Micronuclei frequency as an early diagnostic tool for detection of Oral Cancer: A comparative study

Gupta, Akanksha; Bhovi, Thimmarasa V; Jaju, Prashant; Gupta, Ankit; Gupta, Manika; Shrivastava, Kriti

doi:10.5958/2395-499x.2016.00014.9

Cited by 3 publications

(5 citation statements)

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“…This is similar to the study conducted by Gupta et al (2011). [14] The study conducted by Casartelli et al concluded that the gradual increase in micronuclei frequency from normal mucosal to pre-cancerous lesion to carcinoma proposed a link of this biomarker with neoplastic progression. [15] There was stepwise increase found in the percentage of micronucleated cells and micronuclei from control to pre-cancer patients and from pre-cancer to cancer patients in the studies by Saran et al and Grover et al [16][17] In the study conducted by Kumar et al, the cells from OSCC lesions were obtained by mincing the biopsy tissue and preparing single cell suspensions from it.…”

Section: Frequency Of Micronuclei In Oral Potentially Malignant Disorsupporting

confidence: 86%

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

2018

IJDA

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Background: Early recognition is crucial for successful treatment and good prognosis of oral cancer. A simple, noninvasive, and reasonably effective technique for initial evaluation of a suspicious oral lesion are exfoliative cytology. Micronuclei being structures presenting after genomic damage can be used as a screening tool in early detection of cancer. Method: A total of 60 subjects, 30 with potentially malignant disorders (PMD) (oral lichen planus and oral submucous fibrosis), 15 with oral squamous cell carcinoma (OSCC), and 15 with apparently normal mucosa, were included in the study. Cytological smears were collected from these groups and micronuclei were examined using Papanicolaou stain. One-way ANOVA was used to compare the mean micronuclei distribution among different study groups. Results: An increase in the frequency of micronuclei from normal to PMD to cancerous lesions was observed. Analysis has shown significant differences between various study groups (P < 0.05), with highest score observed in OSCC group, intermediary in PMD, and least in controls. Conclusion: The oral mucosal micronuclei can be used as a marker of epithelial carcinogenic progression.

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Section: Frequency Of Micronuclei In Oral Potentially Malignant Disorsupporting

confidence: 86%

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

2018

IJDA

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“…Thus it has been concluded that a gradual increase in the micronuclei count from normal mucosa to precancerous to carcinoma thereby suggesting a link of this biomarker with neoplastic progression. The initiation and progression of cancer has now been largely attributed to the role played by inflammatory cells such as cytokines and growth factors like chemokines [ 7 , 9 , 10 ]. The lateral border of the tongue was found to be the most common site of tumor occurrence in both smokers and nonsmokers.…”

Section: Discussionmentioning

confidence: 99%

“…They can be detected in exfoliated cells micronucleus assay which involves microscopic analysis of epithelial smears to determine the prevalence of micronucleation, an indicator of structural or numerical chromosome aberrations. The assay is reliable and technically easy to perform, noninvasive and sensitive with limited cost [5][6][7]. The rationale of the study is to emphasize the severity of CMI due to sharp teeth which also will enable health professionals and the public health workers to plan methods of the appropriate approach in handling this condition and the necessity of early precautionary treatment in individuals identified with a risk factor.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic analysis of genetic damage in young individuals exposed to Chronic Mechanical Irritation in the lateral border of the tongue due to sharp teeth – A novel study.

Yoithapprabhunath¹,

Ganapathy

Renugadevi

et al. 2020

Medicine and Pharmacy Reports

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Background. Chronic inﬂammation is estimated to contribute to approximately 25% of human cancers. Inﬂammation can be induced by chronic mechanical irritation which can lead to oral pre-cancer and oral cancer. Though many studies have been conducted to identify the genetic damage in oral cancer or dysplastic stages, no study has been completed so far on early detection of genetic damage in healthy individuals presenting with sharp teeth in contact with the lateral border of the tongue. Objective. The study is aimed at analyzing the genetic damage (micronuclei) in healthy individuals with sharp teeth in contact with the lateral border of the tongue causing chronic mechanical irritation. Methods. The study group comprised of 75 clinically healthy individuals with sharp teeth in contact with the lateral border of the tongue and 25 clinically healthy individuals with normal teeth in contact with the lateral border of the tongue as a control group. Brush samples of epithelial cells were collected, then spread over clean glass slide and fixed in 100% alcohol, stained with rapid PAP and analyzed under the light microscope. The exfoliated cells were examined to detect micronuclei. Results. Micronuclei frequency was found to be increased in the study group where sharp teeth were in contact with the lateral border of the tongue of healthy individuals without deleterious habits such as usage of tobacco in smoking/smokeless form, areca nut chewing or alcohol consumption. Conclusions. Cytogenetic analysis is a simple and scantly invasive technique allowing clinicians the early detection of DNA damage in patients with sharp teeth and subsequently preventing carcinogenesis by proper treatment and follow-up.

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“…Several micronuclei assay studies in the past have brought out the association of micronuclei in genotoxic group, thus supporting the use of micronuclei assay as a potent biomarker for assessing DNA damage. [6][7][8][9][10] Previous studies have stated the hypothesis that, CMI could act at least as a co-factor in oral carcinogenesis and it is underrated as a potential risk factor. Tongue is the most common site for Sustained traumatic ulcers (Decubitus ulcer) and Traumatic ulcerative granuloma (Eosinophilic ulcer of the tongue).…”

Section: Discussionmentioning

confidence: 99%

“…They can be detected in exfoliated cells through cytological assay which involves the microscopic analysis of epithelial smears to determine the prevalence of genetic damage, an indicator of structural or numerical chromosomal aberrations. [6][7][8][9][10] With this background, this study was aimed to bring out the association of genetic damage (MN) along the lateral border of tongue in clinically healthy individuals with CoC.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic damage in individuals exposed to Chronic Mechanical Irritation (CMI) in the lateral border of the tongue due to Cusp of Carabelli (CoC) –A Cytogenetic study

prabhunath

Renugadevi²,

Chinthu³

et al. 2020

IJCRD

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Background: Chronic inflammation is estimated to contribute approximately 25% of human cancers. Inflammation, induced by chronic mechanical irritation (CMI) along with carcinogens has a higher chance of leading to oral pre-cancer and oral cancer. Though many studies have been conducted to identify the genetic damage in oral cancer or dysplastic stages, there is no data to find the early detection of genetic damage in healthy individuals associated with CMI. Cusp of Carabelli (CoC) is a small additional cusp at the mesiopalatal line angle of maxillary first molars which can cause chronic irritation to the lateral border of the tongue. Objective:The study is aimed to analyze genetic damage (micronuclei and nuclear buds) in healthy individuals associated with CoC over the lateral border of the tongue causing CMI. Materials and methods:The study comprised of 75 clinically healthy individuals with unilateral/bilateral presence of CoC causing irritation over the lateral border of the tongue as study group. Brush samples of lesional epithelial cells were collected, then spread over clean glass slide and fixed in 100% alcohol, stained with rapid PAP and analyzed under the light microscope. The exfoliated cells were examined to detect the presence of micronuclei (MN). Results: Incidence of MN was found to be more in CoC associated lateral border of the tongue of healthy individuals without habits. Conclusions: Cytogenetic analysis is a simple and scantly invasive technique allowing clinicians in early detection of DNA damage in patients with CoC and preventing the carcinogenesis by proper treatment and follow-up. .

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Micronuclei frequency as an early diagnostic tool for detection of Oral Cancer: A comparative study

Cited by 3 publications

References 0 publications

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Cytogenetic analysis of genetic damage in young individuals exposed to Chronic Mechanical Irritation in the lateral border of the tongue due to sharp teeth – A novel study.

Analysis of genetic damage in individuals exposed to Chronic Mechanical Irritation (CMI) in the lateral border of the tongue due to Cusp of Carabelli (CoC) –A Cytogenetic study

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