1975
DOI: 10.1093/ajcp/64.1.65
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Microhemagglutination Test for the Detection of Nucleoprotein Antibody in Systemic Lupus Erythematosus

Abstract: A simple and practical microhemagglutination test that detects two antibodies, one showing reactivity to the DNA moiety of soluble nucleoprotein (sNP) and the other to the DNA-histone complex of sNP, is described. The method incorporates human erythrocytes formalinized at 30 C., tanned, and coated with sNP at 37 C. Antibody specificity was determined by inhibition experiments performed on sera with added DNA or sNP antigen. With the indirect LE cell technic, evidence that the anti-sNP antibody detected in this… Show more

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