Microgenomics of Ameloblastoma

Abstract

A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. ABSTRACT Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.

PTCH-1 and MdM2 Expression in Ameloblastoma from A West African Sub-Population: Implication for Chemo-Therapeutics

“…DeVilliers et al [10] had earlier suggested that the over-expression of PTCH could be used for chemotherapeutics and from our findings we support this proposition in Nigerian ameloblastoma cases. Shiori et al [17] recently reported that the proliferation of the ameloblastoma AM-1 cell line was significantly inhibited in the presence of SHH neutralizing antibody and cyclopamine.…”

“…A study by DeVilliers et al [10] that determined the microgenomic profile of ameloblastoma via several molecular methods reported that most members of the Sonic Hedgehog pathway were under-expressed except for PTCH that was over-expressed by two-folds. They additionally validated protein expression in their study by immunohistochemistry.…”

“…Protein Patched homolog 1 (PTCH-1), a tumor suppressor protein, is one of such members. DeVilliers et al [10] identified the overexpression of PTCH-1 in all ameloblastoma cases they studied.…”

Differential expression of canonical and non‐canonical Wnt ligands in ameloblastoma

“…This and the other observed similarity of Wnt‐1 overexpression between UA and KCOT (34) might explain the comparable local invasiveness and recurrence potential in these two neoplasms. Altered Wnt‐10a gene expression in ameloblastoma is putatively mediated by tumor necrosis factor‐α, and this could play an important role in growth and morphological patterning of ameloblastoma (22).…”

Advantages of a combined method of decalcification compared to EDTA

“…It is composed of an association of hydrochloric acid (12%), EDTA (0.07%), sodium tartrate (0.014%), sodium and potassium tartrate (0.8%) and water (bring to 1,000 mL), that we, arbitrarily, named ETDA, to differentiate it from the classic EDTA. Variations in the relative proportions of the components of this decalcifying solution are found in literature (DeVilliers et al, , Kashireddy and Chakravarthy, ).…”