2017
DOI: 10.1038/s41467-017-01764-1
|View full text |Cite
|
Sign up to set email alerts
|

Microneedle-array patches loaded with dual mineralized protein/peptide particles for type 2 diabetes therapy

Abstract: The delivery of therapeutic peptides for diabetes therapy is compromised by short half-lives of drugs with the consequent need for multiple daily injections that reduce patient compliance and increase treatment cost. In this study, we demonstrate a smart exendin-4 (Ex4) delivery device based on microneedle (MN)-array patches integrated with dual mineralized particles separately containing Ex4 and glucose oxidase (GOx). The dual mineralized particle-based system can specifically release Ex4 while immobilizing G… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

1
121
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
6

Relationship

0
6

Authors

Journals

citations
Cited by 173 publications
(122 citation statements)
references
References 64 publications
1
121
0
Order By: Relevance
“…To this end, SF plays a primary role in increasing the stiffness of our hybrid MN‐array patch, which is also a function of monomer concentration and crosslinking density of the semi‐IPN hydrogel (Table S2, Supporting Information). The mechanical strength of the MN‐array patch fabricated with a monomer concentration of 0.6 m and 20% crosslinking density was 0.738 ± 0.112 N per needle ( Figure A), which provided a 12.3‐fold margin of safety over the force required for insertion into the skin (0.06 N per needle) without breaking (Figure B), remarkably stronger than the majority of the hydrogel‐forming MNs reported to date . The hybrid MN‐array patch was able to effectively penetrate mouse and porcine skin, as evidenced by microchannels formed on the skin, based on trypan blue staining (Figure C) and SEM imaging (Figure D).…”
Section: Resultsmentioning
confidence: 94%
See 2 more Smart Citations
“…To this end, SF plays a primary role in increasing the stiffness of our hybrid MN‐array patch, which is also a function of monomer concentration and crosslinking density of the semi‐IPN hydrogel (Table S2, Supporting Information). The mechanical strength of the MN‐array patch fabricated with a monomer concentration of 0.6 m and 20% crosslinking density was 0.738 ± 0.112 N per needle ( Figure A), which provided a 12.3‐fold margin of safety over the force required for insertion into the skin (0.06 N per needle) without breaking (Figure B), remarkably stronger than the majority of the hydrogel‐forming MNs reported to date . The hybrid MN‐array patch was able to effectively penetrate mouse and porcine skin, as evidenced by microchannels formed on the skin, based on trypan blue staining (Figure C) and SEM imaging (Figure D).…”
Section: Resultsmentioning
confidence: 94%
“…In summary, we developed an “enzyme‐free” polymeric MN‐array patch composed of a boronate‐gel/SF semi‐IPN hydrogel, which can provide on‐demand and automated insulin delivery with an aqueous stability of at least 2 months and a sustained performance durability. Achieving both (at once) fast and long‐lasting glucose‐responsiveness has been a challenge with currently available chemically controlled closed‐loop systems . Note that all reported GO x ‐nanoparticle‐based insulin‐loaded MNs suffer from a short duration of action (typically on the order of several hours even for small animals), due to their burst‐responsive and transient (irreversible particle disassociation) natures .…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Effective delivery of proteins with enzymatic or regulatory activity has been developed using MN technology, including insulin, desmopressin, erythropoietin, lysozyme, glucagon, glucagon-like peptide-1 (GLP-1), parathyroid hormone (PTH), growth hormone and etanercept [6,42,63]. The selections of materials and formulation to preserve the protein drug stability remain to be a challenge, particularly in large-scale storage schemes and in production chains for clinical use.…”
Section: Types Of Delivered Proteinmentioning
confidence: 99%
“…Using diabetic C57BL/6 db/db mice as a model, they demonstrated the crosslinked alginate patch loaded with 300 μg Ex4 could control the BG of mice for 5 to 6 days. Furthermore, by integrating the mineralized particles, the failure force of the needles was significantly increased to facilitate skin penetration [63]. …”
Section: Representative Types Of Polymeric Mnmentioning
confidence: 99%