2024
DOI: 10.1021/acs.biomac.4c00409
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Microgels with Immobilized Glycosyltransferases for Enzymatic Glycan Synthesis

Isabel Katja Sommerfeld,
Philip Palm,
Kai Philip Hussnaetter
et al.

Abstract: Glycans, composed of linked monosaccharides, play crucial roles in biology and find diverse applications. Enhancing their enzymatic synthesis can be achieved by immobilizing enzymes on materials such as microgels. Here, we present microgels with immobilized glycosyltransferases, synthesized through droplet microfluidics, immobilizing enzymes either via encapsulation or postattachment. SpyTag-SpyCatcher interaction was used for enzyme binding, among others. Fluorescamine and permeability assays confirmed enzyme… Show more

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