Microneedle arrays integrated with living organisms for smart biomedical applications

Cai, Bo; Gong, Yusheng; Wang, Zheng; Wang, Lin; Chen, Wei

doi:10.7150/thno.66478

Cited by 23 publications

(17 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results provide us with a better understanding of the characteristics of the immune pool in normal Chinese people and a method which provides a foundation to study age‐related diseases and monitor treatments, thus improving future treatments. This study shows the importance of conducting larger studies of repertoire analysis from different ages, populations, and health status, as this can be used as disease markers thus aiding in early diagnosis 52–55 and personalized treatment 56–60 …”

Section: Discussionmentioning

confidence: 95%

Immune repertoire analysis of normal Chinese donors at different ages

et al. 2022

View full text Add to dashboard Cite

Objectives: This study investigated the characteristics of the immune repertoire in normal Chinese individuals of different ages. Materials and Methods:In this study, all seven receptor chains from both B and T cells in peripheral blood of 16 normal Chinese individuals from two age groups were analyzed using high-throughput sequencing and dimer-avoided multiplex PCR amplification. Normal in this study is defined as no chronic, infectious or autoimmune disease within 6 months prior to blood draw. Results:We found that compared with the younger group, the clonal expression of T-cell receptor repertoire increased in the older group, while diversity decreased. In addition, we found that the T-cell receptor repertoire was more significantly affected by age than the B-cell receptor repertoire, including significant differences in the use of the unique TCR-alpha and TCR-beta V-J gene combinations, in the two groups of normal participants. We further analyzed the degree of complementarity determining region 3 sequence sharing between the two groups, and found shared TCR-alpha, TCR-gamma, immunoglobulin-kappa and immunoglobulin-lambda chain complementarity determining region 3 sequences in all subjects. Conclusion:Taken together, our study gives us a better understanding of the immune repertoire of different normal Chinese people, and these results can be applied to the treatment of age-related diseases. Immune repertoire analysis also allows us to observe participant's wellness, aiding in early-stage diagnosis. | INTRODUCTIONThe Adaptome is a collection of all the structural and functional diversity of T cell receptors (TCRs) and B cell receptors (BCRs) of an individual at any given time, which accurately and comprehensively reflects the dynamic changes of the immune system. 1 Within the Cailing Song and Wenjing Pan have contributed equally to this study and share first authorship.

show abstract

Section: Discussionmentioning

confidence: 95%

Immune repertoire analysis of normal Chinese donors at different ages

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Microneedles (MNs), which are basically needles in the scale of micrometers at most, are robustly growing [ 1 ] pioneers of the minimally invasive injection procedures, enabling more advanced delivery/collecting systems [ 2 , 3 ]. In comparison with conventional needle technologies, MNs are verified to be more advantageous by decreasing the administration pain [ 4 ] and curtailment of anxiety and needle phobia [ 5 ], along with being more cost-efficient for drug delivery [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Machine Learning-Enabled Prediction of 3D-Printed Microneedle Features

2022

View full text Add to dashboard Cite

Microneedles (MNs) introduced a novel injection alternative to conventional needles, offering a decreased administration pain and phobia along with more efficient transdermal and intradermal drug delivery/sample collecting. 3D printing methods have emerged in the field of MNs for their time- and cost-efficient manufacturing. Tuning 3D printing parameters with artificial intelligence (AI), including machine learning (ML) and deep learning (DL), is an emerging multidisciplinary field for optimization of manufacturing biomedical devices. Herein, we presented an AI framework to assess and predict 3D-printed MN features. Biodegradable MNs were fabricated using fused deposition modeling (FDM) 3D printing technology followed by chemical etching to enhance their geometrical precision. DL was used for quality control and anomaly detection in the fabricated MNAs. Ten different MN designs and various etching exposure doses were used create a data library to train ML models for extraction of similarity metrics in order to predict new fabrication outcomes when the mentioned parameters were adjusted. The integration of AI-enabled prediction with 3D printed MNs will facilitate the development of new healthcare systems and advancement of MNs’ biomedical applications.

show abstract

“…Hence parasite products are not considered for clinical application. To address the challenge of the immunotherapy of parasite-related products in T1DM, based on our previous success in microneedlebased strategies [39][40][41][42][43], herein we develop an asymmetric microneedle patch for the continuous and safe release Fig. 1 Immunological mechanism of STAMP application on the skin during the type 1 diabetes treatment.…”

Section: Introductionmentioning

confidence: 99%

Immunotherapy for type 1 diabetes mellitus by adjuvant-free Schistosoma japonicum-egg tip-loaded asymmetric microneedle patch (STAMP)

Huang

Chen

et al. 2022

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

Background Type 1 diabetes mellitus (T1DM) is an autoimmune disease mediated by autoreactive T cells and dominated by Th1 response polarization. Insulin replacement therapy faces great challenges to this autoimmune disease, requiring highly frequent daily administration. Intriguingly, the progression of T1DM has proven to be prevented or attenuated by helminth infection or worm antigens for a relatively long term. However, the inevitable problems of low safety and poor compliance arise from infection with live worms or direct injection of antigens. Microneedles would be a promising candidate for local delivery of intact antigens, thus providing an opportunity for the clinical immunotherapy of parasitic products. Methods We developed a Schistosoma japonicum-egg tip-loaded asymmetric microneedle patch (STAMP) system, which serves as a new strategy to combat TIDM. In order to improve retention time and reduce contamination risk, a specific imperfection was introduced on the STAMP (asymmetric structure), which allows the tip to quickly separate from the base layer, improving reaction time and patient’s comfort. After loading Schistosoma japonicum-egg as the immune regulator, the effects of STAMP on blood glucose control and pancreatic pathological progression improvement were evaluated in vivo. Meanwhile, the immunoregulatory mechanism and biosafety of STAMP were confirmed by histopathology, qRT-PCR, ELISA and Flow cytometric analysis. Results Here, the newly developed STAMP was able to significantly reduce blood glucose and attenuate the pancreatic injury in T1DM mice independent of the adjuvants. The isolated Schistosoma japonicum-eggs micron slowly degraded in the skin and continuously released egg antigen for at least 2 weeks, ensuring localization and safety of antigen stimulation. This phenomenon should be attributed to the shift of Th2 immune response to reduce Th1 polarization. Conclusion Our results exhibited that STAMP could significantly regulate the blood glucose level and attenuate pancreatic pathological injury in T1DM mice by balancing the Th1/Th2 immune responses, which is independent of adjuvants. This technology opens a new window for the application of parasite products in clinical immunotherapy.

show abstract

Microneedle arrays integrated with living organisms for smart biomedical applications

Cited by 23 publications

References 143 publications

Immune repertoire analysis of normal Chinese donors at different ages

Immune repertoire analysis of normal Chinese donors at different ages

Machine Learning-Enabled Prediction of 3D-Printed Microneedle Features

Immunotherapy for type 1 diabetes mellitus by adjuvant-free Schistosoma japonicum-egg tip-loaded asymmetric microneedle patch (STAMP)

Contact Info

Product

Resources

About