Micromorphological and Anatomical Evaluation of In Vitro and Field Transferred Plants of Coccinia indica

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

“…The variation in sizes of the tissue structural components could be due to the differences in size and age of the in vitro regenerated and maternal A. africana plant parts compared. Similar observations were made by Shekhawat and Manokari (2018) who noted that in vitro regenerated plants possessed smaller leaves, thinner stems, and roots. However, during acclimatization, these plant parts grew thicker and larger ( Shekhawat and Manokari, 2018 ).…”

“…Similar observations were made by Shekhawat and Manokari (2018) who noted that in vitro regenerated plants possessed smaller leaves, thinner stems, and roots. However, during acclimatization, these plant parts grew thicker and larger ( Shekhawat and Manokari, 2018 ). The presence of well-developed and fully differentiated plant tissues may be related to their functionality ( Kim et al, 2021 ).…”

“…Chlorophyll fluorescence assessment in PSII has been used in a number of studies to determine the photosynthetic rate in various plants, including Triticum turgidum L. ( Moustaka et al, 2018 ), P. africana ( Komakech et al, 2020 ), and Pseudotsuga menziesii ( Perks et al, 2001 ). The lower Fv/Fm values in the first 2 weeks of measurement for in vitro regenerated plants could be attributed to poorly differentiated and underdeveloped photosynthetic tissues of in vitro plantlets ( Shekhawat and Manokari, 2018 ). When in vitro regenerated plants are exposed long enough to the external environment, the leaf mesophyll differentiates and tissues become more developed, thus fully adapting the plants for photosynthesis ( Abd El-Zaher, 2008 ; Batagin-Piotto et al, 2012 ; Shekhawat and Manokari, 2018 ).…”

“…The lower Fv/Fm values in the first 2 weeks of measurement for in vitro regenerated plants could be attributed to poorly differentiated and underdeveloped photosynthetic tissues of in vitro plantlets ( Shekhawat and Manokari, 2018 ). When in vitro regenerated plants are exposed long enough to the external environment, the leaf mesophyll differentiates and tissues become more developed, thus fully adapting the plants for photosynthesis ( Abd El-Zaher, 2008 ; Batagin-Piotto et al, 2012 ; Shekhawat and Manokari, 2018 ). The Fv/Fm values of the in vitro regenerated A. africana plants from the third until the last week of measurements ranged from 0.821 ± 0.0047 to 0.824 ± 0.0037 and those of the maternal plants ranged from 0.823 ± 0.0042 to 0.823 ± 0.0037, indicating that they had similar photosynthetic rates.…”

Attenuation of morpho-anatomical disorders of micropropagated plantlets of Crinum malabaricum Lekhak and Yadav using seismo-mechanical stimulation

“…The aforesaid in vitro-induced foliar morpho-anatomical and functional deformities must be corrected before unwrapping the culture vessels and introduction of plantlets to the field environment, to enhance the survival of tissue culture raised plantlets and successful development of commercial propagation protocol for any species of interest (Chandra et al 2010;Bairu and Kane 2011;Bag et al 2019). The morpho-anatomical and physiological assessment of the in vitro plantlets could help to understand the abnormalities and conquer the problems of tissue culture raised plantlets for successful acclimatization and in the prediction of the survival rate under field conditions (Moyo et al 2015;Shekhawat and Manokari 2018;Pavao et al 2019).…”