Microneutralisation systems for use with different strains of peste des petits ruminants virus and rinderpest virus

Abstract

Comparative studies were made to determine the most suitable microtitration system for assaying strains of peste des petits ruminants virus (PPRV) and rinderpest virus (RV). Infectivity titres did not differ significantly when assayed in either calf kidney, sheep kidney or Vero cells. However, cytopathic effects were much easier to detect in the latter making them the cell of choice. Addition of small amounts of virus to preformed cell monolayers in microplates with the subsequent addition of maintenance medium give higher infectivity titres than when cell suspension was added to virus, although the latter is more convenient for routine use. The titres of PPRV and neutralising antibodies assayed in tubes and microplates were not significantly different. Simultaneous screening of sera at a 1 in 20 dilution against both PPRV and RV gave a higher incidence of positives against homologous as opposed to heterologous virus.

Evaluation of morbillivirus exposure in cetaceans from the northern Gulf of Mexico 2010-2014

“…Antibody titers against cetacean morbilliviruses were measured using the microtiter VN test (Rossiter et al 1985), as described by Saliki & Lehenbauer (2001). Titers were expressed as the reciprocal of the highest dilution of serum that completely neutralized cytopathic effects in duplicate wells.…”

Diagnosis and control of peste des petits ruminants: a comprehensive review

“…Therefore, specific diagnosis of PPRV infection can be achieved by cDNA hybridization [41,105,127,149], mobility of N protein [40,149], neutralization test [28,116,149] and MAb-based ELISA [81,82,135,136], RT-PCR [7,53] and Real time RT-PCR [12,13,18]. A battery of serological tests and molecular assays are available to detect and identify PPRV antigen/nucleic acid and antibodies.…”

“…Virus isolation cannot always be done as routine diagnostic assays because they are time-consuming and cumbersome and require cell culture facilities, and they are not as sensitive as RT-PCR [27]. VNT was performed for confirmation and differentiation of RP and PPR viruses [55,78], either in tubes or in microtitre plates (micro-VNT) for the detection of PPRV antibodies in serum samples [116,149]. Differential neutralization test is one of the important means to distinguish RP and PPR viruses [116,149] and a micro-VNT has been employed to measure the infectivity titre of RPV and PPRV in calf kidney, sheep kidney and Vero cells [116].…”

“…VNT was performed for confirmation and differentiation of RP and PPR viruses [55,78], either in tubes or in microtitre plates (micro-VNT) for the detection of PPRV antibodies in serum samples [116,149]. Differential neutralization test is one of the important means to distinguish RP and PPR viruses [116,149] and a micro-VNT has been employed to measure the infectivity titre of RPV and PPRV in calf kidney, sheep kidney and Vero cells [116]. Although PPRV clinically and antigenically related to RPV, PPRV is distinguishable serologically not only from RPV but also from MV and CDV [58].…”

Assessment of the duration of maternal antibodies specific to the homologous <i>peste des petits</i> ruminant vaccine “Nigeria 75/1" in <i>Djallonké</i> lambs

“…In each flock, 56 ewes, 3 years old, and negative for rinderpest and PPR specific antibodies were randomly selected. The serology status of selected animals was determined by performing the comparative viral neutralising test 12 .…”