Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin

Sun, Xiujun; Qiu, Shiwen; Luo, Xianxian; Jin, Pinjiao; Zhao, Junwei; Wu, Xianyao; Yang, Jize; Wang, Xiangjing; Song, Jia; Xiang, Wensheng

doi:10.1007/s10482-021-01550-6

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…The G+C content of the genomic DNA varies from 67–76 mol%. Actinoplanes species are known to produce bioactive compounds [4, 5], such as the antibiotic teicoplanin and the alpha-glucosidase inhibitor acarbose [6]. Actinoplanes species are aerobic, Gram-stain positive, non-acid-fast actinomycetes, capable of forming branched substrate mycelia on various media, although aerial mycelia are rare [7].…”

Section: Introductionmentioning

confidence: 99%

Actinoplanes sandaracinus sp. nov., a novel ligninase- producing and cellulose-degrading actinobacterium isolated from soil

Wang,

Xu,

Zhou

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A novel ligninase-producing and cellulose-degrading actinobacterium, designated strain NEAU-A12T, was isolated from a soil sample collected from Aohan banner, Chifeng City, Inner Mongolia Autonomous Region, PR China. A polyphasic taxonomic study was used to establish the status of strain NEAU-A12T. 16S rRNA gene sequence analysis revealed that strain NEAU-A12T belonged to the genus Actinoplanes and showed the highest similarity (98.3 %) to Actinoplanes palleronii DSM 43940T, while showing less than 98.3 % similarity to other members of the genus Actinoplanes . The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and glycosylphosphatidylinositol. The diagnostic sugars in cell hydrolysates were determined to be arabinose, glucose and xylose. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The major fatty acids were C15 : 0, C16 : 0, C16 : 1 ω7c and C17 : 0. Meanwhile, genomic analysis revealed a genome size of 10 192 524 bp and a DNA G+C content of 70.6 mol%, and indicated that strain NEAU-A12T had the potential to degrade lignin and cellulose, as well as produce bioactive compounds. In addition, the average nucleotide identity values between strain NEAU-A12T and its reference strains A. palleronii DSM 43940T, Actinoplanes regularis DSM 43151T, Actinoplanes philippinensis DSM 43019T, Actinoplanes xinjiangensis DSM 45184T and Actinoplanes italicus DSM 43146T were 80.3, 80.3, 84.1, 84.3 and 84.0 %, respectively. The levels of digital DNA–DNA hybridization between them were found to be 23.6 % (21.3–26.1 %), 23.8 % (21.5–26.3 %), 28.3 % (25.9–30.8 %), 28.6 % (26.0–30.9 %) and 28.4 % (26.2–31.1 %), respectively. Based on phenotypic, chemotaxonomic and genotypic data, strain NEAU-A12T is considered to represent a novel species of the genus Actinoplanes , for which the name Actinoplanes sandaracinus sp. nov. is proposed, with NEAU-A12T (=CCTCC AA 2020039T=DSM 112043T) as the type strain.

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Section: Introductionmentioning

confidence: 99%

Actinoplanes sandaracinus sp. nov., a novel ligninase- producing and cellulose-degrading actinobacterium isolated from soil

Wang,

Xu,

Zhou

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Validation List no. 216. Valid publication of new names and new combinations effectively published outside the IJSEM

Oren,

Göker

2024

International Journal of Systematic and Evolutionary Microbiology

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Molecular Identification and Determination of Antimicrobial Activity of Micromonospora and Nonomuraea Isolates

Koçak

2022

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Aktinobakteriler farklı ekosistemlerde yaşayabilen ve sekonder metabolit açısından zengin kaynak oluşturan bakteri grubunun en büyük üyesini oluşturmaktadır. Nadir aktinobakteriler ise aktif metabolitler açısından önemli hedeflerden biri haline gelmiştir. Nadir aktinobakterilerden olan Micromonospora ve Nonomuraea son dönemde en çok ilgi çeken cinslerdendir. Sunulan çalışmada endemik bir türden Micromonospora ve Nonomuraea cins üyelerinin izolasyonu hedeflenmiştir. İzolatların moleküler tanımlamalarında 16S rRNA gen bölgesi analizleri kullanılmıştır. Analizler sonucunda 10 izolatın Micromonospora üyesi, 2 izolatın Nonomuraea üyesi olduğu belirlenmiştir. Nonomuraea sp. HCI 01 ve HCI 02 suşları Micromonospora sp. HCI 04, HCI 23, HCI 39, HCI 44 ve HCI 49 suşlarının olası yeni birer tür olma potansiyeline sahip olduğu tespit edilmiştir. Antimikrobiyal aktivite çalışmalarında Micromonospora sp. HCI 45, HCI 47 ve HCI 49 suşları E. coli’ye karşı, HCI 20 ve HSF 02 S. aureus’a karşı ve HCI 04 ve HSF 02 ise S. cerevisiae’a karşı etkinlik gösterdiği belirlenmiştir. Nonomuraea sp. ise HCI 02 S. cerevisiae’a karşı antimikrobiyal aktiviteye sahip olduğu görülmüştür. Elde edilen sonuçlarla, olası yeni türlerin olduğu ve antibiyotik olma potansiyeline sahip biyolojik aktif moleküllerin varlığı gösterilmiştir.

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Micromonospora rubida sp. nov., a novel actinobacterium isolated from soil of Harbin

Cited by 3 publications

References 68 publications

Actinoplanes sandaracinus sp. nov., a novel ligninase- producing and cellulose-degrading actinobacterium isolated from soil

Actinoplanes sandaracinus sp. nov., a novel ligninase- producing and cellulose-degrading actinobacterium isolated from soil

Validation List no. 216. Valid publication of new names and new combinations effectively published outside the IJSEM

Molecular Identification and Determination of Antimicrobial Activity of Micromonospora and Nonomuraea Isolates

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