Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis

Zhou, Xi; Huang, Li; Li, Sam Fong Yau

doi:10.1016/s0956-5663(00)00136-6

Cited by 171 publications

(63 citation statements)

References 30 publications

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“…The effect of viscoelasticity certainly exists, as is evident from the increase in motional resistance R m (Figure 5.14). It has been suggested that a correction factor of approximately 2 could be used to correct changes in frequency connected with viscoelasticity (Zhou et al, 2001). However, this is an approximation and should not be viewed as a quantitative, analytical factor.…”

Section: Acoustic Methodsmentioning

confidence: 99%

Detection of Protein–Aptamer Interactions by Means of Electrochemical Indicators and Transverse Shear Mode Method

Hianik

2008

Aptamers in Bioanalysis

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Section: Acoustic Methodsmentioning

confidence: 99%

Detection of Protein–Aptamer Interactions by Means of Electrochemical Indicators and Transverse Shear Mode Method

Hianik

2008

Aptamers in Bioanalysis

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“…Methods for immobilizing proteins on gold film, which have been investigated for years, include the SPA method (15,23 ), the avidin and biotin method (24,25 ), and the self-assembled monolayers method (26,27 ). The SPA method was adopted in the present experiment because it can create more reproducible and stable immobilization layers with less added mass for IgG coating (28 ).…”

Section: Results and Discussion Optimization Of Immobilized Antibody mentioning

confidence: 99%

Rapid and Simultaneous Quantification of 4 Urinary Proteins by Piezoelectric Quartz Crystal Microbalance Immunosensor Array

Luo¹,

Chen²,

Qin

et al. 2006

Clinical Chemistry

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Background: Urinary proteins are predictive and prognostic markers for diabetes nephropathy. Conventional methods for the quantification of urinary proteins, however, are time-consuming, and most require radioactive labeling. We designed a label-free piezoelectric quartz crystal microbalance (QCM) immunosensor array to simultaneously quantify 4 urinary proteins. Methods: We constructed a 2 ؋ 5 model piezoelectric immunosensor array fabricated with disposable quartz crystals for quantification of microalbumin, ␣ 1 -microglobulin, ␤ 2 -microglobulin, and IgG in urine. We made calibration curves after immobilization of antibodies at an optimal concentration and then evaluated the performance characteristics of the immunosensor with a series of tests. In addition, we measured 124 urine samples with both QCM immunosensor array and immunonephelometry to assess the correlation between the 2 methods. Results: With the QCM immunosensor array, we were able to quantify 4 urinary proteins within 15 min. This method had an analytical interval of 0.01-60 mg/L. The intraassay and interassay imprecisions (CVs) were <10%, and the relative recovery rates were 90.3%-109.1%. Nonspecificity of the immunosensor was insignificant (frequency shifts <20 Hz). ROC analyses indicated sensitivities were >95.8% and, specificities were

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“…There is increasing demand for biosensor technology for fast and precise detection of TB with high affinity and specificity. Biosensor technology has the potential to provide a qualitative and quantitative analysis and is free from radioactive or fluorescent tags (Tombelli et al, 2000, Zhou et al, 2001Yao et al, 2008).…”

Section: Role Of Nanobiosensors In Diagnostics Of Tbmentioning

confidence: 99%

Tackling the Problem of Tuberculosis by Nanotechnology

Rai

Ingle

Bansod

et al. 2015

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases

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Microgravimetric DNA sensor based on quartz crystal microbalance: comparison of oligonucleotide immobilization methods and the application in genetic diagnosis

Cited by 171 publications

References 30 publications

Detection of Protein–Aptamer Interactions by Means of Electrochemical Indicators and Transverse Shear Mode Method

Detection of Protein–Aptamer Interactions by Means of Electrochemical Indicators and Transverse Shear Mode Method

Rapid and Simultaneous Quantification of 4 Urinary Proteins by Piezoelectric Quartz Crystal Microbalance Immunosensor Array

Tackling the Problem of Tuberculosis by Nanotechnology

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