Microneedle based electrochemical (Bio)Sensing: Towards decentralized and continuous health status monitoring

García-Guzmán, Juan José; Pérez‐Ràfols, Clara; Cuartero, María; Crespo, Gastón A.

doi:10.1016/j.trac.2020.116148

Cited by 85 publications

(92 citation statements)

References 162 publications

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“…2d. The elasticity of the MN is 0.78 N.mm -2 , which demonstrates a value that is suitable for initial insertion into a material like skin [18].…”

Section: Mechanical Behavior Of Gold-coated Mnsmentioning

confidence: 88%

Ex vivo performance evaluation of a transdermal gold injectable microneedle for tyrosinase sensing

Darvishi

Girault

2021

Preprint

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Here, the semi-invasive direct electrochemical detection of melanoma biomarkers in non-treated skin has been envisaged. The enzyme tyrosinase (TYR) was to be addressed with a microneedle electrochemical sensor. The microneedles were fabricated by polydimethylsiloxane (PDMS) casting with stable polymers. The as-prepared microneedles (MNs) were then coated by gold sputtering. The gold MNs were finally covered by alginate/catechol to provide a liquid electrolyte layer that contained electroactive species, such as catechol, whose redox state can be linked to the concentration of TYR in the skin. The sensor showed high sensitivity of 7.52 µA·mg–1·mL TYR in dummy skin. A relative standard deviation (RSD) of 8.54 % (n=5) demonstrated that the Catechol@alginate: gold MN electrode had excellent reproducibility for continuous glucose detection. Also, five parallel Catechol@alginate: gold MN electrodes were fabricated using the same experimental setup and showed an RSD of 3.95 % (n=5). These results suggest that the electrode fabrication process was reproducible, and Catechol@alginate: gold MN biosensors demonstrated high stability for the repetitive detection of TYR. Furthermore, the sensor has high selectivity in the presence of interfering components towards TYR screening.

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“…2d. The elasticity of the MN is 0.78 N.mm -2 , which demonstrates a value that is suitable for initial insertion into a material like skin [18].…”

Section: Mechanical Behavior Of Gold-coated Mnsmentioning

confidence: 88%

Ex vivo performance evaluation of a transdermal gold injectable microneedle for tyrosinase sensing

Darvishi

Girault

2021

Preprint

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“…Particularly in regards to the validation issue, any mismatch between on-body measurements and the gold standard technique may arise from the technique used for sweat collection compromising the accuracy of the results. 5 , 85 Currently, the use of adsorbent pads seems to be the most common strategy for such a purpose. 45 , 46 , 78 …”

Section: Wearable Sweat Lactate Sensorsmentioning

confidence: 99%

Can Wearable Sweat Lactate Sensors Contribute to Sports Physiology?

Hoovels¹,

Xuan

Cuartero

et al. 2021

ACS Sens.

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The rise of wearable sensors to measure lactate content in human sweat during sports activities has attracted the attention of physiologists given the potential of these “analytical tools” to provide real-time information. Beyond the assessment of the sensing technology per se , which, in fact, has not rigorously been validated yet in controlled conditions, there are many open questions about the true usefulness of such wearable sensors in real scenarios. On the one hand, the evidence for the origin of sweat lactate (e.g., via the sweat gland, derivation from blood, or other alternative mechanisms), its high concentration (1–25 mM or even higher) compared to levels in the blood, and the possible correlation between different biofluids (particularly blood) is rather contradictory and generates vivid debate in the field. On the other hand, it is important to point out that accurate detection of sweat lactate is highly dependent on the procedure used to collect and/or reach the fluid, and this can likely explain the large discrepancies reported in the literature. In brief, this paper provides our vision of the current state of the field and a thoughtful evaluation of the possible reasons for present controversies, together with an analysis of the impact of wearable sweat lactate sensors in the physiological context. Finally, although there is not yet overwhelming scientific evidence to provide an unequivocal answer to whether wearable sweat lactate sensors can contribute to sports physiology, we still understand the importance to bring this challenging question up-front to create awareness and guidance in the development, validation, and implementation of wearable sensors.

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“…The applications of electrochemical MNs for the detection of most of these biotargets have recently been reviewed [37]. Moreover, the advantages and challenges of using electrochemical MNs for in vivo ISF extraction have been thoroughly discussed elsewhere [38]. Nevertheless, there is still a lack of a comprehensive review paper that systematically investigates the design consideration, fabrication, and sensing applications of MNs for ISF extraction and monitoring.…”

Section: Introductionmentioning

confidence: 99%

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

et al. 2021

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Dermal interstitial fluid (ISF) is a novel source of biomarkers that can be considered as an alternative to blood sampling for disease diagnosis and treatment. Nevertheless, in vivo extraction and analysis of ISF are challenging. On the other hand, microneedle (MN) technology can address most of the challenges associated with dermal ISF extraction and is well suited for long-term, continuous ISF monitoring as well as in situ detection. In this review, we first briefly summarise the different dermal ISF collection methods and compare them with MN methods. Next, we elaborate on the design considerations and biocompatibility of MNs. Subsequently, the fabrication technologies of various MNs used for dermal ISF extraction, including solid MNs, hollow MNs, porous MNs, and hydrogel MNs, are thoroughly explained. In addition, different sensing mechanisms of ISF detection are discussed in detail. Subsequently, we identify the challenges and propose the possible solutions associated with ISF extraction. A detailed investigation is provided for the transport and sampling mechanism of ISF in vivo. Also, the current in vitro skin model integrated with the MN arrays is discussed. Finally, future directions to develop a point-of-care (POC) device to sample ISF are proposed.

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Microneedle based electrochemical (Bio)Sensing: Towards decentralized and continuous health status monitoring

Cited by 85 publications

References 162 publications

Ex vivo performance evaluation of a transdermal gold injectable microneedle for tyrosinase sensing

Ex vivo performance evaluation of a transdermal gold injectable microneedle for tyrosinase sensing

Can Wearable Sweat Lactate Sensors Contribute to Sports Physiology?

Microneedle Arrays for Sampling and Sensing Skin Interstitial Fluid

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