Micromorphological, anatomical and molecular study of Hedera species (Araliaceae) in Iran

Abstract

Hedera, with 12 extant species, is a genus of evergreen climbers native to Europe, north Africa, and south Asia. In this study, the micromorphological, anatomical structure and molecular evidences of 11 populations from two species of Hedera (H. helix and H. pastuchovii) have been considered to evaluate the relationships in Hedera. In total, seven quantitative and qualitative characters of pollen were selected and measured. Based on this study, the anticlinal wall and surface sculpturing of seed support for separation of two species of Hedera. Micromorphology of epidermis illustrated two types of epidermal cells: puzzle-shaped and polygonal cells. Using nuclear (nrDNA ITS) marker, we reconstructed phylogenetic relationships within two species of Hedera. This data set was analyzed by phylogenetic methods including Bayesian inference, maximum likelihood, and maximum parsimony. In phylogenetic analyses, all members of two species formed a well-supported clade (PP = 1; ML/BS = 100/100) and divided into two major clades (A and B). Neighbor Net diagram demonstrated separation of the studied populations. The results showed that these taxa differ in taxonomically important micromorphological, anatomical and molecular characteristics and these data provide reliable evidence for separation of these two species.

Localisation of active substances in Hedera helix leaves and Linaria vulgaris shoots used in pharmaceutical and cosmetic industry

“…As Hart and Henwood [2006] and Wen [2011] demonstrated, the indumentum in the araliaceans often comprises simple or multicellular stellate trichomes. As suggested by Amini et al [2019] and Lestari and Elya [2019], the presence of secretory ducts, calcium oxalate crystals, and non-glandular trichomes has great diagnostic importance in Hedera spp. and the family Araliaceae.…”

“…It supports the data by Săvulescu and Luchian [2009], who showed that these cells had thin walls, prominent nuclei, and dense cytoplasm. As shown by Amini et al [2019], H. helix petioles have 9 to 12 secretory ducts, whereas their number in H. pastuchovii petioles is lower, i.e. from 6 to 8.…”

Evaluation of tropical–temperate transitions: An example of climatic characterization in the Asian Palmate group of Araliaceae

“…Clarke, and Heteropanax Seem. ; Jebb, 1998 ; Shang et al, 2000 ; Jamir and Pandey, 2003 ; Heriyanto and Sawitri, 2007 ; Prabhu et al, 2010 ; Tagane et al, 2017 ; Jiménez‐Montoya and Idárraga‐Piedrahíta, 2018 ; Ong, 2018 ; Amini et al, 2020 ). Because most of these localities lacked longitude and latitude coordinates, coordinates were determined using GeoLocate Web Application (Rios and Bart, 2010 ).…”

Macromorphological, anatomical and molecular studies of some Araliaceae taxa in Egypt

“…and Sche era pueckleri are the modern names of Sciadophyllum pulchrum leaves. Amini et al(2019) studied the micromorphology of Hedera species in Iran. Lestari and Elya (2019) made macroscopic studies of Polyscias guilfoylei leaves.…”