Micromanipulation of amyloplasts with optical tweezers in &lt;i&gt;Arabidopsis&lt;/i&gt; stems

Abe, Yoshinori; Meguriya, Keisuke; Matsuzaki, Takahisa; Sugiyama, Teruki; Yoshikawa, Hiroshi; Morita, Miyo Terao; Toyota, Masatsugu

doi:10.5511/plantbiotechnology.20.1201a

Cited by 8 publications

(2 citation statements)

References 58 publications

(70 reference statements)

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“…It is expected that the position of amyloplasts determines the LZY4 accumulation site on the PM independent of the direction of gravity. To test this possibility, amyloplasts were manipulated by an optical tweezer during observation by confocal laser scanning microscopy ( 46 ). After trapping some amyloplasts, the amyloplasts were manipulated into close proximity of the PM (figs.…”

Section: Amyloplast Position Determines Lzy4 Polarity At the Pmmentioning

confidence: 99%

Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane

Nishimura

Mori

Shikata

et al. 2023

Science

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Organisms have evolved under gravitational force and many sense the direction of gravity via statoliths in specialized cells. In flowering plants, starch-accumulating plastids, known as amyloplasts, act as statoliths to facilitate downstream gravitropism. The gravity sensing mechanism has long been considered a mechanosensing process by which amyloplasts transmit forces to intracellular structures, but the molecular mechanism underlying this has not been elucidated. We show here that LAZY1-LIKE (LZY) family proteins involved in statocyte gravity signaling associate with amyloplasts and its proximal plasma membrane. This results in polar localization according to the direction of gravity. We propose a gravity sensing mechanism by which LZY translocation to the plasma membrane signals the direction of gravity by transmitting information on the position of amyloplasts.

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Section: Amyloplast Position Determines Lzy4 Polarity At the Pmmentioning

confidence: 99%

Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane

Nishimura

Mori

Shikata

et al. 2023

Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Then, it is expected that the position of amyloplasts determines the LZY4 accumulation site on the PM independent of the direction of gravity. To test this possibility, amyloplasts were manipulated by an optical tweezer during observation by confocal laser scanning microscopy (41). After trapping some amyloplasts, the amyloplasts were manipulated to put them in close proximity to the PM (figs.…”

Section: Main Textmentioning

confidence: 99%

Cell polarity linked to gravity sensing is generated by protein translocation from statoliths to the plasma membrane

Nishimura

Mori

Shikata

et al. 2023

Preprint

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Organisms that have evolved under the gravitational force sense the direction of gravity via statoliths in specialized cells. In the gravitropism of flowering plants, the starch-accumulating plastids, amyloplasts, in gravity sensing cells act as statoliths. The gravity sensing mechanism has long been considered a mechanosensing process by which amyloplasts transmit forces to intracellular structures, but the molecular support has not been reported. This study revealed that LAZY1-LIKE family proteins involved in gravity signaling in statocytes are localized to the amyloplast periphery and its proximal plasma membrane, resulting in polar localization according to the direction of gravity. We propose a gravity sensing mechanism by which LZY transmits the positional information of amyloplasts, i.e., the direction of gravity, by translocating to the plasma membrane.

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Nickel–cobalt layered double hydroxide as a saturable absorber for continuous wave mode-locked laser

Du,

Qi,

et al. 2024

Appl. Phys. B

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Micromanipulation of amyloplasts with optical tweezers in <i>Arabidopsis</i> stems

Cited by 8 publications

References 58 publications

Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane

Cell polarity linked to gravity sensing is generated by LZY translocation from statoliths to the plasma membrane

Cell polarity linked to gravity sensing is generated by protein translocation from statoliths to the plasma membrane

Nickel–cobalt layered double hydroxide as a saturable absorber for continuous wave mode-locked laser

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