Micronuclei assessment in buccal cells of people environmentally exposed to arsenic in northern Chile

Martínez, Valeria P.; Creus, A.; Venegas, W.; Arroyo, Amparo; Beck, Jens; Gebel, Thomas; Surrallés, Jordi; Marcos, Ricard

doi:10.1016/j.toxlet.2004.10.007

Cited by 66 publications

(25 citation statements)

References 32 publications

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“…The induction of MN after exposure to As 2 O 3 in our study was found to be dose dependent which is in accordance with the earlier published studies [50,51]. Both CAs and MN are genotoxicity markers [52][53][54][55][56][57]. Although their presence does not lead to adverse effects, their high-level indicates that cell has been exposed to the carcinogens.…”

Section: Sharma and Gillsupporting

confidence: 92%

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

Sharma

Gill

2018

Asian J Pharm Clin Res

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Objective: The present study investigated the antigenotoxic effect of Zingiber officinale (Ginger) on arsenic-induced genotoxic effects in chromosome structure of Swiss albino mice.Methods: Albino mice were divided into four groups: Group I was kept as control, Group II was administrated an oral dose of arsenic trioxide 5 mg/kg bw for 15 days, Group III was given an oral dose of arsenic trioxide 5 mg/kg bw followed by 20 mg/kg bw of ginger orally, and Group IV was administered with 20 mg/kg bw of ginger orally for 15 days and kept as positive control. The autopsies were done from all the groups on 15-day posttreatment, and bone marrow was removed for chromosomal study and for calculating the frequency of micronuclei (MN). Results:It is concluded from this study that long-term arsenic exposure causes chromosomal anomalies, for example, breaks, gaps, rings, stretching, and stickiness which leads to the fragmentation of chromosomes and increases the frequency of MN. Conclusion:The administration of ginger showed modulatory effects against arsenic-induced genotoxicity.

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Section: Sharma and Gillsupporting

confidence: 92%

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

Sharma

Gill

2018

Asian J Pharm Clin Res

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“…Finally, increased levels of DNA damage were measured in buccal epithelial cells from individuals chronically exposed to As via drinking water in Inner Mongolia, China, using the DNA Ladder and Tunel assay . Although most studies have detected increased levels of DNA damage, SCE, MN or CAs in As-exposed populations, there was no increase in micronucleated buccal cells associated with As ingestion in a group of individuals from Nevada [Warner et al, 1994], and higher but not significantly higher frequencies of MN were detected in the buccal cells of a population from northern Chile that was environmentally exposed to As [Martinez et al, 2005]. …”

Section: Discussionmentioning

confidence: 99%

Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

Vuyyuri¹,

Ishaq²,

Kuppala³

et al. 2006

Environ and Mol Mutagen

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Arsenic (As) is a known human carcinogen; however, very little is known about the health consequences of occupational exposure to As. In the present study, we assessed the genotoxic damage in the blood cells and in the buccal cells of south Indian glass factory workers who are occupationally exposed to As. The As content in the whole blood of 200 workers and 165 controls was evaluated with inductively coupled plasma mass spectrometry. Blood leukocytes from the subjects were monitored for the level of DNA damage using the Comet assay (mean comet tail length); buccal cells were used to determine the frequency of micronuclei (MN). The mean As concentration was significantly higher in the workers (56.76 microg/L) than in the controls (11.74 microg/L) (P < 0.001). The workers also had increased frequencies of MN in the buccal cells and increased levels of DNA damage in leukocytes compared to the controls (P < 0.001). There were significant correlations between the genotoxicity endpoints that were evaluated and blood As concentration, smoking, age, and the duration of working in the factory. Also, a significant correlation was observed between the frequency of MN and comet tail-length for the worker samples. Our findings indicate that chronic occupational exposure to As is genotoxic and that the Comet assay and micronucleus test are useful assays for evaluating genotoxicity in humans occupationally exposed to As.

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“…Micronuclei appear in the cytoplasm as small condensed chromatin bodies fragmented from acentric chromosomes or whole chromosomes [35]. Comet assay, single cell gel electrophoresis, is a powerful tool to detect genotoxicity, DNA damage/repair and biomonitoring for clastogenic or bio-materials in eukaryotes [27].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Genotoxicity of Gold Nanorods in Mouse Bone Marrow Compared with Cyclophosphamide

Gamal‐Eldeen¹,

Abo-Zeid²,

El‐Daly³

et al. 2016

Nano BioMed ENG

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Gold nanorods (GNRs) are now under extensive investigation for biomedical applications. The in vivo genotoxic profile GNRs are not elucidated yet, therefore we investigated it in comparison with one of the most effective chemotherapeutic agents, cyclophosphamide (CP), in a mice model. PEGylatedGNRs (50 nm) were injected to Balb/C mice triple times, while CP-treated mice were treated once and the bone marrow cells (BMCs) were collected after 21 days. Chromosome aberrations, mitotic index, sister chromatid exchanges (SCEs), replicative index, micronucleus (MN) and DNA damage using comet assay were investigated. GNRs induced chromosome aberrations including-and excluding-gaps significantly at p < 0.001 and p < 0.01, respectively, however CP resulted in a higher significant increase in both types with p < 0.001. The percentage of SCEs/cell was not affected by GNRs treatment, while it was extremely significant with CP. Both mitotic activity and proliferative index were reduced dramatically with both of GNRs and CP. The recorded MN were lower in GNRsthan CP-treated mice. The percentage DNA damage, tail length and tail moment were higher in CP than GNRs. In conclusion, CP induced extreme genotoxicity more than GNRs. Both of GNRs and CP induced DNA damage. The study indicated the advantage of lower GNRs genotoxicity than that of CP. After 21 days, one injection of CP led to extreme persistent genotoxic effect more than that of multiple injections of GNRs.

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Micronuclei assessment in buccal cells of people environmentally exposed to arsenic in northern Chile

Cited by 66 publications

References 32 publications

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

Restorative Effects of Zingiber Officinale on Arsenic-Induced Genotoxic Effects in Chromosomal Structure of Mice

Evaluation of micronucleus frequencies and DNA damage in glass workers exposed to arsenic

In Vivo Genotoxicity of Gold Nanorods in Mouse Bone Marrow Compared with Cyclophosphamide

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