Micromanipulation of Human Oocytes and Embryos: Applications of Intracytoplasmic Sperm Injection and Assisted Hatching in Infertility Treatment

Aston, Kenneth I.; Weimer, Klaus E.

doi:10.1007/978-1-4419-1436-1_40

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Assisted Hatching1

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2018

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“…There are some factors that impair hatching, including abnormality in ZP structure, increase in ZP thickness, and zona hardening. Advanced maternal age, ovarian stimulation protocol, sub-optimal in vitro culture and cryopreservation may induce zona hardening (Aston and Weimer 2010). Assisted hatching (AH), by creating an opening in the ZP, helps the embryo to escape from the ZP.…”

Section: Assisted Hatchingmentioning

confidence: 99%

Cleavage-stage embryo micromanipulation in the clinical setting

Halvaei

Ghazali

Nottola

et al. 2018

Systems Biology in Reproductive Medicine

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ART: assisted reproductive technology; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; PGD: preimplantation genetic diagnosis; PZD: partial zona dissection; ZP: zona pellucida; SUZI: subzonal insemination; PVS: perivitelline space; AH: assisted hatching; LAH: laserassisted hatching; ZT: zona thinning; UV: ultraviolet; IR: infrared; PCR: polymerase chain reaction; FISH: fluorescent in situ hybridization; NGS: next generation sequencing; QC: quality control; QA: quality assurance.

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Section: Assisted Hatchingmentioning

confidence: 99%