Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala

Abstract

A novel endophytic actinomycete, strain 274745 T , was isolated from a root of Sonneratia apetala collected in a mangrove forest in Sanya, Hainan province, China. The 16S rRNA gene sequence of strain 274745T showed the greatest similarity to Micromonospora pattaloongensis TJ2-2 T (98.3 %). Phylogenetic analysis based on the gyrB gene also supported the close relationship of these two strains. The predominant menaquinone was MK-10(H 8 ) and the major fatty acids were iso-C 15 : 0 , C 17 : 0 and anteiso-C 15 : 0 . The characteristic whole-cell sugars were xylose and mannose. The cell wall contained meso-diaminopimelic acid and glycine. The polar lipid profile mainly comprised phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The DNA G+C content was 71.6 mol%. Furthermore, a combination of DNA-DNA relatedness and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 274745 T represents a novel species of the genus Micromonospora, for which the name Micromonospora sonneratiae sp. nov. is proposed. The type strain is 274745 T (5CCTCC AA 2012003 T 5DSM 45704 T ).

Industrial Enzymes and Metabolites from Actinobacteria in Food and Medicine Industry

“…coastal sediments in Wales [14], marine sediments in Mexico [15] and peat swamp forests in Thailand [16]. The genus have been found forming intimate associations with plants on their leaves [17], roots [18,19] and various plant rhizospheres [20,21] including rhizospheres of biofuel crops growing on marginal lands [22], from nitrogen-fixing root nodules of the actinorhizal plant Casuarina equisetifolia [23,24] and Coriaria myrtifolia [25], and also from root nodules of the leguminous plants Lupinus angustifolius [26] and Pisum sativum [27]. Furthermore, Micomonospora inhabits nitrogen-fixing nodules in a systematic way [28].…”

Micromonospora zeae sp. nov., a novel endophytic actinomycete isolated from corn root (Zea mays L.)

“…15 Growth at different temperatures (4, 16,18,22,28,37, 45 1C) was determined on ISP 3 agar after incubation for 2 weeks. pH range (pH 4, 5, 6,7,8,9,10) and NaCl tolerance (0, 1, 2, 3, 4, 5, 6, 7 and 8%, w/v) for growth were determined on GY medium 16 in shake flasks (250 r.p.m.) at 28 1C for 7 days.…”

“…4 In the last few years, six novel endophytic species of genus Micromonospora were isolated from different plants, including M. coriariae, 5 M. lupini, 6 M. saelicesensis, 6 M. pisi, 7 M. avicenniae 8 and M. sonneratiae. 9 Two novel anthraquinones, lupinacidins A and B with significant antitumor activity were isolated from M. lupini. 10 As part of a program to discover actinomycetes with novel antibiotic production properties, strain NEAU-gq9 T was isolated from corn root (Zea mays L.).…”

Micromonospora nickelidurans sp. nov., isolated from soil from a nickel-mining site

“…Members of this genus typically exhibit single, non-motile spores on the substrate mycelium and lack aerial mycelium (Ren et al , 2013). At the time of writing, a total of 55 species in the genus Micromonospora have validly published names, including the recently described Micromonospora polyrhachis (Xiang et al , 2014), Micromonospora wenchangensis (Ren et al , 2013), Micromonospora equina (Everest & Meyers, 2013), Micromonospora schwarzwaldensis (Gurovic et al , 2013), Micromonospora sonneratiae (Li et al , 2013), Micromonospora sediminicola (Supong et al , 2013), Micromonospora kangleipakensis (Nimaichand et al , 2013). Members of the genus Micromonospora are widely distributed in different environments, but so far no species has been discovered in mine soils contaminated with heavy metals.…”