Micromonospora profundi sp. nov., isolated from deep marine sediment

Abstract

A novel actinobacterial strain, designated DS3010T, was isolated from a Black Sea marine sediment and characterized using a polyphasic approach. The strain was shown to have chemotaxonomic, morphological and phylogenetic properties consistent with classification as representing a member of the genus Micromonospora. Comparative 16S rRNA gene sequence studies showed that the strain was most closely related to the type strains of Micromonospora saelicesensis (99.5 %), Micromonospora chokoriensis (99.4 %) and Micromonospora violae (99.3 %). Similarly, a corresponding analysis based on partial gyrB gene sequences showed that it formed a distinct phyletic branch in a subclade that included the type strains of Micromonosporazamorensis, 'Micromonospora zeae', 'Micromonospora jinlongensis', M. saelicesensis and Micromonospora lupini. DS3010T was distinguished from its closest phylogenetic neighbours by low levels of DNA-DNA relatedness and by a combination of chemotaxonomic and phenotypic properties. On the basis of these data, it is proposed that the isolate should be assigned to the genus Micromonospora as Micromonospora profundi sp. nov. with isolate DS3010T (=DSM 45981T=KCTC 29243T) as the type strain.

Defining the Species Micromonospora saelicesensis and Micromonospora noduli Under the Framework of Genomics

“…The topology clearly showed the close relationship between the two groups as visualized by the branch lengths which were almost inexistent. Group II ( M. noduli ) also showed a close relationship with the type strains of Micromonospora profundi isolated from a deep marine sediment (Veyisoglu et al, 2016 ) and Micromonospora ureilytica isolated from Pisum sativum (Carro et al, 2016 ), also recovered in this group. Reported DDH values between M. saelicesensis and M. ureilytica and M. profundi (Veyisoglu et al, 2016 ) were 28.4 and 56.9%, respectively.…”

“…Group II ( M. noduli ) also showed a close relationship with the type strains of Micromonospora profundi isolated from a deep marine sediment (Veyisoglu et al, 2016 ) and Micromonospora ureilytica isolated from Pisum sativum (Carro et al, 2016 ), also recovered in this group. Reported DDH values between M. saelicesensis and M. ureilytica and M. profundi (Veyisoglu et al, 2016 ) were 28.4 and 56.9%, respectively. A DDH value of 50.9% was found between M. noduli GUI 43 T and M. ureilytica GUI23 T (Carro et al, 2016 ).…”

Micromonospora pelagivivens sp. nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan

“…The family Micromonosporaceae, including the genus Micromonospora, is a widely distributed group from marine environments [3][4][5]. This family was first proposed by Krasil'nikov [6].…”

Draft Genome Sequence of Micromonospora sp. Rc5 Isolated from desert Egyptian Soil

“…Micromonospora is the type genus of the family Micromonosporaceae, which are Gram positive, filamentous, aerobic bacteria which have diverse habitats such as soil, water, marine sediment and mangrove (1)(2)(3) . This family produces many of the well-known antibiotics such as the aminoglycosides gentamicin and amikacin (4) .…”