2001
DOI: 10.1246/cl.2001.138
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Micronized Gel Electrophoresis: Construction and Its Versatile Applications

Abstract: We have constructed a one-inch-sized apparatus for gel electrophoresis (µGE). This apparatus was shown to be of high performance and to have potentials to apply to versatile purposes mainly due to its speed and economy. The temperature gradient gel electrophoresis as well as the conventional gel electrophoresis of multi-wells was successfully performed with both DNAs and proteins. Secondary gel electrophoreses in the two alternative modes, band-cut and zone-cut, were shown to be operable.

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“…Temperature gradient gel electrophoresis (TGGE) (Thatcher and Hodson, 1981) enables us to measure the DNA sequence-inherent melting temperature , for which the device can be miniaturized to a size of square inches without losing accuracy (Biyani et al, 2001;Tominaga, 2006). In this study, a µTGGE apparatus, μ-TG (TAITEC, Japan) was used with a specifically fabricated gel cassette.…”
Section: µTgge (Temperature Gradient Gel Electrophoresis) Analysismentioning
confidence: 99%
“…Temperature gradient gel electrophoresis (TGGE) (Thatcher and Hodson, 1981) enables us to measure the DNA sequence-inherent melting temperature , for which the device can be miniaturized to a size of square inches without losing accuracy (Biyani et al, 2001;Tominaga, 2006). In this study, a µTGGE apparatus, μ-TG (TAITEC, Japan) was used with a specifically fabricated gel cassette.…”
Section: µTgge (Temperature Gradient Gel Electrophoresis) Analysismentioning
confidence: 99%