Micromethod for determination of orthophosphate in presence of adenosine triphosphate after deproteinization with trichloroacetate buffer

Abstract

To reduce these effects, the sample size must be kept small, heat leakage between the samples must be kept to a minimum and heat transfer to the samples must be rapid. These last two requirements are conflicting as a high diffusivity block, which is necessary for rapid heat transfer to the samples, will allow heat transfer to take place fairly easily between the samples. However, when considering heat transfer between the samples, account must be taken of the temperature profile between them. This is not linear but has the form of a IF so that heat cannot readily pass from one sample to the other.If, however, a reaction occurs in which the temperature drops or rises so as to distort the W, then heat will be transferred. This effect can be reduced by the use of small samples, using electronic amplification to increase the size of the recorded peaks. However, when studying mixtures, small sample size may be detrimental to good mixing and a compromise sample, which is large enough to give good mixing, but small enough to reduce heat leakage effects must be used.

Determination of nucleoside triphosphatase activities from measurement of true inorganic phosphate in the presence of labile phosphate compounds

“…In some protocols, the phosphomolybdate complex is quickly extracted in alcohol before acidic conditions cause appreciable hydrolysis of labile OPCs in the analyte [29,30]. While these methods can improve the selectivity of the phosphomolybdate assay for P i , they often decrease simplicity, while increasing time and cost.…”