Micronuclei frequency in peripheral blood lymphocytes of thyroid cancer patients after radioiodine therapy and its relationship with metastasis

Cytotoxic and mutagenic effects of iodine-131 and radioprotection of acerola (Malpighia glabra L.) and beta-carotene in vitro

“…It is possible that an induction of these cellular defense mechanisms may have altered cell division; however, the nuclear division indexes (Table 1) Our data suggest that high concentrations of [ 131 I] (10 μCi) are mutagenic toward R. norvegicus HTC cultured, which agrees with previously published data from Watanabe et al (2004) and Joseph et al (2009); these studies also used the MN test to assess DNA damage and showed that there was significant DNA damage in human peripheral lymphocytes at the doses 100,000 and 10,000 to 850,000 μCi [ 131 I], respectively. These data indicate that higher doses cause greater damage to cells and have a dose-dependent effect, as indicated by Gutiérrez et al (1997).…”

“…Ionizing radiation also acts directly on nuclear DNA and may result in single-or double-strand breaks (Calegaro, 2007). Previous studies assessed the impact of various doses of radioiodine in several systems and indicated that isotope exposure increases the frequency of chromosomal aberrations (Puerto et al, 2000;da Silva et al, 2008;Düsman et al, 2011) and micronuclei (MN) (Watanabe et al, 2004;Joseph et al, 2009).…”

Correlation between Micronuclei Frequency in Peripheral Blood Lymphocytes and Retention of 131-I in Thyroid Cancer Patients

“…Large variations in the total radioactivity measured within the two groups of our patients (treated with 3.7 and 5.5 GBq, respectively) 72 h after the application of 131-I and considerable overlap between the values measured in patients who were treated with the two different doses of 131-I could explain the lack of influence of dose on MN frequency. The dose-independent increases of MN frequency in PBLs of DTC patients treated with 131-I were reported by Gutierrez et al (1999), Monsieurs et al (2000), and Joseph et al (2009), while Ballardin et al (2002) showed a dose-dependent effect of 131-I on MN frequency. Previous studies show an increased DNA damage in the PBLs of DTC patients treated with 131-I (Livingston et al 1993;Watanabe et al 1998;Ballardin et al 2002;Hooman et al 2008).…”

“…The published results showed discrepancies in the relationship between the administered 131-I activity and MN frequency. Several studies showed a dose-independent increase in MN frequency in patients with DTC treated with 131-I (Gutierrez et al 1999;Monsieurs et al 2000;Joseph et al 2009), while Ballardin et al (2002) showed a dose-dependent effect of 131-I on MN frequency. Because some of the applied iodine specifically bind and are retained in the residual thyroid tissue, while some iodine persist in other parts of the body for days after the therapy, giving very heterogeneous radiation exposure in patients treated with the same dose of 131-I (Hänscheid et al 2006(Hänscheid et al , 2009, the aim of our study was to examine whether the intensity of DNA damage measured by the cytokinesis-block micronucleus assay depends on the amount of 131-I that persists in the selected regions of interest (thyroid and abdominal region) as well as in the whole-body of DTC patients after therapy with two fixed activities (3.7 or 5.5 GBq) of 131-I.…”

Mutagenicity of diagnostic and therapeutical doses of radiopharmaceutical iodine-131 in Wistar rats

“…Radiation is known to affect cells directly or indirectly, interacting with genetic material and causing chromosomal aberrations or cell death (Joseph et al 2009). Ionizing radiation, through interaction with the environment, causes the formation of oxidizing free radicals by radiolysis of water, which react with intracellular macromolecules and alter cellular metabolism.…”