a growth in the crosslinking of the network due to PVA crystallization. The linear viscoelastic moduli measured by oscillatory rheology show a qualitatively comparable frequency-dependent response in all cases, with a moderate reduction with increasing CT content. This is attributed to changes in the structure of the strands of the PVA network.

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Synthesis of bioinspired based hydrogel composite from hyaluronic acid/polyacrylic acid and lignin as an adhesive for medical technology

Glingasorn,

Yongsapanan,

Pangon

et al. 2023

emergent mater.

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Microgel dynamics within the 3D porous structure of transparent PEG hydrogels

Cited by 7 publications

References 62 publications

Combined Molybdenum Gelatine Methacrylate Injectable Nano-Hydrogel Effective Against Diabetic Bone Regeneration

Combined Molybdenum Gelatine Methacrylate Injectable Nano-Hydrogel Effective Against Diabetic Bone Regeneration

Effect of Composition and Freeze-Thaw on the Network Structure, Porosity and Mechanical Properties of Polyvinyl-Alcohol/Chitosan Hydrogels

Synthesis of bioinspired based hydrogel composite from hyaluronic acid/polyacrylic acid and lignin as an adhesive for medical technology

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