Microimaging FT-IR spectroscopy on pathological breast tissues

Anastassopoulou, J.; Boukaki, E.; Conti, Carla; Ferraris, P.; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Théophanides, T.; Tosi, Giorgio

doi:10.1016/j.vibspec.2009.07.005

Cited by 64 publications

(60 citation statements)

References 21 publications

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“…As it is shown in Figure 1, this band increased in intensity in the spectra of BCC, MM, and NEV, indicating that there was substantial reduction in the amount of hydrogen-bonded C-OH groups of cellular proteins, as well as an accumulation of carbohydrates as a result of glycosylation during the cancer progression. This band was also observed in the cancer regions of breast and colon, and metastatic bone cancers (10,12,22). Figure 2 shows the FT-IR spectra of normal healthy tissue (N), basal call carcinoma (BCC), melanoma (MM) and nevus (NEV) specimens.…”

Section: Resultsmentioning

confidence: 80%

“…As it is known, the hydrogen bond is important in stabilizing the protein helix and any change implies that the physiological environment has changed. We have found that these changes are very important and constitute a basic criterion in order to characterize the disease and its progression (10)(11)(12)(13)(14)(15)(16)(17)(18).…”

Section: Resultsmentioning

confidence: 99%

“…The bands in the spectral region between 3000 cm -1 to 2870 cm -1 ( Figure 1) are assigned to the symmetric and antisymmetric stretching vibrations of methyl (v as CH 3 , v s CH 3 ) and to methylene (v as CH 2 , v s CH 2 ) groups of lipids, proteins, and glycosides (9)(10)(11)(12)15). A considerable increase of the intensity of these bands is observed in the spectra of MM and NEV, and less in BCC spectra, indicating that the environment of the membrane has changed and the surrounding medium has become more lipophilic (15).…”

Section: Resultsmentioning

confidence: 99%

“…Fourier TransformInfrared (FT-IR) spectroscopy provides multidimensional information about the components of a tissue, and can be used as a tool for the non-invasive characterization and identification of the molecular features of various cancer types. The IR spectra consist the "fingerprint" of any material, including tumor tissues and are very sensitive to structural changes of the biological molecules that are formed due to biochemical and metabolic processes that occur during cancer development (9)(10)(11)(12). The mid-IR spectra (4000-400 cm -1 ) of normal and malignant tissues indicated that FT-IR spectroscopy could be useful in the diagnosis of skin cancers (9)(10)(11)(12).…”

mentioning

confidence: 99%

“…The IR spectra consist the "fingerprint" of any material, including tumor tissues and are very sensitive to structural changes of the biological molecules that are formed due to biochemical and metabolic processes that occur during cancer development (9)(10)(11)(12). The mid-IR spectra (4000-400 cm -1 ) of normal and malignant tissues indicated that FT-IR spectroscopy could be useful in the diagnosis of skin cancers (9)(10)(11)(12). FT-IR spectroscopy today is a rapid and easy-to-use method.…”

mentioning

confidence: 99%

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FT-IR Spectroscopy Study in Early Diagnosis of Skin Cancer

Kyriakidou

Anastassopoulou

Tsakiris

et al. 2017

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Section: Resultsmentioning

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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FT-IR Spectroscopy Study in Early Diagnosis of Skin Cancer

Kyriakidou

Anastassopoulou

Tsakiris

et al. 2017

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Analytical Chemistry in the Life Sciences and Medicine

Ham

2016

Kirk-Othmer Encyclopedia of Chemical Technology

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Analytical chemistry, the scientific discipline associated with the study and characterization of both natural and artificial materials, is growing in almost every area. Qualitative and quantitative methods are used in a complementary fashion for the separation, identification, and quantification of analytes that make up the sample under investigation.Analytical methods can be broken down into two areas: traditional benchtop (also called wet chemistry) and instrumental analysis—the driving force behind almost all recent advances—that employs sophisticated computerized apparatus with specialized software for measurement. Recent improvements in instrumental analyses focus on applications to a broader scope of disciplines, including bioanalysis, forensics, military, environmental analysis, industrial analysis, materials analysis, and medical and clinical analysis. Biological applications (bioanalytical chemistry) such as cancer and biomarker studies are of special interest and therefore are the focus of this article.

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Characterizing biological macromolecules with attenuated total reflectance–Fourier transform infrared spectroscopy provides hands‐on spectroscopy experiences for undergraduates

Helburn

Nolan

2022

Biochem Molecular Bio Educ

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Infrared (IR) spectra of biologically derived materials display distinct absorption bands correlating to individual macromolecules: protein, polysaccharide, lipid, and nucleic acids. A series of experiments aimed at teaching qualitative bioorganic spectroscopy using attenuated total reflectance (ATR) Fourier transform infrared (IR) with biological polymers as samples is proposed. Labs targeting 1st and 4th year undergraduate students at St. Francis College are being developed. During 2014 ➔ 2017, an integrated biology/chemistry exercise featuring elementary spectroscopy as an addition to an existing lab on light microscopy was administered to three sections of a 1st year general biology course. Students were taught the concept of a spectral fingerprint and to identify carbohydrate and protein based materials by looking for key vibrational bands. The success of that effort as determined by the results of an assessment quiz became the motivation for developing an advanced 4th year exercise involving four macromolecules. In a trial lab (Spring 2019) students gathered reference spectra from materials homogeneous in a single biopolymer followed by spectra of whole tissues which they were expected to fully characterize. Assessment data suggest that 1st year students benefited most from the experience. A detailed discussion of reference and sample spectra (as obtained by students) and relevant bond vibrations along with suggestions for instructors are presented.

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Microimaging FT-IR spectroscopy on pathological breast tissues

Cited by 64 publications

References 21 publications

FT-IR Spectroscopy Study in Early Diagnosis of Skin Cancer

FT-IR Spectroscopy Study in Early Diagnosis of Skin Cancer

Analytical Chemistry in the Life Sciences and Medicine

Characterizing biological macromolecules with attenuated total reflectance–Fourier transform infrared spectroscopy provides hands‐on spectroscopy experiences for undergraduates

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